Effect of JM-1232(-), a New Sedative on Central Respiratory Activity in Newborn Rats

  • Junya Kuribayashi
  • Shun-ichi Kuwana
  • Yuki Hosokawa
  • Eiki Hatori
  • Junzo Takeda
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 669)


JM-1232(-), a newly manufactured isoindole derivative, shows sedative effect at a lower concentration compared with propofol. In the present study, we analyzed the response of the central respiratory activity to JM-1232(-). The brainstem-spinal cord of a newborn rat was isolated and was continuously superfused with oxygenated artificial cerebrospinal fluid (ACSF). Rhythmic inspiratory burst activity was recorded from C4 spinal ventral root using a glass suction electrode. We measured C4 burst rate and amplitude of integrated C4 activity. After obtaining a control recording, the preparation was superfused with ACSF containing JM-1232(-) at 10, 100 or 500 μM for 10 min. The application of both 10 and 100 μM JM-1232(-) did not decrease C4 burst rate significantly. However, 500 μM JM-1232(-) reduced C4 burst rate. On the contrary, C4 burst amplitude was not affected by the application of JM-1232(-) for 10 min at any concentrations. In conclusion, JM-1232(-) at a low concentration (but presumably higher than hypnotic dose), did not depress the central respiratory activity, whereas at a high concentration depression was seen.


Respiratory Depression Ventral Root Artificial Cerebrospinal Fluid Anesthetic Dose Hypnotic Dose 
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This work was Supported by Japan Society for the Promotion of Science KAKENHI (18791106 to Dr. Kuribayashi and 19603015 to Dr. Kuwana), Tokyo, Japan


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Junya Kuribayashi
    • 1
    • 2
    • 3
  • Shun-ichi Kuwana
    • 4
  • Yuki Hosokawa
    • 2
  • Eiki Hatori
    • 2
  • Junzo Takeda
    • 2
  1. 1.Department of Physiology and PediatricsUniversity of AlbertaEdmontonCanada
  2. 2.Department of AnesthesiologyKeio University School of MedicineTokyoJapan
  3. 3.Department of AnesthesiologyKitasato Institute HospitalTokyoJapan
  4. 4.Uekusa Gakuen UniversityTokyoJapan

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