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Genetic Engineering of Antibiotic-Producing Microorganisms

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The Search for Bioactive Compounds from Microorganisms

Part of the book series: Brock/Springer Series in Contemporary Bioscience ((BROCK/SPRINGER))

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Abstract

Many antibiotics are produced by strains of the genus Streptomyces. Although they are classified as gram-positive organisms, the streptomycetes exhibit a more complicated life cycle than do Bacillus and also show greater differentiation. The genomic size is about 6000 kbp, 1.6 times larger than that of E. coli (Genthner et al., 1985). Genetic manipulation in Streptomyces has been established by D.A. Hopwood and his coworkers (Hopwood et al., 1985a). So far, treatment with mutagens and the screening of resulting clones has been repeated and adopted as a procedure for the breeding of antibiotic-producing microorganisms and has produced good results.

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References

  • Bailey, C. R., Butler, M. J., Normansell, I. D., Rowlands, R. T. and Winstanley, D. J. 1984: Cloning a Streptomyces clavuligerus genetic locus involved in clavulanic acid biosynthesis. Biotechnology 2:808–811.

    Article  CAS  Google Scholar 

  • Chater, K. F. and Bruton, C. J. 1983: Mutational cloning in Streptomyces and the isolation of antibiotic production genes. Gene 26:67–78.

    Article  PubMed  CAS  Google Scholar 

  • Chater, K. F. and Bruton, C. J. 1985: Resistance, regulatory and production genes for the antibiotic methylenomycin are clustered. EMBO Journal. 4:1893–1892.

    PubMed  CAS  Google Scholar 

  • Distler, J., Braun, C., Ebert, A. and Piepersberg, W. 1987: Gene cluster for streptomycin biosynthesis in Streptomyces griseus: Analysis of a central region including the major resistance gene. Molecular & General Genetics 208:204–210.

    Article  CAS  Google Scholar 

  • Feitelson, J. S. and Hopwood, D. A. 1983: Cloning of a Streptomyces gene for a O-methyltransferase involved in antibiotic biosynthesis. Molecular & General Genetics 190:394–398.

    Article  CAS  Google Scholar 

  • Feitelson, J. S., Malpartida, F. and Hopwood, D. A. 1985: Genetic and biochemical characterization of the red gene cluster of Streptomyces coelicolor A3(2). Journal of General Microbiology 131:2431–2441.

    PubMed  CAS  Google Scholar 

  • Fishman, S. E., Cox, K., Larson, J. L., Reynolds, P. A., Seno, E. T., Yeh, W.-K., Van Frank, R. and Hershberger, C. L. 1987: Cloning genes for the biosynthesis of a macrolide antibiotic. Proceedings of the National Academy Science of the United States of America 84:8248–8252.

    Article  CAS  Google Scholar 

  • Genthner, F. J., Hook, L. A. and Strohl, W. R. 1985: Determination of the molecular mass of bacterial genomic DNA and plasmid copy number by high-pressure liquid chromatography. Applied and Enviromental Microbiology 50:1007–1013.

    CAS  Google Scholar 

  • Gil, J. A., Hopwood, D. A. 1983: Cloning and expression of a p-aminobenzoic acid synthetase gene of the candicidin-producing Streptomyces griseus. Gene 25:119–132.

    Article  PubMed  CAS  Google Scholar 

  • Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, J., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. and Schrempf, H. 1985a: Genetic manipulations of Streptomyces: A LABORATORY MANUAL. John Innes Foundation, Norwich, England.

    Google Scholar 

  • Hopwood, D. A., Malpartida, F., Kieser, H. M., Ikeda, H., Duncan, J., Fujii, I., Rudd, B. A. M., Floss, H. G., Ōmura, S. 1985b: Production of ‘hybrid’ antibiotics by genetic engineering. Nature (London) 314:642–644.

    Article  CAS  Google Scholar 

  • Horinouchi, S., Hara, O. and Beppu, T. 1983: Cloning of a pleiotropic gene that positively controls biosynthesis of A-factor, actinorhodin, and prodigiosin in Streptomyces coelicolor A3(2) and Streptomyces lividans. Journal of Bacteriology 155:1238–1248.

    PubMed  CAS  Google Scholar 

  • Horinouchi, S. and Beppu, T. 1984a: Production in large quantities of actinorhodin and undecylprodigiosin induced by afsB in Streptomyces lividans. Agricultural and Biological Chemistry 48:2131–2133.

    Article  CAS  Google Scholar 

  • Horinouchi, S., Kumada, Y. and Beppu, T. 1984b: Unstable genetic determinant of A-factor biosynthesis in streptomycin-producing organisms: Cloning and characterization. Journal of Bacteriology 158:481–487.

    CAS  Google Scholar 

  • Jones, G. H. and Hopwood, D. A. 1984: Molecular cloning and expression of the phenoxazinone synthase gene from Streptomyces antibioticus. Journal of Biological Chemistry 259:14151–14157.

