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Nuclear Magnetic Resonance Studies of Thrombin-Fibrinopeptide and Thrombin-Hirudin Complexes

  • Chapter
Thrombin

Abstract

The thrombin-fibrinogen interaction to form the fibrin clot proceeds in three reversible steps (Scheraga and Laskowski, 1957), with thrombin being involved in only the first one:

  1. Step 1

    Proteolysis \({\text{F}}\,\overset {\text{T}} \leftrightarrows \,{\text{f + P}} \)

  2. Step 2

    Polymerization \(nf \rightleftarrows {f_n} \)

  3. Step 3

    Clotting \(m{{\text{f}}_n} \rightleftarrows {\text{fibrin}} \)

where F is fibrinogen, T is thrombin, f is the fibrin monomer, P represents the fibrinopeptides A and B (two each) released by proteolytic cleavage of the Arg—Gly bonds in the Aα and Bβ chains, respectively, of fibrinogen, fn is a series of intermediate, rodlike staggered overlapped polymers of variable length (i.e., varying n), and fibrin is the clotted product formed by the incorporation of m rodlike polymers in an organized networklike structure.

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Author information

Authors and Affiliations

  1. Biotechnology Research Institute, National Research Council of Canada, Montréal, Québec, Canada, H4P 2R2

    Feng Ni

  2. Baker Laboratory of Chemistry, Cornell University, Ithaca, New York, 14853-1301, USA

    Kenneth D. Gibson & Harold A. Scheraga

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  1. Feng Ni
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  2. Kenneth D. Gibson
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  3. Harold A. Scheraga
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Editors and Affiliations

  1. The Ohio State University, Columbus, Ohio, USA

    Lawrence J. Berliner

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Ni, F., Gibson, K.D., Scheraga, H.A. (1992). Nuclear Magnetic Resonance Studies of Thrombin-Fibrinopeptide and Thrombin-Hirudin Complexes. In: Berliner, L.J. (eds) Thrombin. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3296-5_2

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  • DOI: https://doi.org/10.1007/978-1-4615-3296-5_2

  • Publisher Name: Springer, Boston, MA

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