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The dynamic regulation of myocardial oxidative phosphorylation: Analysis of the response time of oxygen consumption

  • Chapter
Bioenergetics of the Cell: Quantitative Aspects

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 25))

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Abstract

Although usually steady-state fluxes and metabolite levels are assessed for the study of metabolic regulation, much can be learned from studying the transient response during quick changes of an input to the system. To this end we study the transient response of O2 consumption in the heart during steps in heart rate. The time course is characterized by the mean response time of O2 consumption which is the first statistical moment of the impulse response function of the system (for mono-exponential responses equal to the time constant). The time course of O2 uptake during quick changes is measured with O2 electrodes in the arterial perfusate and venous effluent of the heart, but the venous signal is delayed with respect to O2 consumption in the mitochondria due to O2 diffusion and vascular transport. We correct for this transport delay by using the mass balance of O2, with all terms (e.g. O2 consumption and vascular O2 transport) taken as function of time. Integration of this mass balance over the duration of the response yields a relation between the mean transit time for O2 and changes in cardiac O2 content. Experimental data on the response times of venous [O2] during step changes in arterial [O2] or in perfusion flow are used to calculate the transport time between mitochondria and the venous O2 electrode. By subtracting the transport time from the response time measured in the venous outflow the mean response time of mitochondrial O2 consumption (tmito) to the step in heart rate is obtained.

In isolated rabbit heart we found that tmito to heart rate steps is 4–12 s at 37°C. This means that oxidative phosphorylation responds to changing ATP hydrolysis with some delay, so that the phosphocreatine levels in the heart must be decreased, at least in the early stages after an increase in cardiac ATP hydrolysis. Changes in ADP and inorganic phosphate (Pi) thus play a role in regulating the dynamic adaptation of oxidative phosphorylation, although most steady state NMR measurements in the heart had suggested that ADP and Pi do not change. Indeed, we found with 31P-NMR spectroscopy that phosphocreatine (PCr) and Pi. change in the first seconds after a quick change in ATP hydrolysis, but remarkably they do this significantly faster (time constant ∼2.5 s) than mitochondrial O2 consumption (time constant 12 s). Although it is quite likely that other factors besides ADP and Pi. regulate cardiac oxidative phosphorylation, a fascinating alternative explanation is that the first changes in PCr measured with NMR spectroscopy took exclusively place in or near the myofibrils, and that a metabolic wave must then travel with some delay to the mitochondria to stimulate oxidative phosphorylation. The tmito slows with falling temperature, intracellular acidosis, and sometimes also during reperfusion following ischemia and with decreased mitochondrial aerobic capacity. In conclusion, the study of the dynamic adaptation of cardiac oxidative phosphorylation to demand using the mean response time of cardiac mitochondrial O2 consumption is a very valuable tool to investigate the regulation of cardiac mitochondrial energy metabolism in health and disease.(Mol Cell Biochem 184: 321–344, 1998)

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References

  1. Van Beek JHGM, Elzinga G: Response time of mitochondrial oxygen consumption to heart rate changes in isolated rabbit heart. Proc. Int. Union Physiol Sci 16:485, 1986

    Google Scholar 

  2. Van Beek JHGM, Westerhof N: Response time of mitochondrial oxygen consumption following stepwise changes in cardiac energy demand. In: J Piiper, TK Goldstick, M Meyer (eds). Oxygen Transport to Tissue XII. Plenum Press, New York, 1990, pp 415–423

    Chapter  Google Scholar 

  3. Van Beek JHGM, Westerhof N: Response time of cardiac mitochondrial oxygen consumption to heart rate steps. Am J Physiol 260:H613–H625, 1991

    PubMed  Google Scholar 

  4. Groot B de, Zuurbier CJ, Beek JHGM van: Response times of mitochondrial oxygen consumption, tissue oxygenation and venous oxygen concentration during steps in heart rate in isolated rabbit heart. Pflügers Arch. 430(S4): R93, 1995

    Google Scholar 

  5. Beek JHGM van, Osbakken MD, Chance B: Measurement of the oxygenation status of the isolated perfused rat heart using near infrared detection. Adv Exp Med Biol 388:147–154, 1996

