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Regulation of Sciarid DNA Puffs by Ecdysone: Mechanisms and Perspectives

  • Chapter
Ecdysone: Structures and Functions

DNA puffs, formed in the salivary gland polytene chromosomes at the end of the larval stage, are characteristic of Sciaridae. Cytological demonstrations coupled to the molecular characterization of DNA puffs revealed that they are sites of disproportional DNA synthesis and abundant transcription. The biological purpose of DNA amplification at these loci is to enable the synthesis of large amounts of proteins in short periods of time. Here, the role of ecdysone in DNA puffs formation and DNA puff genes amplification and expression is reviewed and a new model for ecdysone action on DNA puffs is proposed. Studies in transgenic Drosophila that contributed to the understanding of the mechanisms that regulate Sciaridae DNA puff gene amplification and transcription are described. Finally, the availability of antibodies raised against the EcR, will further extend the knowledge about the roles exerted by ecdysone in this unique model system.

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Authors and Affiliations

  1. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040–903, Brazil

    Nadia Monesi & Juliana Aparecida Candido-Silva

  2. Departamento de Biologia Celular, Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil

    Maria Luísa Paçó-Larson & Jorge Cury de Almeida

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  1. Nadia Monesi
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  2. Juliana Aparecida Candido-Silva
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  3. Maria Luísa Paçó-Larson
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  4. Jorge Cury de Almeida
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Correspondence to Nadia Monesi .

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  1. Laboratory of Agrozoology Faculty of Bioscience Engineering, Ghent University, Belgium

    Guy Smagghe

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Monesi, N., Candido-Silva, J.A., Paçó-Larson, M.L., de Almeida, J.C. (2009). Regulation of Sciarid DNA Puffs by Ecdysone: Mechanisms and Perspectives. In: Smagghe, G. (eds) Ecdysone: Structures and Functions. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9112-4_6

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