Abstract
Techniques are presented to detect 23 isozyme loci in the long-lived perennial plant, ponderosa pine. Meiotically derived megagametophyte from seeds is used to examine directly the segregation of allelic variants. Approximately seven seeds were initially examined for 12 enzymes from each of 47 trees from ten stands throughout the northern Rocky Mountain region. Additional seeds were also examined from selected families to confirm the inheritance of observed electrophoretic variants at 13 polymorphic loci and to estimate linkage relationships. Significant nonrandom segregation was consistently detected for three pairs of loci: ADH-1: AAT-2, ADH-1: PGI-1, and LAP-2: 6PG-1. Preliminary estimates of population parameters reveal a relatively high average heterozygosity (H=0.123). This is partitioned into a high amount of genetic variation within local stands, with only approximately 12% of the total heterozygosity resulting from genic differences between stands.
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References
Allendorf, F. W., Mitchell, N., Ryman, N., and Stahl, G. (1977). Isozyme loci in brown trout (Salmo trutta L.): Detection and interpretation from population data. Hereditas 86179.
Allendorf, F. W., and Utter, F. M. (1978). Population genetics. Fish Physiol. 8407.
Bartels, H. (1971). Genetic control of multiple esterases from needles and macrogametophytes of Picea abies. Planta 99283.
Bergmann, F. (1974). The genetics of some isoenzyme systems in spruce endosperm (Picea abies). Genetika 6353.
Christiansen, F. B., and Frydenberg, O. (1974). Geographical patterns of four polymorphisms of Zoarces viviparus as evidence of selection. Genetics 77765.
Conkle, M. T. (1974). Enzyme polymorphism in forest trees. Proc. 3rd N. Am. Forest Biol. Workshop, p. 95.
Coyne, J. A. (1976). Lack of genic similarity between two sibling species of Drosophila as revealed by varied techniques. Genetics 84593.
Feret, P. P., and Bergmann, F. (1976). Gel electrophoresis of proteins and enzymes. In Miksche, J. (ed.), Modern Methods in Forest Genetics Springer-Verlag, New York, p. 49.
Fildes, R. A., and Harris, H. (1966). Genetically determined variation of adenylate kinase in man. Nature 209261.
Foster, A. S., and Gifford, E. M. (1974). Comparative Morphology of Vascular Plants Freeman, San Francisco.
Fowells, H. A. (1965). Silvics of Forest Trees of the United States Agriculture Handbook No. 271, U.S.D.A. Forest Service, Washington, D.C.
Fowler, D. P., and Morris, R. W. (1977). Genetic diversity in red pine: Evidence for low genic heterozygosity. Can. J. Forest. Res. 7343.
Guries, R. P., and Ledig, T. (1978). Inheritance of some polymorphic isoenzymes in pitch pine (Pinus rigida Mill.). Heredity 4027.
Huang, A. H. C., Liu, K. D. F., and Youle, R. J. (1976). Organelle-specific isozymes of aspartate-alpha-ketoglutarate transaminase in spinach leaves. Plant Physiol. 58110.
Johnson, G. B. (1977). Characterization of electrophoretically cryptic variation in the alpine butterfly Colias meadii. Biochem. Genet. 15665.
Levin, D. A. (1975). Genetic correlates of translocation heterozygosity in plants. Bioscience 25724.
Li, C. C., and Horvitz, D. G. (1953). Some methods of estimating the inbreeding coefficient. Am. J. Hum. Genet. 5107.
Lundkvist, K. (1975). Inheritance of acid phosphotase isozymes in Picea abies. Hereditas 79221.
Lundkvist, K. (1977). Inheritance of esterases in needles and endosperms of Norway spruce (Picea abies K.). Hereditas 8727.
Madsen, J. L., and Blake, G. M. (1977). Ecological genetics of ponderosa pine in the northern Rocky Mountains. Silvae Genet. 261.
Marshall, D. R., Broue, P., and Oram, R. N. (1974). Genotypic control of alcohol dehydrogenase isozymes in narrow-leaf lupins. J. Hered. 65198.
Mitton, J. B., Linhart, Y. B., Hamrick, J. L., and Beckman, J. S. (1977). Observations on the genetic structure and mating systems of ponderosa pine in the Colorado Front Range. Theor. Appl. Genet. 515.
Morris, R. W., and Spieth, P. T. (1978). Sampling strategies for using female gametophytes to estimate heterozygosity in conifers. Theor. Appl. Genet. 51217.
Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. 703321.
Nei, M. (1975). Molecular Population Genetics and Evolution. North-Holland, New York.
Nei, M., and Roychoudhury, A. K. (1974). Sampling variances of heterozygosity and genetic distance. Genetics 76379.
Nevo, E. (1978). Genetic variation in natural populations: patterns and theory. Theor. Pop. Biol. 13121.
Ridgway, G. J., Sherburne, S. W., and Lewis, R. D. (1970). Polymorphism in the esterases of Atlantic herring. Tr. Am. Fish. Soc. 99147.
Rudin, D. (1975). Inheritance of glutamate-oxalate-transaminase (GOT) from needles and endosperms of Pinus sylvestris. Hereditas 80296.
Rudin, D. (1977). Leucine-amino-peptidases (LAP) from needles and macrogametophytes of Pinus sylvestris L.: Inheritance of allozymes. Hereditas 85219.
Simonsen, V., and Wellendorf, H. (1975). Some polymorphic isoenzymes in the seed endosperm of sitka spruce (Picea sitchensis). Forest Tree Improv. 91.
Tigerstedt, P. M. A. (1973). Studies on isozyme variation in marginal and central populations of Picea abies. Hereditas 7547.
Torres, I. (1976). Dissociation-recombination of intergenic sunflower alcohol-dehydrogenase isoenzymes and relative isoenzyme activities. Biochem. Genet. 1487.
Wright, S. (1965). The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19395.
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Funds for this study were made available under the McIntire-Stennis program of the Forest, Range, and Conservation Experiment Station of the School of Forestry, University of Montana.
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O'Malley, D.M., Allendorf, F.W. & Blake, G.M. Inheritance of isozyme variation and heterozygosity in Pinus ponderosa . Biochem Genet 17, 233–250 (1979). https://doi.org/10.1007/BF00498965
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DOI: https://doi.org/10.1007/BF00498965