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Characterization and Utilization of Candida rugosa Lipase Immobilized on Controlled Pore Silica

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Twentieth Symposium on Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

Abstract

Candida rugosa lipase was immobilized by covalent binding on controlled pore silica (CPS) using glutaraldehyde as cross-linking agent under aqueous and nonaqueous conditions. The immobilized C. rugosa was more active when the coupling procedure was performed in the presence of a nonpolar solvent, hexane. Similar optima pH (7.5–8.0) was found for both free and immobilized lipase. The optimum temperature for the immobilized lipase was about 10°C higher than that for the free lipase. The thermal stability of the CPS lipase was also greater than the original lipase preparation. Studies on the operational stability of CPS lipase revealed good potential for recycling under aqueous (olive-oil hydrolysis) and nonaqueous (butyl butyrate synthesis) conditions.

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Chemical Engineering of Lorena, PO Box 116, 12600-000, Lorena-SP, Brazil

    Cleide M. F. Soares & Heizir F. De Castro

  2. Department of Chemical Engineering, Maringá State University, Maringá-PR, Brazil

    Flávio F. De Moraes & Gisella M. Zanin

Authors
  1. Cleide M. F. Soares
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  2. Heizir F. De Castro
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  3. Flávio F. De Moraes
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  4. Gisella M. Zanin
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Corresponding author

Correspondence to Heizir F. De Castro .

Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Brian H. Davison

  2. National Renewable Energy Laboratory, USA

    Mark Finkelstein

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© 1999 Springer Science+Business Media New York

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Soares, C.M.F., De Castro, H.F., De Moraes, F.F., Zanin, G.M. (1999). Characterization and Utilization of Candida rugosa Lipase Immobilized on Controlled Pore Silica. In: Davison, B.H., Finkelstein, M. (eds) Twentieth Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1604-9_68

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  • DOI: https://doi.org/10.1007/978-1-4612-1604-9_68

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-7214-4

  • Online ISBN: 978-1-4612-1604-9

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