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In Vivo Imaging of MSCs

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Mesenchymal Stromal Cells

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

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Abstract

Clinical applications of mesenchymal stromal/stem cells (MSCs) to treat a myriad of diseases are currently being initiated. For MSC therapy to become common practice, basic questions about the fate of MSCs once administered need to be addressed. These include the biodistribution, survival, and differentiation of MSCs. In this chapter, several imaging techniques are described that can be used to interrogate these questions in a clinically applicable manner, and several examples are discussed. Single-photon emission computed tomography (SPECT)/radioisotope imaging and magnetic resonance imaging (MRI) cell-tracking techniques are the two modalities that likely will become mainstays in clinical practice.

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Abbreviations

BLI:

Bioluminescent imaging

FDA:

Food and Drug Administration

FHBG:

9-(4-[18F] Fluoro-3-hydroxymethylbutyl) guanine

FIAU:

1-(2-deoxy-2-fluoro-1-d-arabinofuranosyl)-5-iodouracil

GFP:

Green fluorescent protein

HSV-tk:

Herpes simplex virus thymidine kinase

MI:

Myocardial infarct

MSC:

Mesenchymal stem cell

MRI:

Magnetic resonance imaging

PET:

Positron emission tomography

SPECT:

Single-photon emission computed tomography

SPIO:

Superparamagnetic iron oxide

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Acknowledgment

The author is supported by grant MSCRFII-0161-00.

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Authors and Affiliations

  1. Division of MR Research and Cellular Imaging Section, Russell H. Morgan Department of Radiology and Radiological Science, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205-2195, USA

    Jeff W. M. Bulte Ph.D.

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  1. Jeff W. M. Bulte Ph.D.
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Correspondence to Jeff W. M. Bulte Ph.D. .

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Editors and Affiliations

  1. Madison School of Medicine and Public He, Department of Medicine, Hematology/Oncol, University of Wisconsin, 1111 Highland Avenue MC2275, Madison, 53705, USA

    Peiman Hematti

  2. Princess Margaret Hospital, Cell Therapy Program, University of Toronto, University Avenue 610, Toronto, M5G 2M9, Ontario, Canada

    Armand Keating

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Bulte, J.W.M. (2013). In Vivo Imaging of MSCs. In: Hematti, P., Keating, A. (eds) Mesenchymal Stromal Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-5711-4_21

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