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Fluorescence Techniques in Biomedicine: From the Monitoring of Cell Metabolism to Image Processing in Cancer Detection

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Reviews in Fluorescence 2004

Part of the book series: Reviews in Fluorescence 2004 ((RFLU,volume 2004))

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Abstract

The state of the art in fluorescence imaging for biomedical applications is demonstrated. 2-dimensional profiles of fluorophores gained with non-contact techniques show the quantitative distribution of endogenous NADH in the UV and synthetic markers in the NIR spectral range. The biomedical use extends from basic research investigations on the metabolism in mitochondria to clinical applications when differentiating the tumor border zone.

One of the outstanding advantages of near infrared so-called Optical Molecular Imaging (OMI) is bright fluorescence of the markers by specific molecular interaction with tumor specific enzymes. For the in vivo tests of the dyes an experimental NIR imager was used. NIR fluorescence of the entire body of small animals can be imaged.

The analysis of fluorescences from the interior of the probes shows strong intensity distortion due to tissue optics. Rescaling as the physical basis for image processing taking into account biochemical and biooptical methods result in the real concentration of the fluorophore under consideration. For example, the diameter of the fluorescent volume is apparently larger without rescaling. This new interpretation of fluorescence pictures has useful applications in biomedicine now and in the future.

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Authors and Affiliations

  1. University Hospital Benjamin Franklin, Institute for Medical Physics, Freie Universität Berlin, Fabeckstr. 60-62, 14195, Berlin, Germany

    Olaf Minet & Jürgen Beuthan

  2. Institute for Biomedical Research, Kaunas University of Medicine, Eiveniu 4, LT-3007, Kaunas, Lithuania

    Vida Mildažiene & Rasa Baniene

Authors
  1. Olaf Minet
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  2. Jürgen Beuthan
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  3. Vida Mildažiene
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  4. Rasa Baniene
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Editors and Affiliations

  1. Institute of Fluorescence, University of Maryland Biotechnology Institute, Baltimore, Maryland, USA

    Chris D. Geddes

  2. Center for Fluorescence Spectroscopy, University of Maryland, Baltimore, Maryland, USA

    Joseph R. Lakowicz

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Minet, O., Beuthan, J., Mildažiene, V., Baniene, R. (2004). Fluorescence Techniques in Biomedicine: From the Monitoring of Cell Metabolism to Image Processing in Cancer Detection. In: Geddes, C.D., Lakowicz, J.R. (eds) Reviews in Fluorescence 2004. Reviews in Fluorescence 2004, vol 2004. Springer, Boston, MA. https://doi.org/10.1007/978-0-306-48672-2_10

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  • DOI: https://doi.org/10.1007/978-0-306-48672-2_10

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