Abstract
The analysis of lipids is dominated by chromatographic methods such as gas chromatography (GC) and high-pressure liquid chromatography (HPLC) and some other physical methods such as thermo analysis. Nuclear magnetic resonance (NMR) spectroscopy covers only a small part of lipid analysis. In most cases it is used for qualitative analysis such as structure elucidation of lipids. Unlike the other methods NMR spectroscopy is coupled with computer technology, and its development is as fast as that of computer software and hardware. In 1991 the Nobel Prize for chemistry was given to Professor Ernst for the development of Fourier Transform NMR spectroscopy (FT-NMR). The capabilities of these methods even for the elucidation of the three-dimensional structures of small molecules up to macromolecules are impressive.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aitzetmüller, K., Diehl, B. W. K., Ockels, W. and Herling, H. (1994) Investigation of seed oils containing Δ-5 and Δ-6 fatty acids using 13C-NMR-spectroscopy. Joint Congress of 2nd EUROLIPID and 50th DGF Conference, Münster, Germany, September; copy available from author.
Aitzetmüller, K. and Spectral Service, in production; copy available from author.
Aursand, M. and Grasdalen, H. (1992) Interpretation of the 13C-NMR spectra of omega-3 fatty acids and lipid extracted from the white muscle of Atlantic salmon (Salmo salar). Chemistry and Physics of Lipids, 62, 239–51.
Aursand, M., Jørgensen, L. and Grasdalen, H. (1995) Positional distribution of ω-3 fatty acids in marine lipid triacylglycerols by high-resolution 13C-nuclear Magnetic resonance spectroscopy. J. Am. Oil Chem. Soc., 72 (3), 293–7.
Danz, B. (1993) Storage Stability of Liposomes. Diploma Thesis, Fachhochschule Magdeburg, Germany.
De Kock, J. (1993) The European Analytical Subgroup of I.L.P.S. — a joint effort to clarify lecithin and phospholipid analysis. Fat Sci. Technol., 95 (9), 352–5.
De Kock, J. (1995) The European Analytical Subgroup of I.L.P.S., in Characterization, Metabolism and Novel Biological Applications (eds G. Cevc and F. Paltauf), AOCS Press, Champaign, pp. 22–28.
Dennis, E. A. and Plückthun, A. (1984) Phosphorus-31 NMR of phospholipids in micelles, in Phosphorus-31 NMR, Principles and Applications, Academic Press, New York, pp. 423–46.
Diehl, B. W. K. (1993) 1H-, 13C- and 31P-nuclear magnetic resonance spectroscopy of lipids. AOCS Meeting 1993, Anaheim, Inform 4, 513; copy available from author.
Diehl, B. W. K. and Ockels, W. (1995a) Quantitative analysis of emulsifiers by multinuclear high resolution NMR. 21st World Congress and Exhibition of the International Society for Fat Research, The Hague, Netherlands, October 1995; copy available from author.
Diehl, B. W. K. and Ockels, W. (1995b) Fatty acid distribution by 13C-NMR-spectroscopy. Fat Scientific Technology, 97 (3), 115–18.
Diehl, B. W. K. and Ockels, W. (1995c) Quantitative analysis of phospholipids, in Proceeding of the 6th International Colloquium Phospholipids. Characterization, Metabolism and Novel Biological Applications (eds G. Cevc and F. Paltauf), AOCS Press, Champaign, pp. 29–32.
Diehl, B. W. K. and Stein, J. (1994) Calibration standards for lecithin analysis using HPLC with light scattering detector. 84th AOCS Annual Meeting and Exposition, Atlanta, GA, May 1994; copy available from author.
Diehl, B. W. K., Ockels, W. and Woydt, D. (1994) Kinetics in lecithin degradation. 84th AOCS Annual Meeting and Exposition, Atlanta, GA, May; copy available from author.
Diehl, B. W. K., Herling, H., Heinz, E. and Riedl, I. (1995) 13C-NMR analysis of the positional distribution of fatty acids in plant glycolipids. Chemistry and Physics of Lipids, 77, 147–53.
Diehl, B. W. K., Ockels, W., Herling, H. and Unger, R. (1996a) Das DHA Ei, der Weg der ω-3 Fettsäuren, Multinukleare NMR-Untersuchungen, 51. Jahrestagung der Deutschen Gesellschaft für Fettwissenschaft, Bremen, October; copy available from author.
Diehl, B. W. K., Ockels, W., Herling, H. et al. (1996b) Quantitative determination of egg-lecithin in a liposome-preparation, validation of the 31P-NMR-method. 7th International Congress on Phospholipids, Brussels, September; copy available from author.
