Abstract
K+ channels represent a large and diverse group of ion channels that play a fundamental role in controlling cell excitability. These channels regulate hormone release from endocrine cells, modulate the pattern of transmitter release from neurons, and set the level of contraction in arterial smooth muscle cells. At first glance, K+ channels can be subdivided into either voltage-gated K+ (Kv) channels or ligand-gated K+ channels, depending on the stimulus that triggers the conformational change that leads to channel opening. Within the wide family of K+ channels gated by voltage, the Kv channels are activated solely by membrane depolarization, whereas high-conductance Ca2+-activated K+ (BKCa) channels require both membrane depolarization and an increased level of cytosolic free Ca2+ ([Ca2+]i) to activate effectively. The use of molecular biology techniques has greatly extended our current understanding regarding the structure and existence of subfamilies of K+ channels. However, three major questions still are the subject of extensive investigation: (1) What is the molecular composition of the different types of K+ channels expressed in vivo, (2) what is the physiological function of these K+ channels, and (3) is the molecular composition and functional profile of K+ channels altered in pathophysiological states?
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References
Berger, M. G., Rusch, N. J. 1999, Voltage and calcium-gated potassium channels: Functional expression and therapeutic potential in the vasculature, In: Perspectives in Drug Discovery and Design, Y. C. Martin (series ed.), vol. 15/16 Kluwer Academic Plenum Publishers, Dordrecht, The Netherlands, pp. 313–332.
Crest, M., Jacquet, G., Gola, M., Zerrouk, H., Benslimane, A., Rochat, H., Mansuelle P., and Martin- Eauclaire, M. F., 1992, Kaliotoxin, a novel peptidyl inhibitor of neuronal BK-type Ca2+-activated K+ channels characterized from Androctonus mauretanicus mauretanicus venom, J. Biol. Chem. 267:1640– 1647.
Darbon H., Blanc, E. and Sabatier, J.-M., 1999, Three-dimensional structure of scorpion toyins: Towards a new model of interaction with potassium channels, In: Perspectives in Drug Discovery and Design (Y. C. Martin, Series ed.), Vol. 15/16, Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 41–60.
Galvez, A., Gimenez-Gallego, G., Reuben, J. P., Roy-Contancin, L., Feigenbaum, P., Kaczorowski, G. J., and Garcia, M. L., 1990, Purification and characterization of a unique, potent, peptidyl probe for the high conductance calcium-activated potassium channel from venom of the scorpion Buthus tamulus, J. Biol. Chem. 265:11083–11090.
Garcia, M. L., Garcia-Calvo, M., Hidalgo, P., Lee, A., and MacKinnon, R., 1994, Purification and characterization of three inhibitors of voltage-dependent K+ channels from Leiurus quinquestriatus var. hebraeus venom, Biochemistry 33:6834–6839.
Garcia-Calvo, M., Leonard, R. J., Novick, J., Stevens, S. P., Schmalhofer, W., Kaczorowski, G. J., and Garcia, M. L., 1993, Purification, characterization, and biosynthesis of margatoxin, a component of Centruroides margaritatus venom that selectively inhibits voltage-dependent potassium channels, J. Biol. Chem. 268:18866–18874.
Knaus, H. -G., Eberhart, A., Koch, R. O. A., Munujos, P., Schmalhofer, W. A., Warmke, J. W., Kaczorowski, G. J., and Garcia, M. L., 1995, Characterization of tissue-expressed α subunits of the high conductance Ca2+activated K+ channel, J. Biol Chem.270:22434–22439.
Koch, R. O., Wanner, S. G., Koschak, A., Hanner, M., Schwarzer, C., Kaczorowski, G. J., Slaughter, R. S., Garcia, M. L., and Knaus, H. G., 1997, Complex subunit assembly of neuronal voltage-gated K+ channels: Basis for high-affinity toxin interactions and pharmacology, J. Biol. Chem. 272:27577–27581.
Koschak, A., Bugianesi, R. M., Mitterdorfer, J., Kaczorowski, G. J., Garcia, M. L. and Knaus, H. G., 1998, Subunit composition of brain voltage-gated potassium channels determined by hongotoxin-1, a novel peptide derived from Centruroides limbatus venom, J. Biol. Chem. 273:2639–2644.
Liu, Y., Pleyte, K. A., Knaus, H.-G., and Rusch, N. J., 1997, Increased expression of Ca2+sensitive K+ channels in aorta of hypertensive rats, Hypertension 30:1403–1409.