    Article  PubMed  Google Scholar 

  6. Mast F, Elzinga G: Recovery heat production of isolated rabbit papillary muscle at 20°C. Pflügers Arch 411:600–605, 1988

    Article  PubMed  CAS  Google Scholar 

  7. Zuurbier CJ, Mast F, Elzinga G, Beek JHGM van: Mitochondrial function is not decreased in stunned papillary muscle at 20°C. J Moll Cell Cardiol 29:347–355, 1997

    Article  CAS  Google Scholar 

  8. Kushmerick MJ, Meyer RA: Chemical changes in rat leg muscle by phosphorus nuclear magnetic resonance. Am J Physiol 248:C542–549, 1985

    PubMed  CAS  Google Scholar 

  9. Meyer RA: A linear model of muscle respiration explains mono-exponential phosphocreatine changes. Am J Physiol 254:C548–C553, 1988

    PubMed  CAS  Google Scholar 

  10. Massie B, Schwartz GG, Garcia J, Wisneski JA, Weiner MW, Owens T: Myocardial metabolism during increased work states in the porcine left ventricle in vivo. Circ Res 74:64–73, 1994

    Article  PubMed  CAS  Google Scholar 

  11. Balaban RS, Kantor HL, Katz LA, Briggs RW: Relation between work and phosphate metabolites in the in vivo paced mammalian heart. Science 232:1121–1123, 1986

    Article  PubMed  CAS  Google Scholar 

  12. Balaban RS: Regulation of mitochondrial oxidative phosphorylation in mammalian cells. Am J Physiol 258:C377–389, 1990

    PubMed  CAS  Google Scholar 

  13. Chance B: Reaction of oxygen with the respiratory chain in cells and tissues. J Gen Physiol 49:163–188, 1966

    Article  Google Scholar 

  14. Saks VA, Khuchua ZA, Vasilyeva EV, Belikova OY, Kuzuetsov AV: Metabolic compartmentation and substrate channelling in muscle cells. Role of coupled creatine kineses in in vivo regulation of cellular respiration—a synthesis. Moll Cell Biochem 133/134:155–192, 1994

    Article  Google Scholar 

  15. Beek, JHGM van: Dynamics of cardiac mitochondrial oxygen consumption deduced from whole organ oxygen uptake. In: JB Bassingthwaighte, J Linehan (eds). Whole OrganApproach to Cellular Metabolism: Capillary Permeation, Cellular Transport and Reaction Kinetics, Springer, New York, in press

    Google Scholar 

  16. Lassen NA, Henriksen O, Sejrsen P: Indicator methods for measurement of organ and tissue blood flow. In: JT Shepherd, FM Abboud (eds). Handbook of Physiology. Section 2: The Cardiovascular System. Vol. III. Peripheral Circulation and Organ Blood Flow. Part 1, 1983, pp 21–63

    Google Scholar 

  17. Meier P, Zierler KL: On the theory of the indicator-dilution method for measurement of blood flow and volume. J Appl Physiol 6: 731–744,;1954

    PubMed  CAS  Google Scholar 

  18. Roberts GW, Larson KB, Spaeth EE: The interpretation of mean transit time measurements for multiphase tissue systems. J Theor Biol 39:447–475, 1973

    Article  PubMed  CAS  Google Scholar 

  19. Loiselle DS, Van Beek JHGM, Mawson DA, Hunter PJ: The surface of the heart leaks oxygen. News Physiol Sciences 10:129–133, 1995

    Google Scholar 

  20. Van Beek JHGM, Loiselle DS, Westerhof N: Calculation of oxygen diffusion across the surface of isolated perfused hearts. Am J Physiol 263: H1003–1010, 1992

    PubMed  Google Scholar 

  21. Beauvoit B, Chance B: Time-resolved spectroscopy of mitochondria, cells and tissues under normal and pathological conditions. This volume.

