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Insights into chloroplast proteomics: from basic principles to new horizons

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Cell and Molecular Biology of Plastids

Part of the book series: Topics in Current Genetics ((TCG,volume 19))

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Abstract

Many proteomic approaches have been employed to investigate the complex and dynamic proteomeof the chloroplast. These range from classical methods like one and two dimensional gel electrophoresisto advanced comparative proteomics strategies such as ICAT or SILAC. Mass spectrometry for proteinidentification or quantitation plays an important role in most of the methods used and is a fastemerging technology in protein biochemistry. Most proteomic studies of the chloroplast focus on thesingle compartments of this plant organelle, which greatly reduces the complexity of the sample andthus allows for a more complete and detailed analysis of the complex protein composition. Therapidly developing field of comparative proteomics makes it possible to analyze dynamic protein changescaused, for example, by different developmental stages of a plant, by various stress conditionsand distinct genetic backgrounds.

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  1. Institute of Plant Biochemistry and Biotechnology, University of Muenster, Hindenburgplatz 55, 48143, Muenster, Germany

    Bianca Naumann & Michael Hippler

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  1. Bianca Naumann
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  2. Michael Hippler
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Naumann, B., Hippler, M. (2007). Insights into chloroplast proteomics: from basic principles to new horizons. In: Bock, R. (eds) Cell and Molecular Biology of Plastids. Topics in Current Genetics, vol 19. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4735_2007_0224

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