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Experimental Approaches for the Detection of Chromosomal Malsegregation Occurring in the Germline of Mammals

  • Chapter
Aneuploidy

Part of the book series: Basic Life Sciences ((BLSC,volume 36))

Abstract

Existing and newly proposed methods to detect the induction of heritable aneuploidy are summarized, and their favorable and unfavorable features discussed. Among the tests involving direct chromosomal examination, those involving study of pronuclear chromosomes at first cleavage are judged to be the most universally informative and reliable, provided tertiary trisomy can be ruled out. Measurement of postmidterm (fetal) death is proposed as a trisomy prescreen that can be readily combined with a dominant-lethal test. Among the genetic procedures for identifying the results of malsegregation events, direct detection of aneuploids has a number of advantages over complementation methods, in which only a fraction of the products of aneuploid gametes is detectable. Direct detection of aneuploids must, however, be restricted to sex chromosomes if postnatal animals are examined. A genetic marker system to detect autosomal trisomies in fetuses is proposed. An examination of experimental parameters that might maximize induction of malsegregation leads to the recommendation to include preleptotene among exposed germ-cell stages.

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Author information

Authors and Affiliations

  1. Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, 37831, USA

    Liane B. Russell

Authors
  1. Liane B. Russell
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Editor information

Editors and Affiliations

  1. U.S. Environment Protection Agency, Washington, D.C, USA

    Vicki L. Dellarco  & Peter E. Voytek  & 

  2. Council for Research Planning in Biological Sciences, Inc., Washington,D.C, USA

    Alexander Hollaender

  3. University of Alabama, Birmingham, Alabama, USA

    B. R. Brinkley

  4. New York State Department of Health, Albany, New York, USA

    Ernest B. Hook

  5. Duke University School of Medicine, Durham, North Carolina, USA

    Montrose J. Moses

  6. National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA

    Frederick J. de Serres

  7. M. D. Anderson Hospital and Tumor Institute, Houston, Texas, USA

    T. C. Hsu

  8. Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

    Liane B. Russell

  9. Brookhaven National Laboratory, Upton, New York, USA

    Raymond R. Tice

  10. U.S. Environmental Protection Agency Research Triagle Park, North Carolina, USA

    Michael D. Waters

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© 1985 Plenum Press, New York

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Russell, L.B. (1985). Experimental Approaches for the Detection of Chromosomal Malsegregation Occurring in the Germline of Mammals. In: Dellarco, V.L., et al. Aneuploidy. Basic Life Sciences, vol 36. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2127-9_26

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  • DOI: https://doi.org/10.1007/978-1-4613-2127-9_26

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9257-9

  • Online ISBN: 978-1-4613-2127-9

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