    Google Scholar 

  22. Heinrich R, Rapoport TA: Mathematical analysis of multienzyme systems: II. Steady state and transient control. Biosystems 7:130–136, 1975

    Article  PubMed  CAS  Google Scholar 

  23. King RB, Bassingthwaighte JB, Hales JRS, Rowell LB: Stability of heterogeneity of myocardial blood flow in normal awake baboons. Circ Res 57:285–295, 1985

    Article  PubMed  CAS  Google Scholar 

  24. Gorman MW, Wangler RD, Sparks HV: Distribution of perfusate flow during vasodilation in isolated guinea pig heart. Am J Physiol 256:H297–301, 1989

    PubMed  CAS  Google Scholar 

  25. Beek JHGM van, Bussemaker J, Barends JPF, Westerhof N: A 13C-NMR technique to determine absolute oxygen consumption in quickly frozen small myocardial samples. FASEB J 10: A325, 1996

    Google Scholar 

  26. Van Beek JHGM: Fractal models of heterogeneity in organ blood flow. In: S Egginton, H Ross (eds). Oxygen Transport in Biological Systems: modelling of pathways from environment to cell. Cambridge Uni versity Press, Cambridge, 1992, pp 135–163

    Google Scholar 

  27. Van Beek JHGM, Roger SA, Bassingthwaighte JB: Regional myo cardial flow heterogeneity explained with fractal networks. Am J Physiol 257:H1670–1680, 1989

    PubMed  Google Scholar 

  28. Van Beek JHGM, Barends JPF, and Westerhof N: The microvascular unit size for fractal flow heterogeneity relevant for oxygen transport. In: P Vaupel, R Zander, DF Bruley (eds). Oxygen Transport to Tissue XV, 1994, pp 901–908

    Google Scholar 

  29. Hak JB, Biessels PTM, Beek JHGM van, Bakker JC, and Westerhof N: Function of isolated rabbit hearts perfused with erythrocyte suspension is not stable but improvement may be feasible with hemoglobin solution. Adv Exp Med Biol 345:291–297, 1994

    Article  PubMed  CAS  Google Scholar 

  30. Hak JB, Van Beek JHGM, Wijhe MH van, Westerhof N: Influence of temperature on the response time of mitochondrial oxygen con sumption in isolated rabbit heart. J Physiol (Lond) 447:17–31, 1992

    CAS  Google Scholar 

  31. Wittenberg JB: Myoglobin-facilitated oxygen diffusion: role of myoglobin in oxygen entry into muscle. Physiol Rev 50:559–636, 1970

    PubMed  CAS  Google Scholar 

  32. Zuurbier CJ, Beek JHGM van: Brief ischemia results in decreased mitochondrial function and contractility in isolated rabbit heart at 37°C. FASEB J 9, 1995

    Google Scholar 

  33. Eijgelshoven MHJ, Van Beek JHGM, Mottet I, Nederhoff MGJ, Van Echteld CJA, Westerhof N: Cardiac high-energy phosphates adapt faster than oxygen consumption to changes in heart rate. Circ Res 75:751–759, 1994

    Article  PubMed  CAS  Google Scholar 

  34. Chance B: The energy-linked reaction of calcium with mitochondria. J Biol Chem 240:2729–2748, 1965

    PubMed  CAS  Google Scholar 

  35. Lemasters JJ, Hackenbrock CR: Continuous measurement and rapid kinetics of ATP synthesis in rat liver mitochondria, mitoplasts and inner membrane vesicles determined by firefly-luciferase luminescence. Eur J Biochem 67:1–10, 1976

    Article  PubMed  CAS  Google Scholar 

  36. Eijgelshoven MHJ, Lekkerkerk C, Van Beek JHGM, Van Echteld CJA: Oxygen measurement inside an NMR magnet with a catheter electrode. Magn Res Med 29:559–562, 1993

    Article  CAS  Google Scholar 

  37. Kingsley-Hickman PB, Sako EY, Mohanakrishnan P, Robitaille PML, From AHL, Foker JE, Ugurbil K: 31P NMR studies of ATP synthesis and hydrolysis kinetics in the intact myocardium. Biochem 26:7501–7510, 1987

    Article  CAS  Google Scholar 

  38. Connett RJ, Gayeski TEJ, Honig CR: Energy sources in fully aerobic rest-work transitions: a new role for glycolysis. Am J Physiol 248:H922–H929, 1985

    PubMed  CAS  Google Scholar 

  39. Hak JB, Van Beek JHGM, van Wijhe MH, Westerhof N: Dynamics of myocardial lactate effflux after a step in heart rate in isolated rabbit heart. Am J Physiol 265:H2081–2085, 1993

