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DNA Fingerprinting Methods for Microbial Pathogens: Application to Diagnostics, Taxonomy and Plant Disease Management

  • Keith R. Mitchelson
  • Salvatore Moricca
Part of the Integrated Management of Plant Pests and Diseases book series (IMPD, volume 3)

Abstract

Advanced molecular genetic techniques enhance our capabilities to identify and characterize microbial pathogens, resulting in accurate testing for pathogen identification, sub-species-level DNA fingerprinting, pathogen-load testing and disease spread monitoring. These applications are instrumental to the study of plant disease epidemiology, so that adequate control measures can be accordingly implemented. In this chapter, a survey of the most popular DNA profiling techniques is presented together with some of the newer and most discriminating molecular methods. Combinations of different analytical techniques are also proposed as a useful approach for low throughput bioassays. Advantages and disadvantages of each single test are taken into account and key issues (sampling, validation, large-scale testing, etc.), encountered in the practical application of these assays, are discussed. An outline of emerging high-throughput molecular technologies expected to improve diagnostic approaches and aid disease management is also provided.

Keywords

Amplify Fragment Length Polymorphism Ribosomal Gene Massively Parallel Signature Sequencing Nucleic Acid Research Strand Displacement Amplification 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Keith R. Mitchelson
    • 1
    • 2
  • Salvatore Moricca
    • 3
  1. 1.National Engineering Research Centre for Beijing Biochip TechnologyChina
  2. 2.The Medical Systems Biology Research Center Tsinghua University School of MedicineChina
  3. 3.Dipartimento di Biotecnologie agrarie Sezione di Patologia vegetaleUniversità di FirenzeItaly

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