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Immobilization of lipase on carboxylic acid-modified silica nanoparticles for olive oil glycerolysis

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Abstract

In this study, the silica nanoparticles surface was modified by carboxylic acid with different number of alkyl chain and two different lipases were immobilized on it to increase the glycerolysis activity of the immobilized lipases in organic solvent system. Silica nanoparticles of about 15 nm diameter were grafted with various carboxylic acid modifiers from valeric, caprylic, capric, lauric, stearic and oleic acids. Lipases from Candida antarctica and Candida rugosa were immobilized onto the carboxylic acid, modified silica nanoparticles and used in the mono and diglycerides production through glycerolysis with or without organic solvents. When lipases immobilized on stearic acid-modified silica nanoparticles, both lipases gave higher activity compared to their corresponding free lipases. Immobilized C. rugosa were stable and reused for 11 cycles without loss in activity. The kinetic parameters, K m and V max of free and immobilized lipases were found using Lineweaver–Burk model. Results indicated that the immobilized lipase had a lower K m and V max when compared with the free lipase.

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Acknowledgements

The authors wish to thank Dr. Sanjay M. Mahajani, Chemical Engineering Department, IIT Bombay for using HPLC of this research. The authors are grateful to the Sophisticated Analytical Instrument Facility (SAIF), IIT Bombay, for providing the research facility for characterizations of samples.

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Authors and Affiliations

  1. Department of Chemical Engineering, Sardar Vallabhbhai National Institute of Technology, Surat, Gujarat, 395007, India

    Abhishek Kumar Singh & Mausumi Mukhopadhyay

  2. University Institute of Biotechnology, Chandigarh University, Mohali, Punjab, 140413, India

    Abhishek Kumar Singh

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  1. Abhishek Kumar Singh
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  2. Mausumi Mukhopadhyay
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Correspondence to Mausumi Mukhopadhyay.

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Singh, A.K., Mukhopadhyay, M. Immobilization of lipase on carboxylic acid-modified silica nanoparticles for olive oil glycerolysis. Bioprocess Biosyst Eng 41, 115–127 (2018). https://doi.org/10.1007/s00449-017-1852-5

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  • DOI: https://doi.org/10.1007/s00449-017-1852-5

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