    PubMed  CAS  Google Scholar 

  40. Eijgelshoven MHJ: Dynamics of cardiac energy metabolism. (thesis), Free University, Amsterdam, 1995, ISBN 90-9007857-6

    Google Scholar 

  41. Katz LA, Koretsky AP, Balaban RS: Activation of dehydrogenase activity and cardiac respiration: a 31P-NMR study. Am J Physiol 255:H185–188, 1988

    PubMed  CAS  Google Scholar 

  42. Heineman FW, Balaban RS: Phosphorus-31 nuclear magnetic resonance analysis of transient changes of canine myocardial metabolism in vivo. J Clinic Invest 85:843–852, 1990

    Article  CAS  Google Scholar 

  43. McCormack JG, Denton RM: Ca2+ as a second messenger within mitochondria. Trends Biochem Sci 11:258–262, 1986

    Article  CAS  Google Scholar 

  44. Hansford RG: Relation between mitochondrial calcium transport and control of energy metabolism. Rev Physiol Biochem Pharmacol 102:1–72, 1985

    Article  PubMed  CAS  Google Scholar 

  45. Eng J, Lynch RM, Balaban RS: Nicotinamide adenine dinucleotide fluorescence spectroscopy and imaging of isolated cardiac myocytes. Biophys J 55:621–630, 1989

    Article  PubMed  CAS  Google Scholar 

  46. Katz LA, Koretsky AP, Balaban RS: Respiratory control in the glucose perfused heart: a 31P-NMR and NADH fluorescence study. FEB S Lett 221:270–276, 1987

    Article  CAS  Google Scholar 

  47. Heineman FW, Kupriyanov W, Marshall R, Fralix TA, Balaban RS: Myocardial oxygenation in the isolated working rabbit heart as a function of work. Am J Physiol 262:H255–267, 1992

    PubMed  CAS  Google Scholar 

  48. Heineman FW, Balaban RS: Effects of afterload and heart rate on NAD(P)H redox state in the isolated rabbit heart. Am J Physiol 264:H433–440, 1993

    PubMed  CAS  Google Scholar 

  49. Chapman JB: Fluorometric studies of oxidative metabolism in isolated papillary muscle of the rabbit. J Gen Physiol 59:135–154, 1972

    Article  PubMed  CAS  Google Scholar 

  50. White RL, Wittenberg BA: NADH fluorescence of isolated ventricular myocytes: effects of pacing, myoglobin and oxygen supply. Biophys J 65:196–204, 1993

    Article  PubMed  CAS  Google Scholar 

  51. Ashruf JF, Coremans JMCC, Bruining HA, Ince C: Increase of cardiac work is associated with decrease of mitochondrial NADH in the Langendorff rat heart. Am J Physiol 269:H856–862, 1995

    PubMed  CAS  Google Scholar 

  52. Miyata H, Silverman HS, Sollott SJ, Lakatta EG, Stern MD, Hansford RG: Measurement of mitochondrial free Ca2+ concentration in living single rat cardiac myocytes. Am J Physiol 261:H1123–H1134, 1991

    PubMed  CAS  Google Scholar 

  53. Brandes R, Bers D: Intracellular Ca2+ increases the mitochondrial NADH concentration during elevated work in intact cardiac muscle. Circ Res 80:82–87, 1997

    Article  PubMed  CAS  Google Scholar 

  54. Harris DA, Das AM: Control of mitochondrial ATP synthesis in the heart. Biochem J 280:561–573, 1991

    PubMed  CAS  Google Scholar 

  55. Hak JB, Van Beek JHGM, Eijgelshoven MHJ, Westerhof N: Mito chondrial dehydrogenase activity affects adaptation of cardiac oxygen consumption to demand. Am J Physiol 264:H448–453, 1993

    PubMed  CAS  Google Scholar 

  56. Elliott AC, Smith GL, Allen DG: The metabolic consequences of an increase in the frequency of stimulation in isolated ferret hearts. J Physiol (Lond) 474:147–159, 1994

    CAS  Google Scholar 

  57. Wan B, Doumen C, Duszynski, Salama G, Vary TC, LaNoue KF: Effects of cardiac work on electrical potential gradient across mitochondrial membrane in perfused rat hearts. Am J Physiol 265:H453–460, 1993

