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Synthesis of Monocaprin Catalyzed by Lipase

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Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

Abstract

The production of monoglyceride emulsifiers commonly employed in the food, cosmetic, and pharmaceutical industries can be catalyzed by lipases, biocatalysts that are becoming increasingly attractive in the enzyme market. The aim of this study was to produce monocaprin utilizing a commercial immobilized lipase (Lipozyme IM 20) through the direct esterification of capric acid and glycerol. Experiments were performed for 6 h in an open reactor and the products were analyzed by gas chromatography. The parameters investigated were the amount of enzyme, temperature, and molar ratio between the reagents (capric acid/glycerol). The experimental runs followed an experimental design generated using Statistica® software. The results showed that all the parameters were significant and that monocaprin production was enhanced at the lower ranges of the tested variables. The best conditions established were 55°C, 3% (w/w) enzyme concentration, and molar ratio of 1. The final product, obtained after 6 h of reaction, was 61.3% monocaprin, 19.9% dicaprin, and 18.8% capric acid. This composition satisfies the directives of the World Health Organization food emulsifiers, which requires that these mixtures have at least 70% mono- plus diglyceride, and a minimum of 30% monoacylglycerol.

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Authors and Affiliations

  1. DBq/Instituto de Química, Universidade Federal do Rio de Janeiro, Prédio do Centro de Tecnologia, Bloco A, Lab. 549-2, Ilha do Fundão, 21949-900, Rio de Janeiro/RJ, Brasil

    Marco A. M. Da Silva & Denise M. G. Freire

  2. Instituto de Química, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil

    Vitória C. Medeiros & Marta A. P. Langone

Authors
  1. Marco A. M. Da Silva
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  2. Vitória C. Medeiros
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  3. Marta A. P. Langone
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  4. Denise M. G. Freire
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Corresponding author

Correspondence to Denise M. G. Freire .

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Editors and Affiliations

  1. Oak Ridge National Laboratory, Oak Ridge, TN, USA

    Brian H. Davison (Conference Chair) & James W. Lee (Conference Chair) & 

  2. National Renewable Energy Laboratory, Golden, CO, USA

    Mark Finkelstein (Conference Co-Chair) & James D. McMillan (Conference Co-Chair) & 

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Da Silva, M.A.M., Medeiros, V.C., Langone, M.A.P., Freire, D.M.G. (2003). Synthesis of Monocaprin Catalyzed by Lipase. In: Davison, B.H., Lee, J.W., Finkelstein, M., McMillan, J.D. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0057-4_63

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  • DOI: https://doi.org/10.1007/978-1-4612-0057-4_63

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6592-4

  • Online ISBN: 978-1-4612-0057-4

  • eBook Packages: Springer Book Archive

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