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Rapid and efficient extraction of genomic DNA from differentphytopathogenic fungi using DNAzol reagent

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Abstract

A modified procedure using the commercial DNAzol reagent was successfully applied to extract genomic DNA from 25 fungal species. The DNA yield varied from 306 to 1,927 μg g-1 dry mycelia and the A260/A280 ratio from 1.59 to 1.93. Compared with the method of J.L. Cenis (Nucleic Acids Res. 1992, 20: 2380) this procedure generated a higher DNA yield from 17 species and a higher A260/A280 ratio from 23 species. But for four species, Cenis (1992) method was more suitable. No inhibitor of polymerase chain reaction was evident for the DNA extracted by the modified procedure, whereas some inhibitors remained in DNA of eight species extracted by the previous method.

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Authors and Affiliations

  1. Institute of Phytopathology, University of Kiel, Hermann-Rodewald-Str. 9, D-24118, Kiel, Germany

    Jian-Rong Guo, F. Schnieder, K. A. Abd-Elsalam & J. A. Verreet

  2. Plant Pathology Research Institute, Agricultural Research Centre, 9-Gamaa St, Giza, Egypt

    K. A. Abd-Elsalam

Authors
  1. Jian-Rong Guo
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  2. F. Schnieder
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  3. K. A. Abd-Elsalam
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  4. J. A. Verreet
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Corresponding author

Correspondence to Jian-Rong Guo.

Additional information

Revisions requested 8 September 2004; Revisions received 1 November 2004

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Guo, JR., Schnieder, F., Abd-Elsalam, K.A. et al. Rapid and efficient extraction of genomic DNA from differentphytopathogenic fungi using DNAzol reagent. Biotechnol Lett 27, 3–6 (2005). https://doi.org/10.1007/s10529-004-6294-x

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  • DOI: https://doi.org/10.1007/s10529-004-6294-x

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