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Wheat Catalase Expressed in Transgenic Rice Plants Can Improve Tolerance Against Low Temperature Injury

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Plant Cold Hardiness

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Abstract

We isolated wheat catalase (CAT: EC 1.11.1.6) cDNA and introduced it into rice (Oryza sativa L.). Some of 56 transgenic regenerated rice plants were confirmed to express the wheat catalase by the native PAGE with catalase activity staining. The wheat catalase detected in leaf, root, anther, seed germ and seed endosperm suggested that wheat catalase under 35S CaMV promoter is expressed in these tissues. In the transgenic rice plants, the catalase activities in leaf at 25 °C and at 5 °C were increased from 2 to 5 fold and 4 to 15 fold respectively, compared to that of non-transgenic rice. The transgenic rice CT 2-6-4, which showed the highest catalase activity among the transgenic rice plants indicated an increased resistance to low temperature stress. These results were obtained by comparing the damage in leaves of withering (curling) due to chilling at 5 °C. This chilling treatment resulted in the decrease of catalase activities in both types of plant, but the transgenic plants indicated higher catalase activities retained in the leaves than those of non-transgenic ones. The transgenic CT 2-6-4 also contained lower concentration of hydrogen peroxide in leaves than the control rice plant. During the chilling, the H2O2 concentration of non-transgenic rice increased, but the increase was depressed in the transgenic rice. Therefore, enhanced activities of catalase in genetically engineered rice plants lead to the effective detoxification of H2O2, which improves tolerance against low temperature injury.

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Authors and Affiliations

  1. Research Institute of Biological Resources, National Institute of Advanced Industrial and Science Technology, 2-17-1, 2, Tsukisamuhigashi, Toyohira-ku, Sapporo, 062-8517, Japan

    Takeshi Matsumuraa

  2. Hokkai Sankyo Co., Ltd., 27-4, Kitanosato, Kitahiroshima, Hokkaido, 061-1111, Japan

    Noriko Tabayashib

  3. Hokkaido Green-Bio Institute, Higashi 5 Kita 15, Naganuma Hokkaido, 069-1301, Japan

    Yasuyo Kamagata & Chihiro Souma

  4. Hokkaido Green-Bio Institute, Higashi 5 Kita 15, Yubari-gun, Naganuma, Hokkaido, 069-1301, Japan

    Haruo Saruyama

Authors
  1. Takeshi Matsumuraa
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  2. Noriko Tabayashib
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  3. Yasuyo Kamagata
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  4. Chihiro Souma
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  5. Haruo Saruyama
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Editors and Affiliations

  1. University of Minnesota, St. Paul, Minnesota, USA

    Paul H. Li

  2. University of Helsinki, Helsinki, Finland

    E. Tapio Palva

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Matsumuraa, T., Tabayashib, N., Kamagata, Y., Souma, C., Saruyama, H. (2002). Wheat Catalase Expressed in Transgenic Rice Plants Can Improve Tolerance Against Low Temperature Injury. In: Li, P.H., Palva, E.T. (eds) Plant Cold Hardiness. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0711-6_20

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  • DOI: https://doi.org/10.1007/978-1-4615-0711-6_20

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5205-1

  • Online ISBN: 978-1-4615-0711-6

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