Abstract
Transition of n-hexadecane utilizing cultures of Candida maltosa to oxygen-limited growth caused an up to 6-fold increase of the cellular cytochrome P-450 content. Enhanced cytochrome P-450 formation required protein de novo synthesis and was not due to a change of the apo/holo-enzyme ratio as demonstrated by cycloheximide inhibition and immunological quantitation. The effect of low oxygen concentration (pO2=3–5%) was simulated by selective inhibition of alkane hydroxylation with carbon monoxide (at a pO2 of 70–75%). Enhanced cytochrome P-450 formation occurred even when a constant growth rate was maintained through utilization of a second non-repressive growth substrate. However, the presence of n-alkanes was an essential precondition. It was concluded, that the cytochrome P-450 formation was mainly regulated by the intracellular inducer concentration which depends on the relative rates of alkane transport into the cell and the actual alkane hydroxylating activity of the enzyme system.
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Abbreviations
- cyt:
-
cytochrome
References
Aoyama Y, Yoshida Y, Sato R (1984) Yeast cytochrome P-450 catalyzing lanosterol 14α-demethylation. II. Lanosterol metabolism by purified P-450DM and by intact microsomes. J Biol Chem 259:1661–1666
Gmünder FK, Käppeli O, Fiechter A (1981) Chemostat studies on the hexadecane assimilation by the yeast Candida tropicalis. II. Regulation of cytochromes and enzymes. Eur J Appl Microbiol Biotechnol 12:135–142
Ishidate K, Kawaguchi K, Tagawa K (1969) Change in cytochrome P-450 content accompanying aerobic formation of mitochondria in yeast. J Biochem (Tokyo) 65:385–392
Kärgel E, Schmidt HE, Schunck W-H, Riege P, Mauersberger S, Müller H-G (1984) A solid phase radio-immuno-assay for yeast cytochrome P-450. Anal Lett 17(B18):2011–2024
Kärgel E, Schunck W-H, Riege P, Honeck E, Claus R, Kleber H-P, Müller H-G (1985) A comparative immunological investigation of the alkane hydroxylating cytochrome P-450 from the yeast Candida maltosa. Biochem Biophys Res Commun 128:1261–1267
Laurila H, Käppeli O, Fiechter A (1984) The cytochrome P-450 containing monooxygenase of Trichosporon cutaneum: Occurence and properties. Arch Microbiol 140:257–259
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Mauersberger S, Matyashova RN, Müller H-G, Losinov AB (1980) Influence of the growth substrate and the oxygen concentration in the medium on the cytochrome P-450 content in Candida guilliermondii. Eur J Appl Microbiol Biotechnol 9:285–294
Mauersberger S, Schunck W-H, Müller H-G (1984) The induction of cytochrome P-450 in Lodderomyces elongisporus. Z Allg Mikrobiol 21:313–321
Mauersberger S, Schunck W-H, Müller H-G (1984) The induction of cytochrome P-450 in the alkane utilizing yeast Lodderomyces elongisporus: Alterations in the microsomal membrane fraction. Appl Microbiol Biotechnol 19:29–35
Omura T, Sato R (1964) The carbon monoxide-binding pigment of liver microsomes. II. Solubilization, purification and properties. J Biol Chem 239:2379–2385
Rogers PJ, Stewart PR (1973) Respiratory development in Saccharomyces cerevisiae grown at controlled oxygen tension. J Bacteriol 155:88–93
Sanglard D, Käppeli O, Fiechter A (1984) Metabolic conditions determining the composition and catalytic activity of cytochrome P-450 monooxygenases in Candida tropicalis. J Bacteriol 157:297–302
Schunck WH, Mauersberger S, Huth J, Riege P, Müller HG (1987) Function and regulation of cytochrome P-450 in alkane-assimilating yeast I. Selective inhibition with carbon monoxide in growing cells. Arch Microbiol 147:240–244
Sharyshev AA, Matyashova RN, Komarova GN (1983) Cytochrome P-450 assay in Candida and Saccharomyces cells under various growth conditions. FEMS Symp Environ Reg Microbiol Metabolism, Pushchino 1983, Abstract p 33
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Schunck, W.H., Mauersberger, S., Kärgel, E. et al. Function and regulation of cytochrome P-450 in alkane-assimilating yeast. Arch. Microbiol. 147, 245–248 (1987). https://doi.org/10.1007/BF00463483
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DOI: https://doi.org/10.1007/BF00463483