Summary
Under normal and ischemic conditions backdiffusion of radiolabeled non-esterified fatty acids (NEFA) has been demonstrated. In the fasted normal canine heart the extraction fraction (EF) during interventions with glucose or lactate loading, vasodilation, and metabolic level augmentation was determined, and compared with the control EF. Backdiffusion alterations were deduced from the EF changes. After iv injection of 17-iodo-131 heptadecanoic acid (IHDA), 11 blood samples were drawn from aorta and coronary sinus in a time period of 60 minutes. In the control and vasodilation group the EF slowly decreased from 40 to 10%. In contrast, the EF in the noradrenaline group was constant. During glucose and lactate infusion the EF became negative within 10 min and remained negative. These results suggest that during physiological circumstances backdiffusion is determined by the metabolic level of the heart and its substrate availability.
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References
Fox KA, Abendschein DR, Ambos HD, Sobel BE, Bergmann SR: Effect of metabolized and nonmetabolized fatty acid from canine myocardium. Implications for quantifying myocardial metabolism tomographically. Circ Res 57: 232–243, 1985
Rosamond TL, Abendschein DR, Sobel BE, Bergmann SR, Fox KAA: Metabolic fate of radiolabeled palmitate in ischemic canine myocardium: Implications for positron emission tomography. J Nucl Med 28: 1322–1329, 1987
Folch J, Lees M, Sloane Stanly GH: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509, 1957
Lassers BW, Kaijser L, Carlson LA: Myocardial lipid and carbohydrate metabolism in healthy, fasting men at rest: studies during continuous infusion of 3H palmitate. Eur J Clin Invest 2: 348–358, 1972
Altura BM, Altura BT: Interactions of locally produced humoral substances in regulation of the microcirculation. In: Van Houtte PM and Lensen I. Mechanisms of vasodilation. Karger New York 98–106, 1978
Rose CP, Goreski CA: Constraints on the uptake of labeled palmitate by the heart. Circ Res 41: 534–545, 1977
Bielefeld DR, Vary TC, Neely JR: Inhibition of carnitine palmitoyl-Co-A transferase activity and fatty acid oxidation by lactate and oxfenicine in cardiac muscle. J Mol Cell Cardiol 17: 619–625, 1985
Duwel CMB, Visser FC, Eenige MJ van, Roos JP: Pacing and the noninvasive evaluation of myocardial fatty acid metabolism by means of 17-iodo-123 heptadecanoic acid scintigraphy. Nucl Med 27: 204–208, 1988
Duwel CMB, Visser FC, Eenige MJ van, Roos JP: Results of labeled FFA exercise testing in the normal human myocardium: a new interpretation. Nucl Med 1989 (in press)
Visser FC, Eenige van MJ, Westera G, Den Hollander W, Duwel CMB, Van der Wall EE, Heidendal GAK, Roos JP: Metabolic fate of radioiodinated heptadecanoic acid in the normal canine myocardium. Circ 72: 565–571, 1985
Visser FC, Eenige van MJ, Duwel CMB, Roos JP: Radioiodinated free fatty acids: can we measure myocardial metabolism? Eur J Nucl Med 12: S20–S23, 1986
Katz AM, Messineo FC: Lipid membrane interactions and the pathogenesis of ischemic damage in the myocardium. Circ Res 48: 1–16, 1981
Liedtke AJ, Nellis SH, Neely JR: Effects of excess free fatty acids on mechanical and metabolic function in normal and ischemic myocardium in swine. Circ Res 43: 652–661, 1978
Shug AL, Thomassen JH, Folts JD, Bittar N, Klein MI, Koke JR, Huth PJ: Changes in tissue levels of carnitine and other metabolites during myocardial ischemia and anoxia. Arch Biochem Biophys 187: 25–33, 1978
Van der Vusse GJ, Roemen THM, Prinzen FW, Coumans WA, Reneman RS: Uptake and tissue content of fatty acids in dog myocardium under normoxic and ischemic conditions. Circ Res 50: 538–546, 1982
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© 1989 Kluwer Academic Publishers
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Duwel, C.M.B., Visser, F.C., van Eenige, M.J., Roos, J.P. (1989). Variables of myocardial backdiffusion, determined with 17-IODO-131 heptadecanoic acid in the normal dog heart. In: Van Der Vusse, G.J. (eds) Lipid Metabolism in Normoxic and Ischemic Heart. Developments in Molecular and Cellular Biochemistry, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1611-4_28
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DOI: https://doi.org/10.1007/978-1-4613-1611-4_28
Publisher Name: Springer, Boston, MA
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