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Polar Body Biopsy

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In Vitro Fertilization

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Abstract

Biopsy of the first and second polar bodies allows investigation of the chromosomal and genetic constitution of the corresponding oocyte. Opening of the zona pellucida prior to biopsy can be performed using a bevelled pipette, by three-dimensional partial zona dissection or by a non-contact diode laser. Acid tyrode solution is not suitable for zona drilling in oocytes. The biopsy procedure through such an opening allows for the simultaneous or sequential removal of the first and/or second polar body. Once biopsied, polar bodies can be analysed by a variety of methods for numerical and structural chromosomal aberrations (FISH, fluorescence in situ hybridization; aCGH, array-comparative genomic hybridization; NGS, next-generation sequencing; SNP-array, single nucleotide polymorphism array) or for monogenetic diseases using polymerase chain reaction (PCR)-based methods. Biopsy of the first and second polar bodies has no effect on further embryo development.

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Author information

Authors and Affiliations

  1. ilabcomm GmbH, Sankt Augustin, Germany

    Markus Montag

Authors
  1. Markus Montag
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Correspondence to Markus Montag .

Editor information

Editors and Affiliations

  1. Reproductive Biology Associates – Prelude, Atlanta, GA, USA

    Zsolt Peter Nagy

  2. Astra Fertility Group, Mississauga, ON, Canada

    Alex C. Varghese

  3. Cleveland Clinic, Cleveland, OH, USA

    Ashok Agarwal

Review Questions

Review Questions

  1. 1.

    What kind of equipment is required for polar body biopsy?

  2. 2.

    When is the best time to perform polar body biopsy?

  3. 3.

    How is zona opening best accomplished?

  4. 4.

    How is the biopsy procedure performed?

  5. 5.

    What are potential pitfalls during biopsy?

  6. 6.

    How are polar bodies harvested for later analysis?

  7. 7.

    What is the current status of polar body biopsy?

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Montag, M. (2019). Polar Body Biopsy. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) In Vitro Fertilization. Springer, Cham. https://doi.org/10.1007/978-3-319-43011-9_47

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  • DOI: https://doi.org/10.1007/978-3-319-43011-9_47

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-43010-2

  • Online ISBN: 978-3-319-43011-9

  • eBook Packages: MedicineMedicine (R0)

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