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Methods of Reconstitution of Ion Channels

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Molecular Biology of Membrane Transport Disorders

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Abstract

The advent of single-channel technology has expanded the physiologist’s ability to understand gross cellular and organellar function at the molecular level. At the same time that we are increasingly employing these microscopic techniques to characterize the molecular aspects of ion channels, we are better able to define their macroscopic behavior. Although the goal of many of these studies is to identify a specific ion channel with a specific cellular or organellar function, there are numerous problems and limitations in confirming this identity. Frequently, for example, there may be several distinct channel types present in the same membrane. The identification of a function with a specific ion channel thus depends on the characterization of the activity at the cellular and at the molecular, single-channel level. Several broad approaches are routinely applied to study the molecular aspects of specific ion channels. Patch clamp methodology identifies the channels in native membranes and provides the biophysical fingerprint as well as key regulatory features.

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Authors and Affiliations

  1. Department of Integrative Biology, The University of Texas-Houston Medical School, 77225, Houston, Texas, USA

    William P. Dubinsky & Otilia Mayorga-Wark

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  1. William P. Dubinsky
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Editors and Affiliations

  1. University of Texas Medical School at Houston, Houston, Texas, USA

    Stanley G. Schultz

  2. University of Arkansas College of Medicine, Little Rock, Arkansas, USA

    Thomas E. Andreoli

  3. Case Western Reserve University, Cleveland, Ohio, USA

    Arthur M. Brown

  4. The Johns Hopkins University, Baltimore, Maryland, USA

    Douglas M. Fambrough

  5. Yale University School of Medicine, New Haven, Connecticut, USA

    Joseph F. Hoffman

  6. University of Iowa College of Medicine, Iowa City, Iowa, USA

    Michael J. Welsh

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© 1996 Plenum Press, New York

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Dubinsky, W.P., Mayorga-Wark, O. (1996). Methods of Reconstitution of Ion Channels. In: Schultz, S.G., Andreoli, T.E., Brown, A.M., Fambrough, D.M., Hoffman, J.F., Welsh, M.J. (eds) Molecular Biology of Membrane Transport Disorders. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1143-0_4

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  • DOI: https://doi.org/10.1007/978-1-4613-1143-0_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8446-8

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