Abstract
Biopsy of the first and second polar bodies allows investigation of the chromosomal and genetic constitution of the corresponding oocyte. Opening of the zona pellucida prior biopsy can be performed using a bevelled pipette, by 3D partial zona dissection or by a non-contact diode laser. Acid tyrode solution is not suitable for zona drilling in oocytes. The biopsy procedure through such an opening allows for the simultaneous or sequential removal of the first and/or second polar body. Once biopsied, polar bodies can be analysed by fluorescence in situ hybridization or by array-comparative genomic hybridization for numerical and structural chromosomal aberrations or by polymerase chain reaction-based techniques for monogenetic diseases. Biopsy of the first and second polar bodies has no impact on further embryo development.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Verlinsky Y, Ginsberg N, Lifchez A, et al. Analysis of the first polar body: preconception genetic diagnosis. Hum Reprod. 1990;5:826–9.
Verlinsky Y, Rechitsky S, Cieslak J, et al. Preimplantation diagnosis of single gene disorders by two-step oocyte genetic analysis using first and second polar body. Biochem Mol Med. 1997;62:182–7.
Strom CM, Ginsberg N, Rechitsky S, et al. Three births after preimplantation genetic diagnosis for cystic fibrosis with sequential first and second polar body analysis. Am J Obstet Gynecol. 1998;178:1298–306.
Kuliev A, Rechitsky S, Verlinsky O, et al. Birth of healthy children after preimplantation diagnosis of thalassemias. J Assist Reprod Genet. 1999;16:207–11.
Munné S, Fung J, Cassel MJ, et al. Preimplantation genetic analysis of translocations: case-specific probes for interphase cell analysis. Hum Genet. 1998;102:663–74.
Verlinsky Y, Rechitsky S, Schoolcraft W, et al. Preimplantation diagnosis for Fanconi anemia combined with HLA matching. JAMA. 2001;285:3130–3.
Verlinsky Y, Rechitsky S, Verlinsky O, et al. Polar body-based preimplantation diagnosis for x-linked disorders. Reprod Biomed Online. 2002;4:38–42.
Munné S, Dailey T, Sultan KM, et al. The use of first polar bodies for preimplantation diagnosis of aneuploidy. Hum Reprod. 1995;10:1015–21.
Verlinsky Y, Cieslak J, Freidine M, et al. Pregnancies following pre-conception diagnosis of common aneuploidies by fluorescent in-situ hybridization. Hum Reprod. 1995;10:1923–7.
Verlinsky Y, Tur-Kaspa I, Cieslak J, et al. Preimplantation testing for chromosomal disorders improves reproductive outcome of poor-prognosis patients. Reprod Biomed Online. 2005;11:219–25.
Montag M, van der Ven K, Dorn C, van der Ven H. Outcome of laser-assisted polar body biopsy. Reprod Biomed Online. 2004;9:425–9.
Montag M, Schimming T, van der Ven H. Spindle imaging in human oocytes: the impact of the meiotic cell cycle. Reprod Biomed Online. 2006;12:442–6.
Gordon JW, Talansky BE. Assisted fertilization by zona drilling: a mouse model for correction of oligospermia. J Exp Zool. 1987;239:347–81.
Malter HE, Cohen J. Blastocyst formation and hatching in vitro following zona drilling of mouse and human embryos. Gamete Res. 1989;24:67–80.
Cieslak J, Ivakhenko V, Wolf G, et al. Three-dimensional partial zona dissection for preimplantation genetic diagnosis and assisted hatching. Fertil Steril. 1999;71:308–13.
Rink K, Delacrétaz G, Salathé RP, et al. 1.48 μm diode laser microdissection of the zona pellucida of mouse zygotes. Proc Soc Photo Opt Instrum Eng. 1994;213A:412–22.
Montag M, van der Ven K, Delacrétaz G, et al. Laser assisted microdissection of zona pellucida facilitates polar body biopsy. Fertil Steril. 1998;69:539–42.
Boada M, Carrera M, De La Iglesia C, et al. Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: report of two cases. J Assist Reprod Genet. 1997;15:301–5.
Joris H, De Vos A, Janssens R, et al. Comparison of the results of human embryo biopsy and outcome of PGD after zona drilling using acid Tyrode medium or a laser. Hum Reprod. 2003;18:1896–902.
Montag M, van der Ven K, van der Ven H. Erste klinische Erfahrungen mit der Polkörperdiagnostik in Deutschland. J Fertil Reprod. 2002;4:7–12.
Montag M, van der Ven H. Laser-assisted hatching in assisted reproduction. Croat Med J. 1999;40:398–403.
Montag M, van der Ven K, van der Ven H. Polar body biopsy. In: Gardner D, Weissman A, Howles C, Shoham Z, editors. Textbook of assisted reproductive techniques: laboratory and clinical perspectives. 3rd ed. New York: Informa Healthcare; 2009. p. 357–70.
Geraedts J, Collins J, Gianaroli L, et al. What next for preimplantation genetic screening? A polar body approach! Hum Reprod. 2010;3:575–7.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this chapter
Cite this chapter
Montag, M., Köster, M., van der Ven, K., van der Ven, H. (2012). Polar Body Biopsy. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Practical Manual of In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1780-5_50
Download citation
DOI: https://doi.org/10.1007/978-1-4419-1780-5_50
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4419-1779-9
Online ISBN: 978-1-4419-1780-5
eBook Packages: MedicineMedicine (R0)