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Mössbauer- and EPR-Snapshots of an Enzymatic Reaction: The Cytochrome P450 Reaction Cycle

  • Conference paper
ICAME 2003

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Abstract

In this communication we present a complimentary Mössbauer- and EPR-study of the time dependance of the reaction of substrate free P450cam with peracetic acid within a time region ranging from 8 ms up to 5 min. An Fe(IV) species as well as a tyrosyl radical residing on the amino acid residue Tyr96 have been identified as reaction intermediates. These species possibly are formed by the reduction of compound I by means of transferring an electron from Tyr 96 to the heme moiety.

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References

  1. Lewis, D. F. V., Cytochromes P450 — Structure, Function and Mechanism, Taylor and Francis Ltd, London, 1996.

    Google Scholar 

  2. Sharrock, M., Debrunner, P. G., Schulz, C., Lipscomb, J. D., Marshall, V. and Gunsalus, I. C., Biochim. Biophys. Acta 420 (1976), 8.

    Article  Google Scholar 

  3. Poulos, T. L., Finzel, B. C. and Howard, A. J., Biochemistry 25 (1986), 5314.

    Article  Google Scholar 

  4. Rutter, R., Hager, L. P., Dhonau, H., Hendrich, M., Valentine, M. and Debrunner, R, Biochemistry 23 (1984), 6809.

    Article  Google Scholar 

  5. Bill, E., Hyp. Interact. 90 (1994), 143.

    Article  ADS  Google Scholar 

  6. Schünemann, V., Jung, C., Trautwein, A. X., Mandon, D. and Weiss, R., FEBSLett. 479 (2000), 149.

    Article  Google Scholar 

  7. Schünemann, V., Jung, C., Terner, J., Trautwein, A. X. and Weiss, R., J. Inorg. Biochem. 91 (2002), 586.

    Article  Google Scholar 

  8. Unger, B. P., Gunsalus, I. C. and Sligar, S. G., J. Biol. Chem. 262 (1986), 1158.

    Google Scholar 

  9. Atkins, W. M. and Sligar, S. G., J. Biol. Chem. 263 (1988), 18842.

    Google Scholar 

  10. Jung, C., Hui Bon Hoa, G., Schröder, K.-L., Simon, M. and Doucet, J. P., Biochemistry 35 (1992), 12855.

    Article  Google Scholar 

  11. Beinert, H. and Albracht, S., Biochim. Biophys. Acta 683 (1982), 245.

    Article  Google Scholar 

  12. Barra, A. L., Appl. Magn. Res. 21 (2001), 619.

    Article  Google Scholar 

  13. Trautwein, A. X., Bill, E., Bominaar, E. L. and Winkler, H., Structure and Bonding 78 (1991), 1.

    Article  Google Scholar 

  14. Sharrock, M., Debrunner, P. G., Schulz, C., Lipscomb, J. D., Marshall, V. and Gunsalus, I. C., Biochim. Biophys. Acta 420 (1976), 8.

    Article  Google Scholar 

  15. Jung, C., Schünemann, V., Lendzian, F., Contzen, J., Barra, A.-L. and Trautwein, A. X., In: P. Anzenbacher and J. Hudecek (eds), Cytochromes P450, Biochemistry, Biophysics and Drug Metabolism, 13th Internat. Conf. on Cytochromes P450, Pague, 2003, Monduzzi Editore Bologna, p. 201.

    Google Scholar 

  16. Debrunner, R G., In: A. B. R. Lever and H. B. Gray (eds), Iron Porphyrins, Part III, VCH Publishing, Heidelberg, 1989, p. 139.

    Google Scholar 

  17. Schünemann, V., Trautwein, A. X., Jung, C. and Terrer, J., Hyp. Interact. 141/142 (2002), 279.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Physics, University of Lübeck, D-23538, Lübeck, Germany

    V. Schünemann, T. Teschner & A. X. Trautwein

  2. Max-Delbrück-Center for Molecular Medicine, D-13125, Berlin, Germany

    C. Jung

  3. Max-Volmer Laboratory for Biophysical Chemistry, Technical University, PC 14, D-10623, Berlin, Germany

    F. Lendzian

  4. Grenoble High Magnetic Field Laboratory, CNRS, B.P. 166, F-38042, Grenoble Cedex, France

    A.-L. Barra

Authors
  1. V. Schünemann
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  2. C. Jung
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  3. F. Lendzian
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  4. A.-L. Barra
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  5. T. Teschner
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  6. A. X. Trautwein
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Editor information

Editors and Affiliations

  1. Sultan Qaboos University, Muscat, Oman

    M. E. Elzain , A. A. Yousif , A. D. al Rawas  & A. M. Gismelseed , ,  & 

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Schünemann, V., Jung, C., Lendzian, F., Barra, AL., Teschner, T., Trautwein, A.X. (2004). Mössbauer- and EPR-Snapshots of an Enzymatic Reaction: The Cytochrome P450 Reaction Cycle. In: Elzain, M.E., Yousif, A.A., al Rawas, A.D., Gismelseed, A.M. (eds) ICAME 2003. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2852-6_38

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  • DOI: https://doi.org/10.1007/978-1-4020-2852-6_38

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6726-5

  • Online ISBN: 978-1-4020-2852-6

  • eBook Packages: Springer Book Archive

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