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Isolation and Cultivation of Alkaliphiles

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Alkaliphiles in Biotechnology

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 172))

Abstract

Alkaliphilic microorganisms are ubiquitous and inhabit various econiches on Earth. Alkaline environments suitable for alkaliphilic microbial communities may be created by certain geological processes or human activities. Moreover, a significant contribution to the emergence of alkaline conditions may be due to the activity of neutralophilic microorganisms through certain reactions, which explains the widespread distribution of alkaliphiles. Alkaliphilic microorganisms are part of extremophiles and become interesting and useful in environmental and industrial microbiology. With increasing knowledge of alkaliphiles, we greatly increase their biotechnological and industrial application potential. New microorganisms from natural habitats serve as a practically endless source of new enzymes. This chapter summarizes the scattered data on alkaliphiles isolated from habitats other than soda lakes. The conditions for occurrence of alkaline habitats are also considered. Moreover, the chapter reviews some important features on preparation of media for the isolation and cultivation of alkaliphiles. The chapter also includes relevant information on chromatographic analysis of alkaliphiles culture.

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Abbreviations

FID:

Flame ionization detector

GLC:

Gas-liquid chromatography

HPLC:

High-performance liquid chromatography

OTU:

Operational taxonomic unit

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Acknowledgments

This work was partially supported by a grant No. 18-04-00236 from the Russian Foundation for Basic Research “Degradation of nitrogen-containing components of a bacterial cell by alkaliphilic microorganisms of soda lakes” and by Basic Research Program No 17, Subprogram 2 of the Russian Academy of Sciences “Evolution of organic world and planetary processes.”

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  1. Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia

    Vadim V. Kevbrin

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    Gashaw Mamo

  2. Department of Biotechnology, Lund University, Lund, Sweden

    Bo Mattiasson

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Kevbrin, V.V. (2019). Isolation and Cultivation of Alkaliphiles. In: Mamo, G., Mattiasson, B. (eds) Alkaliphiles in Biotechnology. Advances in Biochemical Engineering/Biotechnology, vol 172. Springer, Cham. https://doi.org/10.1007/10_2018_84

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