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Plastic microchip flow cytometer based on 2- and 3-dimensional hydrodynamic flow focusing

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Abstract

This paper deals with design and fabrication of 2- and 3- dimensional microchip type flow cytometers hydrodynamically driven. Flow rate and pressure distrubution have been calculated from Poiseuille flow theory and results were confirmed by computational fluid dynamics. The flow focusing has been evaluated in 2- and 3-dimensional microchip type flow cytometers fabricated with Polydimetylsiloxane molding process. The shape of focused flow was detected with a laser scanning microscope and favourably compared with calculations based on Poiseuille flow assumptions and CFD (Computational Fluid Dynamics) simulation. Simulation and calculation results showed good correlations to each other, and also to the visualized shape of focused flow in microchip. The 3-dimensional focused flow showed the more stable focusing status in laser induced fluorescence measurements than that in 2-dimensional case.

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Authors and Affiliations

  1. School of Mechanical and Aerospace Engineering, Seoul National University, Shinlim-dong, Kwanak-gu, Seoul, 151-742, Korea

    S. Chung, S. J. Park & D. C. Han

  2. Digital Bio Technology, Co., Rm. 1304, Bldg 136, Seoul National University, Shinlim-dong, Kwanak-gu, Seoul, 151-742, Korea

    J. K. Kim & C. Chung

  3. School of Electrical Engineering and Computer Science, Seoul National University, Seoul, Korea Shinlim-dong, Kwanak-gu, Seoul, 151-742, Korea

    J. K. Chang

Authors
  1. S. Chung
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  2. S. J. Park
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  3. J. K. Kim
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  4. C. Chung
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  5. D. C. Han
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  6. J. K. Chang
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Corresponding author

Correspondence to J. K. Chang.

Additional information

The fabrication of the mold for PDMS microchip was done with the help of K.C. Shin and replica molding processes with the help of K.M. Yi in Digital Bio Technology Co. The authors wish to acknowledge that this paper is a result of the research accomplished with the financial support of the Intelligent Microsystem Center, Seoul, Korea, which is carrying out one of the 21st century’s New Frontier R&D Projects sponsored by the Korea Ministry of Science & Technology.

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Chung, S., Park, S., Kim, J. et al. Plastic microchip flow cytometer based on 2- and 3-dimensional hydrodynamic flow focusing. Microsystem Technologies 9, 525–533 (2003). https://doi.org/10.1007/s00542-003-0302-2

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  • DOI: https://doi.org/10.1007/s00542-003-0302-2

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