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Clinical Studies with Recombinant-DNA-Derived Methionyl-Human Growth Hormone in Growth Hormone-Deficient Children

  • Chapter
Human Growth Hormone

Abstract

The advent of recombinant-DNA technology provides the prospect of synthesis of human growth hormone (hGH) in unlimited quantitites. Since 1% of protein synthesized by Escherichia coli by this procedure is hGH, the estimated yields are predicted to be substantial. The details of isolation of the mRNA and transcription of the DNA for hGH have been described earlier.(1–3) hGH has been cloned as a methionyl analogue.

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References

  1. D. V. Goeddel, H. L. Hyeneker, T. Hozum, R. Arentzen, K. Itakura, D. G. Yansura, M. J. Ross, G. Miozzari, R. Crea, and P. H. Seeburg, Direct expression in Eschericia coli of a DNA sequence coding for human growth hormone, Nature 281:544–548, 1979.

    Article  PubMed  CAS  Google Scholar 

  2. J. H. Martial, R. A. Hallewell, J. D. Baxter, and H. M. Goodman, Human growth hormone: Complementary DNA cloning and expression in bacteria, Science 205:602–607, 1979.

    Article  PubMed  CAS  Google Scholar 

  3. K. C. Olson, J. Fenno, N. Lin, R. N. Harkins, C. Snider, W. H. Kohr, M.J. Ross, D. Fodge, G. Prender, and N. Stebbing, Purified growth hormone from E. coli is biologically active, Nature 293:408–411, 1981.

    Article  PubMed  CAS  Google Scholar 

  4. R. G. Rosenfeld, B. B. Aggarwal, R. L. Hintz, and L. A. Dollar, Recombinant DNA-derived methionyl human growth hormone is similar in membrane binding properties to human pituitary growth hormone, Biochem. Biophys. Res. Commun. 106:202–209, 1982.

    Article  PubMed  CAS  Google Scholar 

  5. L. G. Frigeri, G. Robel, and N. Stebbing, Bacteria-derived human growth hormone lacks lipolytic activity in rat adipose tissue, Biochem. Biophys. Res. Commun. 104:1041–1046, 1982.

    Article  PubMed  CAS  Google Scholar 

  6. K. Takano, N. Hizuka, K. Shizume, K. Asakawa, and M. Kogawa, Short-term study of biosynthesized hGH in man, Endocrinol. Japan 30:(l):79–84, 1983.

    Article  CAS  Google Scholar 

  7. R. L. Hintz, R. G. Rosenfeld, D. M. Wilson, A. Bennett, J. Finno, B. McClellan, and R. Swift, Biosynthetic methionyl human growth hormone is biologically active in adult man, Lancet 1:1276–1279, 1982.

    Article  PubMed  CAS  Google Scholar 

  8. R. G. Rosenfeld, D. M. Wilson, L. A. Dollar, A. Bennett, and R. Hintz, Both human pituitary growth hormone and recombinant DNA-derived human growth hormone cause insulin resistance at a postreceptor site, J. Clin. Endocrinol. Metab. 54:1033–1038, 1982.

    Article  PubMed  CAS  Google Scholar 

  9. L. E. Underwood, S. J. Voina, and J. J. Van Wyk, Restoration of growth by human growth hormone (Roos) in hypopituitary dwarfs immunized by other human growth hormone preparations: Clinical and ummunological studies, Clin. Endocrinol. Metab. 38:288–297, 1974.

    Article  CAS  Google Scholar 

  10. H. G. Goodman, M. M. Grumbach, and S. L. Kaplan, Growth and growth hormone. II. A comparison of isolated growth-hormone deficiency and multiple pituitary-hormone deficiencies in 35 patients with idiopathic hypopituitary dwarfism, N. Engl. J. Med. 278:57–68, 1968.

    Article  PubMed  CAS  Google Scholar 

  11. M. A. Preece, J. M. Tanner, R. H. Whitehouse, and N. Cameron, Dose dependence of growth response to human growth hormone in growth hormone deficiency, J. Clin. Endocrinol. Metab. 42:477–483, 1976.

    Article  PubMed  CAS  Google Scholar 

  12. S. D. Frasier, Human pituitary growth hormone (HGH) therapy in growth hormone deficiency, Endocrine Rev. 4:155–170, 1983.

