Computational Design Approach to Hydrodynamic Focusing in a Flow Cytometer
A two-fluid theoretical model was developed to simulate the hydrodynamic focusing process formed by the coflowing sample and sheath fluids in a flow cytometer. The analysis consists of two groups of time-dependent three-dimensional conservation equations of mass and momentum. To validate the computer code, the predicted focused width in the two-dimensional test configuration was compared with Lee et al.’s measured data. The present study also examines the pressure distribution of the three-dimensional hydrodynamic focusing flowfield. For the u Sh /u S ratio ranging from 10 to 80, the focused width was determined to explore the applicability of the proposed flow cytometer.
KeywordsFlow Cytometer Sample Fluid Numerical Heat Transfer Sheath Flow Sheath Fluid
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