    PubMed  CAS  Google Scholar 

  58. From AHL, Zimmer SD, Michurski SP, Mohanokrishnan P, Ulstadt VK, Thoma WJ, Ugurbil K: Regulation of the oxidative phosphory-lation in the intact cell. Biochem 29:3731–3743, 1990

    Article  CAS  Google Scholar 

  59. Kim DL, Heineman FW, Balaban RS: Effects of-hydroxybutyrate on oxidative metabolism and phosphorylation potential in canine heart in vivo. Am J Physiol 260:H1767–1773, 1991

    PubMed  CAS  Google Scholar 

  60. Eijgelshoven MHJ, Hak JB, Van Beek JHGM, Westerhof N: Adaptation speed of cardiac mitochondrial oxygen consumption to demand slows with higher hears rate. Am J Physiol 265: H1893–1898, 1993.

    PubMed  CAS  Google Scholar 

  61. Eijgelshoven MHJ, Tian X, Van Beek JHGM: Exogenous carbon substrate supply affects the time course of adaptation of cardiac mitochondrial oxygen consumption to demand in a temperature dependent fashion. In: MHJ Eijgelshoven. Dynamics of cardiac energy metabolism, (thesis), Free University, Amsterdam, 1995, ISBN 90-9007857-6, pp 73–98

    Google Scholar 

  62. Eijgelshoven MHJ, Tian X, Westerhof N, van Beek JHGM: Is the adaptation speed of myocardial oxidative phosphorylation to heart rate steps affected by the type of exogenous substrate? J. Physiol. 479P: 72–73P, 1994

    Google Scholar 

  63. Isaacs GH, Sacktor B, Murphy TA: The role of the a-glycerophosphate cycle in the control of carbohydrate oxidation in heart and in the mechanism of action of thyroid hormone. Biochim BiophysActa 177:196–203, 1969

    Article  CAS  Google Scholar 

  64. Zizi M, Forte M, Blachly-Dyson E, Colombini M: NADH regulates the gating of VDAC, the mitochondrial outer membrane channel. J Biol Chem 269:1614–1616, 1994.

    PubMed  CAS  Google Scholar 

  65. Tian X, van Beek JHGM, Eijgelshoven MHJ: Effects of inotropic stimulation and adenosine on the response time of cardiac mito chondrial oxygen consumption to heart rate steps in the isolated rabbit heart. J Physiol (Lond) 479: P 132, 1994

    Google Scholar 

  66. Bessman SP, Geiger PJ: Transport of energy in muscle: the phos-phorylcreatine shuttle. Science 211:448–452, 1981

    Article  PubMed  CAS  Google Scholar 

  67. Meyer RA, Sweeney HL, Kuskmerick MJ: A simple analysis of the ‘phosphocreatine shuttle’. Am J Physiol 246:C365–377, 1984

    PubMed  CAS  Google Scholar 

  68. Mahler M: First order kinetics of muscle oxygen consumption and an equivalent proportionality between QO2 and phosphorylcreatine level. Implications for the control of respiration. J Gen Physiol 86:135–165, 1985

    Article  PubMed  CAS  Google Scholar 

  69. van Beek JHGM, Eijgelshoven MHJ, Hak JB, Westerhof N: Control loop gain of cardiac oxidative phosphorylation via phosphate metabolites is high and partially explains the dynamic adaptation of oxygen consumption to demand. FASEB J 7: A313, 1993.

    Google Scholar 

  70. Gyulay L, Roth Z, Leigh JS Jr., Chance B: Bioenergetic studies of mitochondrial oxidative phosphorylation using 31phosphorus NMR. J Biol Chem 260:3947–3954, 1985

    Google Scholar 

  71. Holian A, Owen CS, Wilson DF: Control of respiration in isolated mitochondria: quantitative evaluation of the dependence of respiratory rates on [ATP], [ADP] and [Pi]. Arch Biochem Biophys 181:164–171, 1977

    Article  PubMed  CAS  Google Scholar 

  72. Ugurbil K, Petein M, Maidan R, Michurski S, FromAHL: Measurement of an individual rate constant in the presence of multiple exchanges: application to myocardial creatine kinase reaction. Biochem 25:100–107, 1986

