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Enzymology of ADP-ribose polymer synthesis

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ADP-Ribosylation: Metabolic Effects and Regulatory Functions

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 12))

Abstract

In this minireview, we summarize recent advances on the enzymology of ADP-ribose polymer synthesis. First, a short discussion of the primary structure and cloning of poly(ADP-ribose) polymerase (PARP) [EC 2.4.2.30], the enzyme that catalyzes the synthesis of poly(ADP-ribose), is presented. A catalytic distinction between the multiple enzymatic activities of PARP is established. The direction of ADP-ribose chain growth as well as the molecular mechanism of the automodification reaction catalyzed by PARP are described. Current approaches to dissect ADP-ribose polymer synthesis into individual reactions of initiation, elongation and branching, as well as a partial mechanistic characterization of the ADP-ribose elongation reaction at he chemical level are also presented. Finally, recent developments in the catalytic characterization of PARP by site-directed mutagenesis are also briefly summarized. (Mol Cell Biochem 138: 33–37, 1994)

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Authors and Affiliations

  1. The Departments of Microbiology & Immunology, University of North Texas Health Science Center at Fort Worth, Fort Worth, Texas, 76107-2699, USA

    Rafael Alvarez-Gonzalez & Hilda Mendoza-Alvarez

  2. The Departments of Biochemistry & Molecular Biology, University of North Texas Health Science Center at Fort Worth, Fort Worth, Texas, 76107-2699, USA

    Rafael Alvarez-Gonzalez, Gustavo Pacheco-Rodriguez & Hilda Mendoza-Alvarez

Authors
  1. Rafael Alvarez-Gonzalez
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  2. Gustavo Pacheco-Rodriguez
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  3. Hilda Mendoza-Alvarez
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Editors and Affiliations

  1. Laboratory of Cellular Metabolism, National Heart, Lung and Blood Institute, NIH, Building 10, Room 5N-307, Bethesda, MD, 20892, USA

    Joel Moss

  2. Division of Cardiovascular Sciences, St. Boniface General Hospital Research Center, 351 Tache Avenue, Winnipeg, MB, R2H 2A6, Canada

    Peter Zahradka

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Alvarez-Gonzalez, R., Pacheco-Rodriguez, G., Mendoza-Alvarez, H. (1994). Enzymology of ADP-ribose polymer synthesis. In: Moss, J., Zahradka, P. (eds) ADP-Ribosylation: Metabolic Effects and Regulatory Functions. Developments in Molecular and Cellular Biochemistry, vol 12. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2614-8_4

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  • DOI: https://doi.org/10.1007/978-1-4615-2614-8_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6120-6

  • Online ISBN: 978-1-4615-2614-8

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