Liu, Y., Hudetz, A. G., Knaus, H.-G., and Rusch, N. J., 1998, Increased expression of Ca2+sensitive K+ channels in the cerebral microcirculation of genetically hypertensive rats: Evidence for protection against cerebral vasospasm, Circ. Res. 82:729–737.
McCobb, D. P., Fowler, N. L., Featherstone, T., Lingle, C. J., Saito, M., Krause, J. E., and Salkoff, L., 1995, A human calcium-activated potassium channel gene expressed in vascular smooth muscle,Am. J. Physiol. 269:H767–H777.
Overturf, K. E., Russell, S. N., Carl, A., Vogalis, F., Hart, P. J., Hume, J. R., Sanders, K. M., and Horowitz, B., 1994, Cloning and characterization of a Kvl.5 delayed rectifier K+ channel from vascular and visceral smooth muscles, Am. J. Physiol. 267:C1231–C1238.
Parcej, D. N., and Dolly, J. O., 1989, Dendrotoxin acceptor from bovine synaptic plasma membranes: Binding properties, purification and subunit composition of a putative constituent of certain voltage-activated K+ channels [see comments], Biochem. J. 257:899–903.
Rehm, H., and Lazdunski, M., 1988, Purification and subunit structure of a putative K+-channel protein identified by its binding properties for dendrotoxin I, Proc. Natl. Acad. Sci. U.S.A. 85:4919–4923.
Roberds, S. L., and Tamkun, M. M., 1991, Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in the rat heart,Proc. Natl. Acad. Sci. U.S.A. 88:1798–1802.
Roeper, J., Sewing, S., Zhang, Y., Sommer, T., Wanner, S. G., and Pongs, O., 1998, NIP domain prevents N-type inactivation in voltage-gated potassium channels, Nature 391:390–393.
Shamotienko, O. G., Parcej, D. N., and Dolly, J. O.,1997, Subunit combinations defined for K+ channel Kv1 subtypes in synaptic membranes from bovine brain, Biochemistry 36:8195–8201.
Sheng, M., Liao, Y. J., Jan, Y. N., and Jan, L. Y., 1993, Presynaptic A-current based on heteromultimeric K+ channels detected in vivo, Nature 365:72–75.
Sheng, M., Tsaur, M. L., Jan, Y. N., and Jan, L. Y., 1994, Contrasting subcellular localization of the Kv1.2 K+ channel subunit in different neurons of rat brain, J. Neurosci. 14:2408–2417.
Stuhmer, W., Ruppersberg, J. P., Shroter, K. H., Sakmann, B., Stocker, M., Giese, K. P., Perschke, A., Baumann, A., and Pongs, O., 1989, Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain, EMBO J. 8:3235–3244.
Vazquez, J., Feigenbaum, P., Katz, G., King, V. F., Reuben, J. P., Roy-Contancin, L., Slaughter, R. S., Kaczorowski, G. J., and Garcia, M. L., 1989, Characterization of high affinity binding sites for charybdotoxin in sarcolemmal membranes from bovine aortic smooth muscle: Evidence for a direct association with the high conductance calcium-activated potassium channel, J. Biol. Chem. 64:20902– 20909.
Vazquez, J., Feigenbaum, P., King, V. F., Kaczorowski, G. J., and Garcia, M. L., 1990, Characterization of high affinity binding sites for charybdotoxin in synaptic plasma membranes from rat brain: Evidence for a direct association with an inactivating, voltage-dependent, potassium channel, J. Biol. Chem. 265:15564–15571.
Veh, R. W., Lichtinghagen, R., Sewing, S., Wunder, F., Grumbach, I. M., and Pongs, O., 1995, Immunohistochemical localization of five members of the Kv1 channel subunits: Contrasting subcellular locations and neuron-specific co-localizations in rat brain,Eur. J. Neurosci. 7:2189–2205.
Wang, H., Kunkel, D. D., Schwartzkroin, P. A., and Tempel, B. L, 1994, Localization of Kv1.1 and Kv1.2, two K channel proteins, to synaptic terminals, somata, and dendrites in the mouse brain, J. Neurosci. 14:4588–4599.
Wang, J., Juhaszova, J., Rubin, L. J., and Yuan, X,-J., 1997, Hypoxia inhibits gene expression of voltage-gated K+ channels α-subunits in pulmonary artery smooth muscle cells, J. Clin. Invest. 100:2347–2353.
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Koch, R.O. et al. (2001). Design and Use of Antibodies for Mapping K+Channel Expression in the Cardiovascular System. In: Archer, S.L., Rusch, N.J. (eds) Potassium Channels in Cardiovascular Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1303-2_6
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DOI: https://doi.org/10.1007/978-1-4615-1303-2_6
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