    Article  CAS  Google Scholar 

  73. Bittl JA, Ingwall JS: Reaction rates of creatine kinase and ATP synthesis in the isolated rat heart. J Biol Chem 260:3512–3517, 1985

    PubMed  CAS  Google Scholar 

  74. Conley CK, Kushmerick MJ: Buffering work transitions in myo cardium: role of a poorly metabolized creatine analogue. Book of Abstracts. Society of Magnetic Resonance in Medicine. 9th Annual Meeting, p. 902, 1990

    Google Scholar 

  75. Unitt JF, Schrader J, Brunotte F, Radda GK, Seymour AML: Deter mination of free creatine and phosphocreatine in the isolated perfused rat heart by 1H-and 31P-NMR. Biochim Biophys Acta 1133:115–120, 1992

    Article  PubMed  CAS  Google Scholar 

  76. Gellerich FN, Khuchua ZA, Kuznetsov AV: Influence of the mitochond rial outer membrane and the binding of creatine kinase to the mitochondrial inner membrane on the compartmentation of adenine nucleotides in the intermembrane space of rat heart mitochondria. Biochim BiophysActa 1140:327–334, 1993

    Article  CAS  Google Scholar 

  77. Gellerich FN, Wagner M, Kapischke M, Brdiczka D: Effect of macromolecules onADP-channeling into mitochondria. In: JP Mazat. Proceedings of the 5th BTK-Workshop. Plenum, London, 1993

    Google Scholar 

  78. Saks VA, Vasileva E, Belikova YO, Kuznetsov AV, Lyapina S, Petrova L, Perov NA: Retarded diffusion of ADP in cardiomyocytes: possible role of mitochondrial outer membrane and creatine kinase in cellular regulation of oxidative phosphorylation. Biochim BiophysActa 1144:134–148, 1993

    Article  CAS  Google Scholar 

  79. Jeneson JAL, Wiseman RW, Westerhoff HV, Kushmerick MJ: Amplified ADP sensitivity of mitochondria. In: HV Westerhoff, JL Snoep, JE Wijker, FE Sluse, BN Kholodenko (eds). BioThermoKinetics of the Living Cell. BioThermoKinetics Press, Amsterdam, 1996, pp 94–95

    Google Scholar 

  80. O’Rourke B, Ramza BM, Marban E: Oscillations of membrane current and excitability driven by metabolic oscillations in heart cells. Science 265:962–966, 1994

    Article  CAS  Google Scholar 

  81. Cristea E: Effect of amytal, pyridine nucleotides, and oxaloacetate on the respiratory inhibition-activation cycles in mitochondria. Arch Biochem Biophys 113:273–280, 1966

    Article  PubMed  CAS  Google Scholar 

  82. Chance B, Williamson JR, Jamieson D, Schoener B: Properties and kinetics of reduced pyridine nucleotide fluorescence of the isolated and in vivo rat heart. Biochem Z 341:357–377, 1965

    Google Scholar 

  83. Van der Laarse WJ, Diegenbach PC, Elzinga G: Maximum rate of oxygen consumption and quantitative histochemistry of succinate dehydrogenase in single muscle fibres of Xenopus laevis. J Muscle Res Cell Motil 10:221–228, 1989

    Article  PubMed  Google Scholar 

  84. Gollnick PD, Bertocci LA, Kelso TB, Witt EH, Hodgson DR: The effect of high-intensity exercise on the respiratory capacity of skeletal muscle. Pflügers Arch 415:407–413, 1990

    Article  PubMed  CAS  Google Scholar 

  85. Hak JB, Van Beek JHGM, Westerhof N: Acidosis slows the response of oxidative phosphorylation to metabolic demand in isolated rabbit heart. Pflügers Arch. 423:324–329, 1993

    Article  PubMed  CAS  Google Scholar 

  86. Nakanishi T, Seguchi M, Tsuchiya T, Yasukuochi S, Takao A: Effect of acidosis on intracellular pH and calcium concentrations in the newborn and adult rabbit myocardium. Circ Res 67:111–123, 1990

    Article  PubMed  CAS  Google Scholar 

  87. van Wijhe MH, van Beek JHGM: Effects of respiratory and metabolic acidosis on oxidative phosphorylation in isolated rabbit heart mitochondria. Europ J Physiol 430: R174, 1995

