Biophysics for Therapeutic Protein Development

  • Linda O.┬áNarhi

Part of the Biophysics for the Life Sciences book series (BIOPHYS, volume 4)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Linda O. Narhi
    Pages 1-5
  3. Feng He, Vladimir I. Razinkov, C. Russell Middaugh, Gerald W. Becker
    Pages 7-31
  4. James Kranz, Fatma AlAzzam, Atul Saluja, Juraj Svitel, Wasfi Al-Azzam
    Pages 33-82
  5. Yijia Jiang, Cynthia Li, John Gabrielson
    Pages 99-126
  6. Satish K. Singh, Qin Zou, Min Huang, Muralidhara Bilikallahalli
    Pages 147-172
  7. Sreedhara Alavattam, Barthelemy Demeule, Jun Liu, Sandeep Yadav, Mary Cromwell, Steven J. Shire
    Pages 173-204
  8. Tingting Wang, Sangeeta B. Joshi, Ozan S. Kumru, Srivalli Telikepalli, C. Russell Middaugh, David B. Volkin
    Pages 205-243
  9. Zai-Qing Wen, Gianni Torraca, Guiyang Li, Xiaolin Cao, Chanel K. Yee
    Pages 245-260
  10. Evi B. Struble, John F. Cipollo, Chava Kimchi-Sarfaty, Zuben E. Sauna, Jack A. Ragheb, Ewa Marszal
    Pages 261-281
  11. Back Matter
    Pages 283-293

About this book


The last few decades have seen the genesis of protein therapeutics, such that these large molecule based drugs comprise an increasingly larger part of the commercial market. The conformation (overall global fold or three-dimensional structure) of these molecules is important for maintaining biological activity, stability during long term storage, and can impact the safety profile and biological consequences of administration. For this reason at every stage of product discovery and development biophysical methods play a key analytical role in product development, as they are applied for the determination of protein higher order structure. In addition to helping define binding kinetics and other parameters important for target validation, they are the only techniques available to determine if a potential protein product is folded properly, and can maintain this active conformation during manufacturing, storage, and delivery. Thus the biophysical techniques play a key role during the development of protein therapeutics.

This volume is organized to mimic the product lifecycle. The initial chapters describe the underlying theory, and strengths and weaknesses of the different techniques commonly used during therapeutic development. The majority of the chapters discuss the applications of these techniques, including case studies, across the product lifecycle from early discovery, where the focus is on identifying targets, and screening for potential drug product candidates, through expression and purification, large scale production, formulation development, lot-to-lot comparability studies, and commercial support including investigations. There is also a chapter from the perspective of the regulatory agencies. This book can be used to provide insight into this important application of biophysics for those who are planning a career in protein therapeutic development, and for those outside this area who are interested in understanding it better.



biopharmaceuticals biophysics biotherapeutics lifecycle protein aggregation

Editors and affiliations

  • Linda O.┬áNarhi
    • 1
  1. 1., Research & DevelopmentAmgen, Inc.Thousand OaksUSA

Bibliographic information