    PubMed  CAS  Google Scholar 

  • Khokhlov, A. S., Tovarova, I.I., Borisova, L. N., Pliner, S. A., Shevchenko, L. A., Kornitskaya, E. Y., Ivkina, N. S. and Rapoport, I. A. 1967: A-factor responsible for the biosynthesis of streptomycin by a mutant strain of Actinomyces streptomycini. Akademii Nauk Uzbekskoi SSR. 177:232–235.

    CAS  Google Scholar 

  • Malpartida, F. and Hopwood, D. A. 1984: Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host. Nature (London) 309:462–464.

    Article  CAS  Google Scholar 

  • Malpartida, F. and Hopwood, D. A. 1986: Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2). Molecular & General Genetics 205:66–73.

    Article  CAS  Google Scholar 

  • Matsushima, P. and Baltz, R. H. 1985: Efficient plasmid transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts. Juornal of Bacteriology 163:180–185.

    CAS  Google Scholar 

  • McAlpaine, J. B., Tuan, J. S., Brown, D. P., Grebner, K. D., Whittern, D. N., Buko, A. and Katz, L. 1987: New antibiotics from genetically engineered actinomycetes. I. 2-norerythromycin, Isolation and structural determination. Journal of Antibiotics (Tokyo) 40:1115–1122.

    Google Scholar 

  • McCann, P. A., Pogell, B. M. 1979: Pamamycin: A new antibiotic and stimulator of aerial mycelia formation. Journal of Antibiotics (Tokyo) 32:673–678.

    CAS  Google Scholar 

  • Murakami, T., Anzai, H., Imai, S., Satoh, A., Nagaoka, K. and Thompson, C. J. 1986: The bialaphos biosynthetic genes of Streptomyces hygroscopicus: Molecular cloning and characterization of the gene cluster. Molecular & General Genetics 205:42–50.

    Article  CAS  Google Scholar 

  • Ogawa, H., Imai, S., Satoh, A. and Kojima, M. 1983: An improved method for the preparation of Streptomyces and Microspora protoplasts. Journal of Antibiotics (Tokyo) 36:184–186.

    CAS  Google Scholar 

  • Ohnuki, T., Imanaka, T. and Aiba, S. 1985: Self-cloning in Streptomyces griseus of an str gene cluster for streptomycin biosynthesis and streptomycin resistance. Journal of Bacteriology 164:85–94.

    PubMed  CAS  Google Scholar 

  • Ōmura, S., Ikeda, H., Malpartida, F., Kieser, H. M. and Hopwood, D. A. 1986: Production of a new hybrid antibiotics mederrhodins A and B by genetically engineered strain. Antimicrobial Agents and Chemotherapy 29:13–19.

    PubMed  Google Scholar 

  • Rodicio, M. R. and Chater, K. F. 1982: Small DNA-free liposomes stimulate transfection of Streptomyces protoplasts. Journal of Bacteriology 151:1078–1085.

    PubMed  CAS  Google Scholar 

  • Rudd, B. A. M. and Hopwood, D. A. 1979: Genetics of actinorhodin biosynthesis by Streptomyces coelicolor A3(2). Journal General Microbiology 114:35–43.

    CAS  Google Scholar 

  • Samson, S. M., Belagaje, R., Blankenship, D. T, Chapman, J. L., Perry, D., Skatrud, P. L., Van Frank, R. M., Abraham, E. P., Baldwin, J. E., Queener, S. W. and Ingolia, T. D. 1985: Isolation sequence determination and expression in Esherichia coli of the isopenicillin N synthetase gene from Cephalosporium acremonium. Nature (London) 318:191–194.

    Article  CAS  Google Scholar 

  • Scribner, H. E., III, Tang, T. and Bradley, S. G. 1973: Production of a sporulation pigment by Streptomyces venezuelae. Applied Microbiology 25:873–879.

    PubMed  CAS  Google Scholar 

  • Stanzak, P., Matsushima, P., Baltz, R. H. and Rao, R. N. 1986: Cloning and expression in Streptomyces lividans of clustered erythromycin biosynthesis genes from Streptomyces erythreus. Biotechnology 4:229–232.

    Article  CAS  Google Scholar 

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Authors
  1. Haruo Ikeda
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Editors and Affiliations

  1. Research Center for Biological Function, The Kitasato Institute, and School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, 108, Japan

    Satoshi Ōmura Ph.D.

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© 1992 Spring-Verlag New York, Inc.

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Ikeda, H. (1992). Genetic Engineering of Antibiotic-Producing Microorganisms. In: Ōmura, S. (eds) The Search for Bioactive Compounds from Microorganisms. Brock/Springer Series in Contemporary Bioscience. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4412-7_17

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  • DOI: https://doi.org/10.1007/978-1-4612-4412-7_17

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-8761-2

  • Online ISBN: 978-1-4612-4412-7

  • eBook Packages: Springer Book Archive

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