Computational Design Approach to Hydrodynamic Focusing in a Flow Cytometer

  • An-Shik Yang
  • Chun-Yao Wu
Conference paper
Part of the Lecture Notes in Computer Science book series (LNCS, volume 4115)


A two-fluid theoretical model was developed to simulate the hydrodynamic focusing process formed by the coflowing sample and sheath fluids in a flow cytometer. The analysis consists of two groups of time-dependent three-dimensional conservation equations of mass and momentum. To validate the computer code, the predicted focused width in the two-dimensional test configuration was compared with Lee et al.’s measured data. The present study also examines the pressure distribution of the three-dimensional hydrodynamic focusing flowfield. For the u Sh /u S ratio ranging from 10 to 80, the focused width was determined to explore the applicability of the proposed flow cytometer.


Flow Cytometer Sample Fluid Numerical Heat Transfer Sheath Flow Sheath Fluid 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • An-Shik Yang
    • 1
  • Chun-Yao Wu
    • 1
  1. 1.Department of Mechanical and Automation EngineeringDa Yeh UniversityChang HwaTaiwan

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