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Mansonones, Elicitors and Virulence

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Dutch Elm Disease Research

Abstract

Mansonones may constitute an effective systemic defense system of elms against Ophiostoma ulmi, the causal agent of Dutch elm disease. They are produced in response to fungal invasion, cause at 0.1 mM cellular ion leakage and reduce the rate of respiration of the pathogen. Changes in the ultrastructure of the fungus by these compounds include membrane disruption, ribosomal aggregation and aggregation of electron dense material in mitochondria. There seems to exist a correlation between pathogen virulence and mansonone production. The more virulent the strains are, the less mansonone they induce in elm seedlings. Fungal culture filtrates, cytoplasm and cell walls of O. ulmi contain molecules that strongly stimulate elm callus cells to produce mansonones. The production of mansonones is influenced by elicitor concentration, the callus culture conditions and callus subculturing frequency. A 21,000 dalton glycoprotein, identified as mansonone-inducing elicitor in elm callus, was purified from culture filtrate using both isoelectrofocusing and HPLC ion exchange chromatography. Western blotting with the polyclonal antiserum against the elicitor reacted only with the glycoprotein. Work on the sequence of the elicitor and its isolation from the genomic library of the fungus is in progress. Experiments are also carried out to determine whether cerato-ulmin, a toxin produced by O. ulmi plays a key role in pathogen virulence.

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Authors
  1. Martin Hubbes
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Editor information

Editors and Affiliations

  1. Pesticide Research Center, Michigan State University, East Lansing, MI, 48824, USA

    Mariam B. Sticklen

  2. National Park Service, Washington, DC, 20242, USA

    James L. Sherald

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© 1993 Springer-Verlag New York, Inc.

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Hubbes, M. (1993). Mansonones, Elicitors and Virulence. In: Sticklen, M.B., Sherald, J.L. (eds) Dutch Elm Disease Research. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-6872-8_17

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  • DOI: https://doi.org/10.1007/978-1-4615-6872-8_17

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-6874-2

  • Online ISBN: 978-1-4615-6872-8

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