Abstract
One of the unique developmental processes studied in plants is the induction and formation of nitrogen fixing root nodules on leguminous plants. Several plant genes encoding “nodulins” specifically involved in different stages of nodulation has been characterised (Listed in Delauney and Verma 1988). Early nodulin genes induced shortly after infection with the rhizobial microsymbiont respond to signal molecules excreted by the bacteria, while later nodulin genes like leghemoglobin responsible for oxygen transport in the mature nodule seem to require additional factors for induction (Govers et al. 1986/ Scheres et al. 1990). In an approach to study the regulatory circuits directing expression of nodulin genes we have used a Lotus corniculatus transformation regeneration system (Petit et al. 1987; Stougaard et al. 1987; Hansen et al. 1989) to define cis-acting regulatory DNA elements on both early and late nodulin promoters. In this communication results from analysis of the soybean leghemoglobin 1bc 3 gene promoter will be presented, together with attempts to characterise trans-acting protein factors linking the developmental status of the cell to regulatory events at the promoter.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Govers, F., Moerman, M., Downie, J.A., Hooykaas, P., Franssen, F.J., Louwerse, J., Van Kammen, A., and Bisseling, T., 1986, Rhizobium nod genes are involved in inducing an early nodulin gene. Nature, 323:564–566.
Hansen, J., Jorgensen J-E., Stougaard, J., and Marcker, K.A., 1989, Hairy roots — a short cut to transgenic root nodules. Plant Cell Rep., 8:12–15.
Jacobsen, K., Laursen N.B., Jensen E.O., Marcker, A., Poulsen, C., and Marcker, K.A., 1990, HMG I like proteins from leaf and nodule nuclei interact with different AT motifs in soybean nodulin promoters. The Plant Cell, 2:85–94.
Jensen, E.Ø., Marcker, K.A., Schell, J., and de Bruijn, J., 1988, Interaction of a nodule specific, trans-acting factor with distinct DNA elements in the soybean leghemoglobin 1bc 3 5′upstream region. EMBO J., 7:1265–1271.
Jorgensen, J-E., Stougaard J., Marcker, A., and Marcker, K.A., 1988, Root nodule specific gene regulation: Analysis of the soybean nodulin N23 gene promoter in heterologous symbiotic systems. Nucleic Acids Res. 16:39–50.
Petit, A., Stougaard J., Kühle, A., Marcker, K.A., and Tempe, J., 1987, Transformation and regeneration of the legume Lotus corniculatus: A system for molecular studies of symbiotic nitrogen fixation. Mol. Gen. Genet. 207:245–250.
Scheres, B., Van De Wiel, C., Zalanski, A., Horvath, B., Spaink, H., Van Eck, H., Zwartkruis, F., Wolters, A-M., Gloudemans, T., Van Kammen, A., and Bisseling, T., 1990, The ENOD12 product is involved in the infection process during the pea-Rhizobium interaction. Cell 60:281–294.
Stougaard, J., Marcker, K.A., Otten, L., and Schell, J., 1986, Nodule-specific expression of a chimeric soybean leghemoglobin gene in transgenic Lotus corniculatus. Nature 321:669–674.
Stougaard, J., Abildsten, D., and Marcker, K.A., 1987, The Agrobacterium rhizogenes pRi TL-segment as a gene vector system for transformation of plants. Mol. Gen. Genet. 207:251–255.
Stougaard, J., Sandal, N.N., Gron, A., Kühle, A., and Marcker, K.A., 1987, 5′analysis of the soybean leghemoglobin 1bc 3 gene: Regulatory elements required for promoter activity and organ specificity. EMBO J., 6:3565–3569.
Stougaard, J., Jorgensen, J-E., Christensen, T., Kühle, A., and Marcker, K.A., 1990, Interdependence and nodule specificity of cis-acting regulatory elements in the soybean leghemoglobin 1bc 3 and N23 promoters. Molec. Gen. Genet. 220:353–360.
Delauney, A.J., and Verma D.P.S., 1988, Cloned nodulin genes for symbiotic nitrogen fixation. P1. Mol.Biol. Rep. 6:279–285.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Lauridsen, P., Sandal, N., Kühle, A., Marcker, K., Stougaard, J. (1991). Regulation of Nodule Specific Genes. In: Herrmann, R.G., Larkins, B.A. (eds) Plant Molecular Biology 2. NATO ASI Series, vol 212. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3304-7_13
Download citation
DOI: https://doi.org/10.1007/978-1-4615-3304-7_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6454-2
Online ISBN: 978-1-4615-3304-7
eBook Packages: Springer Book Archive