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Measurement of the Oxygenation Status of the Isolated Perfused Rat Heart Using Near Infrared Detection

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Oxygen Transport to Tissue XVII

Abstract

The oxygenation status of isolated hearts has often been measured using optical detection methods for the visible range of the spectrum (Tamura et al., 1989). To the best of our knowledge there are no reports on measurement of myoglobin oxygenation using detection methods in the near-infrared region of the spectrum. On the other hand, equipment for near-infrared measurements on tissue is widely available and is in some instances quite inexpensive. Therefore we investigated in the present study whether near-infrared (NIR) detection at two wavelengths provides a way to conveniently determine myoglobin oxygenation in an isolated organ as small as the saline-perfused rat heart.

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References

  • Beek, J.H.G.M. van, Loiselle, D.S, and Westerhof, N, 1992, Calculation of oxygen diffusion across the surface of isolated perfused hearts, Am. J. Physiol. 263: H1003–H1010.

    PubMed  Google Scholar 

  • Beek, J.H.G.M. van, and Westerhof, N., 1991, Response time of cardiac mitochondrial oxygen consumption to heart rate steps, Am. J. Physiol. 260: H613–H625.

    PubMed  Google Scholar 

  • Chance, B., 1965, The energy-linked reaction of calcium with mitochondria, J. Biol. Chem. 240: 2729–2748.

    PubMed  CAS  Google Scholar 

  • Chance, B., Dait, M.T., Zhang, C, Hamaoka, T., and Hagerman, F., 1992, Recovery of exercise-induced desaturation in the quadriceps muscles of elite competitive rowers, Am. J. Physiol. 262: C766–C775.

    PubMed  CAS  Google Scholar 

  • Chance, B., Nioka, S., Kent, J., McCully, K., Fountain, M., Greenfeld, R., and Holtom, G., 1988, Time-resolved spectroscopy of hemoglobin and myoglobin in resting and ischemic muscle, Anal. Biochem. 174:698–707.

    Article  PubMed  CAS  Google Scholar 

  • Chance, B., Zhuang, Z., Unah, C, Alter, C, and Lipton, L., 1993, Cognition-activated low-frequency modulation of light absorption in human brain, Proc. Natl. Acad. Sci. USA 90: 3770–3774.

    Article  PubMed  CAS  Google Scholar 

  • Eijgelshoven, M.H.J., Beek, J.H.G.M. van, Mottet, I., Nederhoff, M.G.J., Echteid, CJ.A. van, and Westerhof, N., 1994, Cardiac high-energy phosphates adapt faster than oxygen consumption to changes in heart rate, Circ. Res., in press, scheduled for 75(4), 9 pages.

    Google Scholar 

  • Kennedy, F.G., and Jones, D.P., 1986, Oxygen dependence of mitochondrial function in isolated rat cardiacmyocytes, Am. J. Physiol 250: C374–C383.

    PubMed  CAS  Google Scholar 

  • Tamura, M., Hazeki, O., Nioka, S., and Chance, B., 1989, In vivo study of tissue oxygen metabolism using optical and nuclear magnetic resonance spectroscopies, Annu. Rev. Physiol. 51:813–834.

    Article  PubMed  CAS  Google Scholar 

  • Tamura, M., Oshino, N., Chance, B., and Silver, I., 1978, Optical measurements of intracellular oxygen concentration of rat heart in vitro, Arch. Biochem. Biophys. 191: 8–22.

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Johannes H. G. M. van Beek .

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© 1996 Plenum Press, New York

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van Beek, J.H.G.M., Osbakken, M.D., Chance, B. (1996). Measurement of the Oxygenation Status of the Isolated Perfused Rat Heart Using Near Infrared Detection. In: Ince, C., Kesecioglu, J., Telci, L., Akpir, K. (eds) Oxygen Transport to Tissue XVII. Advances in Experimental Medicine and Biology, vol 388. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0333-6_18

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  • DOI: https://doi.org/10.1007/978-1-4613-0333-6_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-8002-3

  • Online ISBN: 978-1-4613-0333-6

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