Enhanced Eicosanoid Release in Ras-Transformed Cells
Our laboratory is studying the spectrum of ionic and biochemical events which are activated seconds to minutes after the addition of mitogens to quiescent fibroblasts. Previous work determined serum and bradykinin to rapidly stimulate phospholipases A2 and C in human fibroblasts (1–4). Activation of these phospholipases results in the production of a variety of lipid-derived “second messengers” (including arachidonate and its metabolites, diacylglycerol and inositol trisphosphate). These messengers stimulate a variety of cellular processes which are thought to be important components of the mitogenic response — including changes in cellular ion fluxes (2–8), activation of protein kinases (7,8), and induction of rapid transcriptional events (9,10).
KeywordsInositol Phosphate Inositol Trisphosphate Bradykinin Receptor Arachidonate Metabolite Rodent Fibroblast
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