Abstract
We have characterized the hydrodynamic behaviour in H 2 O and D2O of two detergents, dodecyl-β-D-maltoside (DDM) and octaethylene glycol monododecyl ether (C12E8), by analysing sedimentation velocity profiles obtained with interference optics in terms of continuous particle distribution and non-interacting species. The analysis in H20 provides values for the sedimentation and diffusion coefficients that give aggregation numbers of 130 and 115 for DDM and C12E8, respectively, close to that given in the literature. The analysis of the number of interference fringes as a function of detergent concentration gave values for the critical micelle concentration and refractive index increments that are also in good agreement with published values. The values of the partial specific volumes of DDM and C12E8, obtained by sedimentation in H2O and D2O – where C12E8 floats – are identical to those obtained from density measurements. These results indicate that sedimentation velocity has good potential for proper characterisation of detergent-solubilized proteins.
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Chabre M, Cone R, Saibil H (2003) Nature 426:30
Fotiadis D, Liang Y, Filipek S, Saperstein DA, Engel A, Palczewski K (2003) Nature 421:127
Jastrzebska B, Maeda T, Zhu L, Fotiadis D, Filipek S, Engel A, Stenkamp RE, Palczewski K (2004) J Biol Chem 279:54663
Medina R, Perdomo D, Bubis J (2004) J Biol Chem 279:39565
Ubarretxena-Belandia I, Baldwin JM, Schuldiner S, Tate CG (2003) Embo J 22:6175
Butler PJ, Ubarretxena-Belandia I, Warne T, Tate CG (2004) J Mol Biol 340:797
Gottschalk KE, Soskine M, Schuldiner S, Kessler H (2004) Biophys J 86:3335
Ma C, Chang G (2004) Proc Natl Acad Sci USA 101:2852
Winstone TL, Jidenko M, le Maire M, Ebel C, Duncalf KA, Turner RJ (2005) Biochem Biophys Res Commun 327:437
Moller JV, le Maire M (1993) J Biol Chem 268:18659
le Maire M, Champeil P, Moller JV (2000) Biochim Biophys Acta 1508:86
Cohen E, Goldshleger R, Shainskaya A, Tal DM, Ebel C, le Maire M, Karlish SJ (2005) J Biol Chem 280:16610
Stanley AM, Fleming KG (2005) J Mol Biol 347:759
Fleming KG, Ackerman AL, Engelman DM (1997) J Mol Biol 272:266
Fleming KG, Ren CC, Doura AK, Eisley ME, Kobus FJ, Stanley AM (2004) Biophys Chem 108:43
Sturgis JN, Robert B (1994) J Mol Biol 238:445
Fisher LE, Engelman DM, Sturgis JN (2003) Biophys J 85:3097
Josse D, Ebel C, Stroebel D, Fontaine A, Borges F, Echalier A, Baud D, Renault F, Le Maire M, Chabrieres E, Masson P (2002) J Biol Chem 277:33386
DeGrado WF, Gratkowski H, Lear JD (2003) Protein Sci 12:647
Popot JL, Engelman DM (1990) Biochemistry 29:4031
Engelman DM, Chen Y, Chin CN, Curran AR, Dixon AM, Dupuy AD, Lee AS, Lehnert U, Matthews EE, Reshetnyak YK, Senes A, Popot J-L (2003) FEBS Lett 555:122
Ebel C (2004) Progr Colloid Polym Sci 127:73
Lebowitz J, Lewis MS, Schuck P (2002) Protein Sci 11:2067
Rivas G, Stafford W, Minton AP (1999) Methods 19:194
Schuck P (1998) Biophys J 75:1503
Demeler B, Behlke J, Ristau O (2000) Methods Enzymol 321:38
Philo JS (2000) Anal Biochem 279:151
Stafford WF (2000) Methods Enzymol 323:302
Stafford WF, Sherwood PJ (2004) Biophys Chem 108:231
Dam J, Velikovsky CA, Mariuzza RA, Urbanke C, Schuck P (2005) Biophys J 89:619
Dam J, Schuck P (2005) Biophys J 89:651
Solovyova A, Schuck P, Costenaro L, Ebel C (2001) Biophys J 81:1868
Schuck P (2000) Biophys J 78:1606
Schuck P, Perugini MA, Gonzales NR, Howlett GJ, Schubert D (2002) Biophys J 82:1096
Dam J, Schuck P (2004) Methods Enzymol 384:185
Edelstein SJ, Schachman HK (1973) Methods Enzymol 27:82
Noy D, Calhoun JR, Lear JD (2003) Anal Biochem 320:185
Gohon Y, Pavlov G, Timmins P, Tribet C, Popot J-L, Ebel C (2004) Anal Biochem 334:318
Balbo A, Minor KH, Velikovsky CA, Mariuzza RA, Peterson CB, Schuck P (2005) Proc Natl Acad Sci USA 102:81
Tiefenbach K-J, Durchschlag H, Jaenicke J (1999) Progr Colloid Polym Sci 113:135
Hayashi Y, Matsui H, Takagi T (1989) Methods Enzymol 172:514
Acknowledgments
This work was supported by the CNRS (postdoctoral fellowship to Andrès Salvay), CEA and Université Joseph Fourrier, Grenoble, France.
Author information
Authors and Affiliations
Corresponding author
Editor information
Rights and permissions
About this paper
Cite this paper
Salvay, A.G., Ebel, C. Analytical Ultracentrifuge for the Characterization of Detergent in Solution. In: Wandrey, C., Cölfen, H. (eds) Analytical Ultracentrifugation VIII. Progress in Colloid and Polymer Science, vol 131. Springer, Berlin, Heidelberg. https://doi.org/10.1007/2882_006
Download citation
DOI: https://doi.org/10.1007/2882_006
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-29615-7
Online ISBN: 978-3-540-34279-3
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)