Abstract
Emphasis was placed in this short survey on recent chemical and biochemical aspects of oxidative base damage to DNA. Significant progress has been accomplished in a better understanding of the oxidation reactions mediated by OH radical, singlet oxygen and one-electron oxidation of the guanine moiety of DNA and model compounds. As a second major topic, relevant information was recently gained on the origin of major artifacts in the measurement (gas chromatography-mass spectrometry, high performane liquid chromatography-[32P]-postlabeling and immunological assays) of oxidative base lesions in cellular DNA. Prepurification of the targeted base damage prior to derivatization and enzymatic phosphorylation is a requisite to prevent artifactual oxidation to occur in the two former assays. Finally insight is provided on the specificity of repair by DNA-glycosylases using site specific modified oligonucleotides
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Cadet, J. et al. (1999). Oxidative Base Damage to DNA. In: Dizdaroglu, M., Karakaya, A.E. (eds) Advances in DNA Damage and Repair. NATO ASI Series, vol 302. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4865-2_5
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