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Applications of Mass Spectrometry in Analyses of Steroid Hormones

  • Zimeng YanEmail author
  • Chang Cheng
  • Shaorong Liu
Chapter

Abstract

Steroid hormones are endogenous chemicals controlling many endocrinology functions. Mass spectrometry technologies have been applied for analyses of steroid hormones as biomarkers in endocrinology and pathology diagnoses, doping drugs in athletes and racing horses, residuals in food safety concerns, and environmental pollutants in water and sediments. Both liquid chromatography mass spectrometry (LC-MS or LC-MS/MS) and gas chromatography mass spectrometry (GC-MS or GC-MS/MS) are broadly used in research, clinical, pharmaceutical industry, competition sports, food safety, and environmental testing laboratories. Sample preparation techniques, such as deconjugation and extraction, are critical procedures for isolating steroid hormone from sample matrices, including biological fluids, tissues, environmental water and sediments. Chemical derivatization modifies the physicochemical properties of steroid hormone molecules to improve their chromatographic performances and to enhance their sensitivities to mass detection. Chromatographic techniques such as HPLC, UPLC, and GC have direct impact on separation of analytes, MS interface, and analysis throughput. The method sensitivity and specificity of LC-MS and GC-MS depend largely on the analyte status, i.e., easiness of ionization, derivatization, sample matrix, and MS detection mode, e.g., ESI, APCI, APPI, MAILDI, or EI. LC-MS and GC-MS methodologies should be developed and validated following scientific and regulatory guidelines, and the steroid hormones analyses should be standardized.

Keywords

Atmospheric Pressure Chemical Ionization Supercritical Fluid Chromatography Derivatization Reagent Helix Pomatia Sulfonyl Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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International Organization of Standardization, Guide:

  1. ISO Guide 30:1992 Terms and definitions used in connection with reference materialsGoogle Scholar
  2. ISO Guide 30:1992/Amd 1:2008 Revision of definitions for reference material and certified reference materialGoogle Scholar
  3. ISO Guide 31:2000 Reference materials—contents of certificates and labelsGoogle Scholar
  4. ISO Guide 32:1997 Calibration in analytical chemistry and use of certified reference materialsGoogle Scholar
  5. ISO Guide 33:2000 Uses of certified reference materialsGoogle Scholar
  6. ISO Guide 34:2009 General requirements for the competence of reference material producersGoogle Scholar
  7. ISO Guide 35:2006 Reference materials—general and statistical principles for certification. http://www.iso.org/iso/search.htm?qt=guide+35&searchSubmit=Search&sort=rel&type=simple&published=on

International Organization of Standardization, Published:

  1. ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results—Part 1: general principles and definitionsGoogle Scholar
  2. ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results—Part 2: basic method for the determination of repeatability and reproducibility of a standard measurement methodGoogle Scholar
  3. ISO 5725-3:1994 Accuracy (trueness and precision) of measurement methods and results—Part 3: intermediate measures of the precision of a standard measurement methodGoogle Scholar
  4. ISO 5725-4:1994 Accuracy (trueness and precision) of measurement methods and results—Part 4: basic methods for the determination of the trueness of a standard measurement methodGoogle Scholar
  5. ISO 5725-5:1998 Accuracy (trueness and precision) of measurement methods and results—Part 5: alternative methods for the determination of the precision of a standard measurement methodGoogle Scholar
  6. ISO 5725-6:1994 Accuracy (trueness and precision) of measurement methods and results—Part 6: use in practice of accuracy values. http://www.iso.org/iso/search.htm?qt=5725&published=on&active_tab=standards

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Research Pharmaceutical Services, Inc.Fort WashingtonUSA
  2. 2.Analytical DevelopmentAlbany Molecular Research, Inc.RensselaerUSA
  3. 3.Department of Chemistry and BiochemistryUniversity of OklahomaNormanUSA

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