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High-Throughput Biophysical Approaches to Therapeutic Protein Development

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Biophysics for Therapeutic Protein Development

Part of the book series: Biophysics for the Life Sciences ((BIOPHYS,volume 4))

Abstract

High-throughput methods have become indispensible tools in the development of proteins as therapeutic agents and are being applied in every phase of protein product development from candidate selection and purification development through commercial formulation development. This chapter presents some of the latest refinements in high-throughput biophysical methods and their application in therapeutic protein development. The methods discussed include liquid chromatography, surface plasmon resonance, light absorption spectroscopy, vibrational spectroscopy, fluorescence spectroscopy, calorimetry (both isothermal titration and differential scanning), and light scattering. In addition, empirical phase diagram as a tool to interpret results from high-throughput biophysical approaches is discussed. Finally, an industry perspective on the advantages and challenges of implementing high-throughput technology in therapeutic protein development is presented.

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Authors and Affiliations

  1. Drug Product Development, Amgen Inc., 1201 Amgen Court West, Seattle, WA, 98119, USA

    Feng He, Vladimir I. Razinkov & Gerald W. Becker

  2. Department of Pharmaceutical Chemistry, University of Kansas, 2030 Becker Dr., Lawrence, KS, 66047, USA

    C. Russell Middaugh

Authors
  1. Feng He
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  2. Vladimir I. Razinkov
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  3. C. Russell Middaugh
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  4. Gerald W. Becker
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Corresponding author

Correspondence to Feng He .

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Editors and Affiliations

  1. , Research & Development, Amgen, Inc., One Amgen Center Drive, Thousand Oaks, 91320, California, USA

    Linda O. Narhi

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He, F., Razinkov, V.I., Middaugh, C.R., Becker, G.W. (2013). High-Throughput Biophysical Approaches to Therapeutic Protein Development. In: Narhi, L. (eds) Biophysics for Therapeutic Protein Development. Biophysics for the Life Sciences, vol 4. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-4316-2_2

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