Abstract
Photochemically induced dynamic nuclear polarisation (photo-CIDNP) has been observed at two different magnetic field 9.4 and 4.7 Tesla, in reaction centers (RCs) of green sulphur bacterium Chlorobium (C.) tepidum by 13C magic-angle spinning (MAS) solid-state NMR.The data suggest that the donor is a very symmetric dimer and the electronic coupling parameters are similar to those of RCs of Rhodobacter sphaeroides and of photosystem I.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Alia, Roy E, Gast P, van Gorkom HJ, de Groot HJM, Jeschke G, Matysik J (2004) Photochemically induced dynamic nuclear polarization in photosystem I of plants observed by 13C magic-angle spinning NMR. J Am Chem Soc 126:12819-12826.
Daviso E, Jeschke G, Matysik J (2008) Photo-CIDNP MAS NMR. In: Aartsma TJ, Matysik J (eds) Biophysical Methods in Photosynthesis. Springer, Dordrecht, The Netherlands (in press).
Feiler U, Hauska G (1995) The reaction center from green sulphur bacteria. In: Blankenship RE, Madigan MT, Bauer CE (eds) Anoxygenic Photosynthetic Bacteria. Kluwer, Dordrecht, The Netherlands, pp 665-685.
Frankenberg N, Hager-Braun C, Feiler U, Fuhrmann M, Rogl H, Schneebauer N, Nelson N, Hauska G (1996) P840-reaction centers from Chlorobium tepidum qui-none analysis and functional reconstitution into lipid vesicles. Photochem Photobiol 64:14-19.
Jeschke G, Matysik J (2003) A reassessment of the origin of photochemically induced dynamic nuclear polarization effects in solids. Chem Phys 294:239-255.
Kjaer B, Frigaard NU, Yang F, Zybailov B, Miller M, Golbeck JH, Scheller HV (1998) Menaquinone-7 in the reaction center complex of the green sulfur bacterium Chlorobium vibrioforme functions as the electron acceptor A1. Biochemistry 37:3237-3242.
Kleinherenbrink FAM, Ikegami I, Hiraishi A, Otte SCM, Amesz J (1993) Electron transfer in menaquinone- depleted membranes of Heliobacterium chlorum. Biochim Biophys Acta 1142:69-73.
Kobayashi M, Oh-oka H, Akutsu S, Akiyama M, Tominaga K, Kise H, Nishida F, Watanabe T, Amesz J, Koizumi M, Ishida N, Kano H (2000) The primary electron acceptor of green sulfur bacteria. Photosynth Res 63:269-280.
Matysik J, Alia, Hollander JG, Egorova-Zachernyuk T, Gast P, de Groot HJM (2000) A set-up to study photochemi-cally induced dynamic nuclear polarization in photosyn-thetic reaction centers by solid-state NMR. Indian J Biochem Biophys 37:418-423.
Nitschke W, Feiler U, Lockau W, Hauska G (1987) Photosynthetic reaction centers of green sulfur bacteria studies by EPR. FEBS Lett 218:283-286.
Nuijs AM, Vasmel H, Joppe HLP, Duysens LNM, Amesz J (1985) Excited states and primary charge separation in the pigment system of the green photosynthetic bac-terium Prosthecochloris aestuarii. Biochim Biophys Acta 807:24-34.
Prakash S, Alia, Gast P, de Groot HJM, Jeschke G, Matysik J (2005) Magnetic field dependence of photo-CIDNP MAS NMR on photosynthetic reaction centers in Rhodobacter sphaeroides WT. J Am Chem Soc 127:14290-14298.
Prakash S, Alia, Gast P, de Groot HJM, Matysik J, Jeschke G (2006) Photo-CIDNP MAS NMR in intact cells of Rhodobacter sphaeroides R26: Molecular and atomic resolution at nanomolar concentration. J Am Chem Soc 128:12794-112799.
Roy E, Alia, Gast P, van Gorkom HJ, de Groot HJM, Jeschke G, Matysik J (2007a) Photochemically induced dynamic nuclear polarization in the reaction center of the green sulphur bacterium Chlorobium tepidum observed by 13C MAS NMR. Biochim Biophys Acta 1767:610-615.
Roy E, Diller A, Alia, Gast P, van Gorkom HJ, de Groot HJM, Jeschke G, Matysik J (2007b) Magnetic field dependence of 13C photo-CIDNP MAS NMR in plant photosystems I and II. Appl Magn Reson 31:193-204.
Vassiliev IR, Antonkine ML, Golbeck JH (2001) Iron-sulphur clusters in type I reaction centers. Biochim Biophys Acta 1507:139-160.
Wahlund TM, Woese CR, Castenholz RW, Madigan MT (1991) A theromphilic green sulfur bacterium from New-Zealand hot-springs Chlorobium tepidum. Arch Microbiol 156:81-90.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2008 Springer Science + Business Media, B.V.
About this paper
Cite this paper
Roy, E., Alia, A., Gast, P., van Gorkom, H.J., Jeschke, G., Matysik, J. (2008). 13C Photo-CIDNP MAS NMR on the Reaction Center of Green Sulphur Bacterium at Two Different Magnetic Fields. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_39
Download citation
DOI: https://doi.org/10.1007/978-1-4020-6709-9_39
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6707-5
Online ISBN: 978-1-4020-6709-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)