    Article  CAS  Google Scholar 

  13. T. Aceto, Jr., S. D. Frasier, A. B. Hayles, H. I. L. Meyer-Bahlburg, M. L. Parker, R. Munschauer, and G. Di Chiro, Collaborative study of the effects of human growth hormone in growth hormone deficiency. I. First year of therapy, J. Clin. Endocrinol. Metab. 35:483–496, 1972.

    Article  PubMed  Google Scholar 

  14. D. M. Bergenstal, and M. B. Lipsett, Metabolic effects of human growth hormone and growth hormone of other species in man, J. Clin. Endocrinol. Metab. 20:1427–1436, 1960.

    Article  CAS  Google Scholar 

  15. A.J. D’Ercole, M. D. Underwood, and J. J. Van Wyk, Serum somatomedin-C in hypopituitarism and in other disorders of growth, J. Pediatr. 90:375–381, 1977.

    Article  PubMed  Google Scholar 

  16. K. C. Copeland, L. E. Underwood, and J. J. Van Wyk, Induction of immunoreactive somatomedin C in human serum by growth hormone: dose-response relationships and effect of chromatographic profiles, J. Clin. Endocrinol. Metab. 50:690–697, 1980.

    Article  PubMed  CAS  Google Scholar 

  17. R. G. Rosenfeld, S. F. Kemp, and R. L. Hintz, Constancy of somatomedin response to growth hormone treatment of hypopituitary dwarfism, and lack of correlation with growth rate, J. Clin. Endocrinol. Metab. 53:611–617, 1981.

    Article  PubMed  CAS  Google Scholar 

  18. S. L. Blethen, W. H. Daughaday, and V. V. Weldon, Kinetics of the somatomedin C/insulin-like growth factor I: Response to exogenous growth hormone (GH) in GH deficient children, J. Clin. Endocrinol. Metab. 54:986–990, 1982.

    Article  PubMed  CAS  Google Scholar 

  19. L. A. Frohman, M. H. MacGillivray, and T. Aceto, Jr., Acute effects of human growth hormone on insulin secretion and glucose utilization in normal and GH deficient states, J. Clin. Endocrinol. Metab. 27:561–567, 1967.

    Article  PubMed  CAS  Google Scholar 

  20. H. Gold, S. Spector, N. A. Samaan, and O. Pearson, Effect of growth hormone on carbohydrate metabolism in hypopituitary dwarfs, Metabolism 17:74–83, 1983.

    Article  Google Scholar 

  21. G. Costin, M. D. Kogut, and S. D. Frasier, Effect of low dose human growth hormone on carbohydrate metabolism in children with hypopituitarism, J. Pediatr. 80:796–803, 1972.

    Article  PubMed  CAS  Google Scholar 

  22. L. E. Underwood, J. L. Van der Brande, G. J. Anthony, S. J. Voina, and J. J. Van Wyk, Islet cell function and glucose homeostasis in hypopituitary dwarfism: Synergism between growth hormone and cortisone, J. Pediatr. 82:28–37, 1973.

    Article  PubMed  CAS  Google Scholar 

  23. B. M. Lippe, S. A. Kaplan, M. P. Golden, S. A. Hendricks, and M. L. Scott, Carbohydrate tolerance and insulin binding in children with hypopituitarism: Response after acute and chronic human growth hormone administration, J. Clin. Endocrinol. Metab. 53:507–513, 1981.

    Article  PubMed  CAS  Google Scholar 

  24. A. W. Root, A. M. Bongiovanni, and W. R. Eberlein, Inhibition of thyroidal radioiodine uptake by human growth hormone, J. Pediatr. 76:422–429, 1970.

    Article  PubMed  CAS  Google Scholar 

  25. B. A. Porter, S. Refetoff, R. L. Rosenfeld, L. J. De Groot, V. S. Fang, and V. Stark, Abnormal thyroxine metabolism in hyposomatotrophic dwarfism and inhibition of responsiveness to TRH during GH therapy, Pediatrics 51:668–674.

    Google Scholar 

  26. B. M. Lippe, A. J. Van Herle, S. H. La Franchi, R. P. Uller, N. Lavin, and S. A. Kaplan, Reversible hypothyroidism in growth hormone deficient children treated with human growth hormone, J. Clin. Endocrinol. Metab. 40:612–618, 1975.

