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The Anaerobic Stress Response and its Use for Studying Gene Expression in Conifers

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Genetic Manipulation of Woody Plants

Part of the book series: Basic Life Sciences ((BLSC,volume 44))

Abstract

For many angiosperms, a shift from an aerobic to an anaerobic environment elicits a stress response that involves increased transcription of specific genes. The best known of these genes are those encoding ADH. Adh genes have now been cloned from several angiosperm species and their regulation is being studied. Because of the available information for angiosperms, similar studies in conifers should provide insights into gene regulation in this evolutionarily distinct, commercially important group of woody plants. We have begun our studies using ADH from Pinus radiata. Induction of one of two ADH isozymes can be detected on starch gels within 12 hr after exposure of germinating seedlings to anaerobic conditions. We constructed a cDNA library in the phage vector λgt10 and screened this library using an Adh cDNA clone from corn as a probe. Several clones have been isolated and are being characterized by restriction analysis, filter hybridization, and DNA sequence analysis. Preliminary results show about 70% sequence homology between the pine cDNA clones and the corn Adh cDNA.

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Author information

Author notes

  1. David E. Harry

    Present address: Department of Forestry, University of Illinois, Urbana, Illinois, 61801, USA

  2. Ronald R. Sederoff

    Present address: Department of Forestry, North Carolina State University, Raleigh, North Carolina, 27695, USA

Authors and Affiliations

  1. Institute of Forest Genetics, Pacific Southwest Forest and Range Experiment Station, U.S. Department of Agriculture Forest Service, Berkeley, California, 94701, USA

    David E. Harry, Claire S. Kinlaw & Ronald R. Sederoff

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  1. David E. Harry
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  2. Claire S. Kinlaw
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  3. Ronald R. Sederoff
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Editors and Affiliations

  1. Michigan State University, East Lansing, Michigan, USA

    James W. Hanover  & Daniel E. Keathley  & 

  2. Council for Research Planning in Biological Sciences, Inc., Washington, D.C., USA

    Claire M. Wilson  & Gregory Kuny  & 

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© 1988 Plenum Press, New York

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Harry, D.E., Kinlaw, C.S., Sederoff, R.R. (1988). The Anaerobic Stress Response and its Use for Studying Gene Expression in Conifers. In: Hanover, J.W., Keathley, D.E., Wilson, C.M., Kuny, G. (eds) Genetic Manipulation of Woody Plants. Basic Life Sciences, vol 44. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1661-9_18

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  • DOI: https://doi.org/10.1007/978-1-4613-1661-9_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8922-7

  • Online ISBN: 978-1-4613-1661-9

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