Abstract
The maize pollen grain represents the beginning of a short-lived gametophytic phase during which the two sperm are delivered to the embryo sac prior to fertilization. Although this stage of the life cycle normally consists of only a few cell divisions, under certain experimental conditions, gametophytes can be induced to undergo an altered development, leading to the production of haploid embryolike structures and/or callus, without an intervening fertilization. This remarkable process, referred to as androgenesis, is the biological basis for the in vitro techniques known as anther and microspore culture. This chapter describes some protocols associated with producing doubled haploid maize from cultured anthers and microspores.
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References
Genovesi AD (1990) Maize (Zea mays L.): in vitro production of haploids. In Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 12, Springer-Verlag, Berlin, pp 176–203
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© 1994 Springer-Verlag New York, Inc.
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Petolino, J.F., Genovesi, A.D. (1994). Anther and Microspore Culture. In: Freeling, M., Walbot, V. (eds) The Maize Handbook. Springer Lab Manuals. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2694-9_126
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DOI: https://doi.org/10.1007/978-1-4612-2694-9_126
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-94735-8
Online ISBN: 978-1-4612-2694-9
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