Abstract
In this report, we show that expression of the Coronavirus IBV mRNAl is regulated by its 5’-UTR. Evidence presented domonstrates that the IBV sequence from nucleotide 1 to 1904 directs very inefficient synthesis of a product of approximately 43 kDa. Deletion of either the first 362 bp or the whole part of the 5’-UTR, however, dramatically increased the expression of the 43 kDa protein species. The mechanisms involved were investigated by two different approaches. Firstly, translation of the same construct in the presence of [3H]-leucine ruled out the possibility that initiation of small reading frames from non-AUG codons located in the 5’-UTR may compete with the authentic AUG initiation codon, and therefore inhibit the expression of ORF 1a. Secondly, expression and deletion analyses of a dicistronic construct showed that translation of the 43 kDa protein was initiated by ribosome internal entry mechanism. These studies suggest that a ‘weak’ ribosome internal entry signal is located in the 5’-UTR and is involved in the regulation of mRNA1 expression.
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Keywords
- Infectious Bronchitis Virus
- Polymerase Chain Reaction Fragment
- Avian Infectious Bronchitis Virus
- Downstream ORFs
- Inefficient Expression
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© 1998 Springer Science+Business Media New York
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Liu, D.X., Xu, H.Y., Lim, K.P. (1998). Regulation of mRNA 1 Expression by the 5’-Untranslated Region (5’-UTR) of the Coronavirus Infectious Bronchitis Virus (IBV). In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_40
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DOI: https://doi.org/10.1007/978-1-4615-5331-1_40
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