Abstract
The filamentous fungus Aspergillus niger produces a number of extracellular enzymes as well as primary and secondary metabolites, which are of considerable industrial interest. To facilitate molecular-genetic studies on the regulation of gene expression and protein secretion in this organism, the availability of an efficient gene-transfer system is of paramount importance. In this paper we describe the development of two such systems. The first one is a homologous transformation system, with a vector containing the A. niger pyrG gene as a selectable marker. In the second system, a vector containing the hygromycin B resistance gene as a dominant selectable marker is used for transformation. This system is also applicable to A. nidulans.
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© 1987 Plenum Press, New York
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Mattern, I. et al. (1987). New Gene-Transfer Systems for Aspergillus niger . In: Chaloupka, J., Krumphanzl, V. (eds) Extracellular Enzymes of Microorganisms. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1274-1_7
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DOI: https://doi.org/10.1007/978-1-4684-1274-1_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1276-5
Online ISBN: 978-1-4684-1274-1
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