Abstract
Over the past several years, our laboratory has been studying the mechanism and regulation of biological alkyl transfer reactions. Our approach has been to combine chemical and biological techniques in order to answer questions concerning the means by which these enzyme-mediated processes are catalyzed and controlled in living cells. Reviews of the chemical (1) and biological aspects (2) have been published, and the present paper will deal only with more recent results of our chemical mechanism studies. Ultimately, we would like to use this information in the design of potent, specific multisubstrate adduct (“transition state analog”) inhibitors (3) of these reactions.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
J.K. Coward and P.A. Crooks, in Transmethylation11, E. Usdin, et al., eds., Elsevier/North Holland, N.Y. (1979), p. 215.
J.K. Coward and P.A. Crooks, in Transmethylation11, E. Usdin, et al., eds., Elsevier/North Holland, N.Y. (1979), p. 215.
R. Wolfenden, Ann. Rev. Biophys. Bioeng., 5: 271 (1976).
J.M. Saavedra, Essays Neurochem. Neuropharmacol., 1: 1 (1977).
J.K. Coward, E.P. Slisz, and F. Y-H. Wu, Biochem, 12: 2291 (1973).
W.W. Cleland, Enzymes, 3rd Ed., 2:1 (1970).
R.W. Woodard, P.A. Crooks, J.K. Coward, and H.G. Floss, manuscript in preparation; c.f. H.G. Floss, et al., Ref. 1, p. 135.
R.T. Borchardt, J. Med. Chem., 16: 377 (1973).
S.J. Kerr, Methods Cancer Res, 15: 163 (1978).
J.M. Glick and P.S. Leboy, J. Biol. Chem., 252:4790 (1977); b. J.M. Glick, V.M. Averyhart and P.S. Leboy, Biochem. Biophys. Acta, 518: 158 (1978).
E. Barbosa and B. Moss, J. Biol. Chern., 253: 7692 (1978).
W.K. Paik and S. Kin, Adv. Eng., 42: 227 (1975).
M. Jamaluddin, S. Kim and W.K. Paik, Biochemistry, 14: 694, (1978).
A. Thithapandha and V.H. Cohn, Biochem. Pharmacol., 27: 263 (1978).
J.O. Knipe and J.K. Coward, J. Amer. Chem. Soc., 101:July, (1979).
J.O. Knipe, P.J. Vasquez, and J.K. Coward, manuscript in preparation.
T.C. Bruice and S.J. Benkovic, “Bioorganic Mechanisms”, W.A. Benjamin, N.Y. (1966), Vol. 1, p. 119; b. W.P. Jencks, “Catalysis in Chemistry and Enzymology”, McGraw-Hill, N.Y. (1969), p. 11.
T.C. Bruice, Ann. Rev. Biochem., 45:331 (1976); b. W.P. Jencks, Adv. Enz., 43: 219 (1975).
J.K. Coward and W.D. Sweet, J. Org. Chem., 36: 2337 (1971).
I. Mihel, J.O. Knipe, J.K. Coward, and R.L. Schowen, J. Amer. Chem. Soc., 101:July (1979).
M.F. Hegazi, R.T. Borchardt, and R.L. Schowen, J. Amer. Chem. Soc., 101:July (1979).
C.H. Gray, J.K. Coward, K.B. Schowen, and R.L. Schowen, J. Amer. Chem. Soc., 101:July (1979).
C.K. Ingold, Structure and Mechanism in Organic Chemistry11, 2nd Ed., Cornell University Press, N.Y. (1969) p. 457.
J.K. Coward, unpublished results.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1980 Plenum Press, New York
About this chapter
Cite this chapter
Coward, J.K. (1980). Methylase Mechanisms: Steric Constraints and Modes of Catalysis. In: Cavallini, D., Gaull, G.E., Zappia, V. (eds) Natural Sulfur Compounds. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3045-5_2
Download citation
DOI: https://doi.org/10.1007/978-1-4613-3045-5_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-3047-9
Online ISBN: 978-1-4613-3045-5
eBook Packages: Springer Book Archive