Genn, B. (1992) Identification of Phospholipids in Bovine Lung Surfactant. Diploma Thesis. Fachhochschule Aachen, Abteilung Jülich, Germany.
Glonek, T. and Mercant, T. E. (1996) 31P-nuclear magnetic resonance profiling of phospholipids, in Advances in Lipid Methodology — Three (ed. W. W. Christie), The Oily Press, Dundee, pp. 37–75.
Gunstone, F. D. (1990) 13C-NMR spectra of oils containing γ-linolenic acid. Chemistry and Physics of Lipids, 56, 201–7.
Gunstone, F. D. (1991) High resolution NMR studies of fish oils. Chemistry and Physics of Lipids, 59, 83–9.
Gunstone, F. D. (1993a) High resolution 13C-NMR-spectroscopy of lipids, in Advances in Lipid Methodology — Two (ed. W. W. Christie), The Oily Press, Dundee, pp. 1–68.
Gunstone, F. D. (1993b) The 13C-NMR spectra of six oils containing petroselinic acid and of aquilegia oil and meadowfoam oil which contain Δ5 acids. Chemistry and Physics of Lipids, 58, 159–67.
Herling, H. (1995) Fatty Acid Distribution in Lipids. Diploma Thesis, FH Aachen, Abt. Jülich, Germany; copy available from author.
Hossein Nouri-Sorkhabi, M., Wright, L. C, Sullivan, D. R. and Kuchel, P. W. (1996) Quantitative 31P nuclear magnetic resonance analysis of erythrocyte membranes using detergent. Lipids, 31, 765–70.
Mallet, J. F., Gaydou, E. M. and Archavlis, A. (1990) Determination of petroselinic acid in umbelliflorae seed oils by combined GC and 13C-NMR spectroscopy analysis. J. Am. Oil Chem. Soc., 67 (10), 607–10.
Manz, I. and Schneider, K. (1995) Bestimmung der Jodzahl in epoxidiertem Sojabohnenöl mit automatisierter 1H-NMR Spektroskopie. GIT Fachzeitschrift für Laboratorien, 3, 197–9.
Meneses, P. and Glonek, T. (1988) High resolution 31P-NMR-spectroscopy of extracted phospholipids. Journal of Lipid Research, 29, 679–89.
Ng, S. (1985) Analysis of positional distribution of fatty acids in palm oil by 13C-NMR spectroscopy. Lipids, 20, 778–82.
Ng, S. and Koh, H. F. (1988) Detection of cis-vaccenic acid in palm oil by 13C-NMR spectroscopy. Lipids, 23, 140–3.
Pearce, J. M. and Komoroski, R. A. (1993) Magnetic Resonance in Medicine, 29, 724–31.
Pfeffer, P. E., Luddy, F. E. and Unruh, J. (1977) 13C-NMR: a rapid, nondestructive method for determining the cis, trans composition of catalytically treated unsaturated lipid mixtures. JOACS, 54 (9), 380–6.
Sacchi, P., Addeo, F., Giudicianni, I. and Paolillo, L. (1990) Applicazione della spettroscopia 13C-NMR alla determinazione di mono-digliceridi ed acidi grassi liberi nell’ olio di oliva di pressione e nei rettificati. La Rivista Italiana délie sostanze grasse, LXVII, 245–52.
Shoolery, J. N. (1976) Some quantitative applications of 13C-NMR spectroscopy. Progress in Nuclear Resonance Spectroscopy, 11(2), 79–93.
Sotirhos, N., Herslöf, B. and Kenne, L. (1986) Quantitative analysis of phospholipids by 31P-NMR. Journal of Lipid Research, 27, 386–92.
Wollenberg, K. F. (1990) Quantitative high resolution 13C nuclear magnetic resonance of the olefinic and carbonyl carbons of edible vegetable oils. JAOCS, 67, (8), 487–94.
Woydt, D. (1994) Kinetics in Lecithin Degradation. Diploma Thesis. Universität Osnabrück, Germany.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Chapman & Hall
About this chapter
Cite this chapter
Diehl, B.W.K. (1998). Multinuclear high-resolution nuclear magnetic resonance spectroscopy. In: Hamilton, R.J. (eds) Lipid Analysis in Oils and Fats. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1131-7_4
Download citation
DOI: https://doi.org/10.1007/978-1-4613-1131-7_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-8432-1
Online ISBN: 978-1-4613-1131-7
eBook Packages: Springer Book Archive