Halothane and Sevoflurane Exert Different Degrees of Inhibition on Carotid Body Glomus Cell Intracellular Ca2+ Response to Hypoxia

  • Jaideep J. Pandit
  • Keith J Buckler
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 669)


The purpose of this study was to ascertain if effects of halothane and sevoflurane (0.18–1.45 MAC) on the magnitude of the rise in intracellular calcium ([Ca2+]i with ∼90s hypoxia (measured using indo-1 dye) in rat pup carotid body type I glomus cells. paralleled their known effects on the human hypoxic ventilatory response, where halothane is more depressive. We also assessed these agents’ effect on [Ca2+]i response to 100 mM K+. Halothane depressed the [Ca2+]i transient in hypoxia more than sevoflurane (p = 0.036). Both agents also depressed the [Ca2+]i response to K+ – halothane more than sevoflurane (p = 0.004). These actions reflect their known influence on human hypoxic ventilatory response, consistent with the notion that the cellular process underlies the whole-body effect. The responses to K+, which depolarises the cell membrane, indicates that in addition to a putative effect on K+ channels, voltage-activated Ca2+ channels may also be involved in the anaesthetic effect.


Contrasting Effect Carotid Body Volatile Anaesthetic Minimum Alveolar Concentration Hypoxic Response 
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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Nuffield Department of AnaestheticsJohn Radcliffe HospitalOxfordUK
  2. 2.Department of Physiology, Anatomy and GeneticsUniversity of OxfordOxfordUK

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