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Evaluation of Biocompatibility Using In Vitro Methods: Interpretation and Limitations

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Tissue Engineering III: Cell - Surface Interactions for Tissue Culture

Part of the book series: Advances in Biochemical Engineering Biotechnology ((ABE,volume 126))

Abstract

The in vitro biocompatibility of novel materials has to be proven before a material can be used as component of a medical device. This must be done in cell culture tests according to internationally recognized standard protocols. Subsequently, preclinical and clinical tests must be performed to verify the safety of the new material and device. The present chapter focuses on the first step, the in vitro testing according to ISO 10993-5, and critically discusses its limited significance. Alternative strategies and a brief overview of activities to improve the current in vitro tests are presented in the concluding section.

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Acknowledgments

The present work was supported by the European Community’s 7th Framework Programme under grant agreement nos. NMP3-LA-2008-214685 (project Magister) and NMP3-LA-2008-213939 (project POCO).

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Authors and Affiliations

  1. Laboratory for Materials – Biology Interactions, Empa – Materials Science and Technology, Lerchenfeldstasse 5, CH-9014 St, Gallen, Switzerland

    Arie Bruinink

  2. Chemistry and Biology, RMS Foundation, Bischmattstrasse 12, CH-2544, Bettlach, Switzerland

    Reto Luginbuehl

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  1. Arie Bruinink
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  2. Reto Luginbuehl
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Correspondence to Arie Bruinink .

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Editors and Affiliations

  1. Department für Biotechnologie, Universität für Bodenkultur, Muthgasse 18, Wien, 1190, Austria

    Cornelia Kasper

  2. , CrossBIT - Center for Biocompatibility, Hannover Medical School, Feodor-Lynen-Str. 31, Hannover, 30625, Germany

    Frank Witte

  3. , Bioprozess- und Biosystemtechnik, Technische Universität Hamburg-Harburg, Denickestrasse 15, Hamburg, 21073, Germany

    Ralf Pörtner

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Bruinink, A., Luginbuehl, R. (2011). Evaluation of Biocompatibility Using In Vitro Methods: Interpretation and Limitations. In: Kasper, C., Witte, F., Pörtner, R. (eds) Tissue Engineering III: Cell - Surface Interactions for Tissue Culture. Advances in Biochemical Engineering Biotechnology, vol 126. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2011_111

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