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Regulation of Cytochrome P-450 Biosynthesis in Alkane Assimilating Yeast

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Metabolism and Enzymology of Nucleic Acids

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Summary

In the yeast Candida maltosa both the substrate (n-alkane) and oxygen mediated induction of the alkane hydroxylating P-450 were shown to be regulated transcriptionally. That was concluded from good correlations of P-450 protein content (spectral determination of intact holo-enzyme and radioimmunological quantification of apo-enzyme) and relative amount of P-450 mRNA (in vitro translation with subsequent immunoprecipitation).

P-450 was taken as a model to investigate the biosynthesis of membrane proteins in homologous and heterologous cell-free translation/translocation systems. P-450 as well as alpha-factor could be translocated into mammalian and yeast membranes.

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Abbreviations

P-450:

cytochrome P-450

SRP:

signal recognition partical

RIA:

radioimmunoassay

C. maltosa:

Candida maltose

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Authors and Affiliations

  1. Academy of Sciences of the GDR, Central Institute of Molecular Biology, DDR 1115, Robert-Rossle Str. 10, Berlin-Buch, Germany

    B. Wiedmann, M. Wiedmann, W. H. Schunck, S. Mauersberger, E. Kargel & H. G. Müller

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  1. B. Wiedmann
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  2. M. Wiedmann
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  3. W. H. Schunck
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  4. S. Mauersberger
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  5. E. Kargel
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  6. H. G. Müller
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Editor information

Editors and Affiliations

  1. Slovak Academy of Sciences, Bratislava, Czechoslovakia

    Ján Zelinka  & Jozef Balan  & 

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© 1988 Plenum Press, New York

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Wiedmann, B., Wiedmann, M., Schunck, W.H., Mauersberger, S., Kargel, E., Müller, H.G. (1988). Regulation of Cytochrome P-450 Biosynthesis in Alkane Assimilating Yeast. In: Zelinka, J., Balan, J. (eds) Metabolism and Enzymology of Nucleic Acids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0749-5_40

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  • DOI: https://doi.org/10.1007/978-1-4613-0749-5_40

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8063-7

  • Online ISBN: 978-1-4613-0749-5

  • eBook Packages: Springer Book Archive

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