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Molecular Chaperones and Protection in Animal and Cellular Models of Ischemic Stroke

  • Yi-Bing Ouyang
  • Lijun Xu
  • Rona G. Giffard
Part of the Heat Shock Proteins book series (HESP, volume 3)

Abstract

Chaperones, including heat-shock proteins (Hsps) Hsp70, Hsp40 and GroEL, has been shown to protect from both global and focal ischemia in vivo and cell culture models of ischemia/reperfusion injury in vitro. While the mechanism of protection in part reflects chaperone functions (i.e., preventing abnormal protein folding or aggregation), work from our laboratory and others has implicated additional mechanisms including direct interference with cell death pathways, modulation of inflammation, and preservation of mitochondrial function. In this chapter we will first briefly introduce the Hsps, then describe the animal and cellular models of cerebral ischemia in which effects of Hsps have been studied, including reviewing methods used to overexpress Hsps. We will focus on the protective effect of overexpressing different Hsps against ischemic stroke and elaborate the potential mechanisms involved. Despite a great deal of study, much remains to be learned about the multifaceted effects of Hsps in cerebral ischemia. The endogenous stress response remains a model of cell protection with promise for the development of novel therapies for ischemic brain injury

Keywords

Apoptosis cell culture cerebral ischemia inflammation heat shock proteins neuroprotection 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Yi-Bing Ouyang
    • 1
  • Lijun Xu
    • 1
  • Rona G. Giffard
    • 1
    • 2
  1. 1.Department of AnesthesiaStanford University School of MedicineStanfordUSA
  2. 2.Department of AnesthesiaStanford University School of MedicineStanfordUSA

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