    Google Scholar 

  88. Flameng W, Andres J, Ferdinande P, Mattheussen M, Van Belle H: Mitochondrial function in myocardial stunning. J Moll Cell Cardiol 23:1–11, 1991

    Article  CAS  Google Scholar 

  89. Mekhfi H, Veksler V, Mateo P, Maupoil V, Rochette L, Ventura-Clapier R: Creatine kinase is the main target of reactive oxygen species in cardiac myofibrils. Circ Res 78:1016–1027, 1996

    Article  PubMed  CAS  Google Scholar 

  90. Bittl JA, Weisfeldt ML, Jacobus WE: Creatine kinase of heart mitochondria. The progressive loss of enzyme activity during in vivo ischemia and its correlation to depressed myocardial function. J Biol Chem 260:208–214, 1985

    PubMed  CAS  Google Scholar 

  91. Zuurbier CJ, Beek JHGM van: The dynamic control of mitochondrial oxygen consumption in isolated perfused rabbit heart is not affected by a short period of ischaemia. J Physiol 479: 71–72P, 1994

    Google Scholar 

  92. Beek JHGM van, Hak JB, Wijhe MH van. Eijgelshoven MHJ, Westerhof N: The myocardial mitochondrial aerobic capacity is partially limiting for cardiac oxygen consumption and contractility. Europ J Physiol 430(S4): R9, 1995

    Article  Google Scholar 

  93. Groot B de, Dijk FJ, Beek JHGM van: Response of cardiac respiration to steps in heart rate is not slowed after reduction of mitochondrial capacity in normal and pressure overloaded hearts. Proceedings of the Congress of the International Union of Physiological Sciences, St. Petersburg, Russia, p. 063.06, 1997

    Google Scholar 

  94. JHGM van Beek, JB Hak, MH Wijhe van, N Westerhof: The control exerted by oxidative phosphorylation on respiration in the intact heart. In: HV Westerhoff, JL Snoep, JE Wijker, FE Sluse and BN Kholodenko (eds). Biothermokinetics of the Living Cell, BioThermoKinetics Press, Amsterdam, 1996, pp 119–123

    Google Scholar 

  95. Groot B de, Dijk FJ, Beek JHGM van: Limitation of contractile function and respiration by mitochondrial aerobic capacity in normal and pressure overloaded isolated rabbit hearts. Europ J Physiol, abstract 434: R45, 1997

    Google Scholar 

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Authors and Affiliations

  1. Laboratory for Physiology, Institute for Cardiovascular Research (ICaR-VU), Vrije Universiteit, Amsterdam, The Netherlands

    J. H. G. M. van Beek, C. J. Zuurbier, B. de Groot, M. H. J. Eijgelshoven & J. B. Hak

  2. Department of Pathophysiology, Datong Medical College, Datong, People’s Republic of China

    X. Tian

  3. Department of Experimental Cardiology, University Hospital Utrecht, The Netherlands

    C. J. A. van Echteld & M. H. J. Eijgelshoven

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  1. J. H. G. M. van Beek
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  2. X. Tian
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  3. C. J. Zuurbier
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  4. B. de Groot
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  5. C. J. A. van Echteld
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  6. M. H. J. Eijgelshoven
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Editors and Affiliations

  1. Laboratories of Bioenergetics, Institute of Chemical and Biological Physics, Tallinn, Estonia

    Valdur A. Saks

  2. Cellular and Molecular Cardiology, U 446 INSERM, Chatenay-Malabry, France

    Renée Ventura-Clapier

  3. Laboratory of Bioenergetics, Joseph Fourier University, Grenoble, France

    Valdur A. Saks , Xavier Leverve  & André Rossi ,  & 

  4. Laboratory of Bioenergetics, Institute of Biochemistry and Cellular Genetics CNRS, Bordeaux, France

    Michel Rigoulet

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van Beek, J.H.G.M. et al. (1998). The dynamic regulation of myocardial oxidative phosphorylation: Analysis of the response time of oxygen consumption. In: Saks, V.A., Ventura-Clapier, R., Leverve, X., Rossi, A., Rigoulet, M. (eds) Bioenergetics of the Cell: Quantitative Aspects. Developments in Molecular and Cellular Biochemistry, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5653-4_21

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