    Article  PubMed  CAS  Google Scholar 

  27. A. Prader, J. Szeky, H. Wagner, R. Illig, J. L. Touber, and D. Maingoy, Acquired resistance to human growth hormone caused by specific antibodies, Lancet 2:378–382, 1964.

    Article  PubMed  CAS  Google Scholar 

  28. R. Illig, Growth hormone antibodies in patients treated with different preparations of human growth hormone (HGH), J. Clin. Endocrinol. 31:679–688, 1970.

    Article  CAS  Google Scholar 

  29. S. L. Kaplan, D. C. L. Savage, S. Suter, R. Wolter, and M. M. Grumbach, Antibodies to human growth hormone arising in patients treated with human growth hormone: incidence, characteristics, and effects on growth, in: Advances in Human Growth Hormone Research; A Symposium, U. S. Department of Health, Education and Welfare, Publication No. NIH 74–612, 725747, 1974.

    Google Scholar 

  30. S. D. Frasier, T. Aceto Jr., A. B. Hayles, M. L. Parker, and H. F. L. Meyer-Bahlburg, Collaborative study of the effects of human growth hormone in growth hormone deficiency. II. Development and significance of antibodies to human growth hormone during the first year of therapy, J. Clin. Endocrinol. Metab. 38:14–18, 1974.

    Article  PubMed  CAS  Google Scholar 

  31. W. V. Moore and P. Leppert, Role of aggregated human growth hormone (hGH) in development of antibodies to hGH, J. Clin. Endocrinol. Metab. 51:691–697, 1980.

    Article  PubMed  CAS  Google Scholar 

  32. O. Westphal and P. Roos, Treatment of pituitary dwarfism with human growth hormone, Acta. Paediatr. Scand 182(Suppl):52–81, 1968.

    Google Scholar 

  33. G. Van Vliet, D. M. Styne, S. L. Kaplan, and M. M. Grumbach, Growth hormone treatment for short stature, New Engl. J. Med. 309:1016–1022, 1983.

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Pediatrics, University of California-San Francisco, San Francisco, California, 94143, USA

    S. L. Kaplan

  2. Department of Pediatrics, University of North Carolina School of Medicine, Chapel Hill, North Carolina, 27514, USA

    L. E. Underwood

  3. Department of Pediatrics, National Medical Center, Children’s Hospital, Washington, D.C., 20010, USA

    G. P. August

  4. Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York, 10032, USA

    J. J. Bell

  5. Department of Pediatrics, Washington University School of Medicine, Saint Louis, Missouri, 63110, USA

    S. L. Blethen

  6. Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, 55455, USA

    D. R. Brown

  7. Department of Pediatrics, University of Pittsburgh, Pittsburgh, Pennsylvania, 15213, USA

    T. P. Foley

  8. Department of Pediatrics, Stanford University, Stanford, California, 94305, USA

    R. L. Hintz

  9. Department of Pediatrics, University of Michigan, Ann Arbor, Michigan, 48109, USA

    N. J. Hopwood

  10. Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, Virginia, 22901, USA

    A. J. Johanson

  11. Department of Pediatrics, University of Texas, Houston, Texas, 77030, USA

    R. T. Kirkland

  12. Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205, USA

    L. P. Plotnick

Authors
  1. S. L. Kaplan
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  2. L. E. Underwood
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  4. J. J. Bell
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  5. S. L. Blethen
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  8. R. L. Hintz
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Editor information

Editors and Affiliations

  1. National Hormone and Pituitary Program, Baltimore, Maryland, USA

    Salvatore Raiti

  2. National Institute of Arthritis, Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland, USA

    Robert A. Tolman

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© 1986 Plenum Publishing Corporation

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Kaplan, S.L. et al. (1986). Clinical Studies with Recombinant-DNA-Derived Methionyl-Human Growth Hormone in Growth Hormone-Deficient Children. In: Raiti, S., Tolman, R.A. (eds) Human Growth Hormone. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7201-5_22

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  • DOI: https://doi.org/10.1007/978-1-4615-7201-5_22

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-7203-9

  • Online ISBN: 978-1-4615-7201-5

  • eBook Packages: Springer Book Archive

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