Abstract
The requirement for both sterility and the avoidance of dehydration in plant tissue cultures can impose sealing requirements that severely limit the rate of gas exchange in and out of the culture vessel. Conditions within the culture vessel, such as the depth of any water cover, the presence of gelling agents, the bulk and porosity of the tissue and the temperature, also strongly influence in vitro rates of gas exchange, primarily driven by diffusion. This article uses elements of Fick’s Law of Diffusion to identify key factors limiting gas exchange between a culture and its immediate surroundings. In particular, it identifies static liquid media, gelling agents, large tissue mass and warm temperatures as imposing severe limits on diffusive flux for gases such as O2, CO2 and ethylene. The principal barrier to diffusive exchange of gases between the in vitro and ex vitro atmospheres is the wall of the enclosing vessel. This is invariably made of glass or plastic that is gas-impermeable and well-sealed against evaporative drying or entry of micro-organisms. Cultures enclosed in this way will, inevitably, asphyxiate unless a compensating pathway for diffusive gas exchange is contrived or replaced by some system of convective flow that carries gases to and from the tissue. Supplementing diffusive aeration with convective flow is the basis of most successful hydroponics systems for whole plants and may be a prerequisite for securing levels of aeration suitable for autotrophic cultures. The paramount consideration is the extent towhich the total rate of consumption or production of a particular gas by the cultured tissues is matched by the maximum rates of gas transport imposed by the culture itself, its immediate surroundings and the ventilation and sealing system of the culture enclosure.
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References
Afreen F, Zobayed SMA & Kozai T (2002) Photoautotrophic culture of Coffea arabusta somatic embryos: development of a bioreactor for large scale plantlet conversion from cotyledonary embryos. Annals of Botany 90, 21–29
Armstrong W (1979) Aeration in higher plants. Advances in Botanical Research 7: 225–332
Armstrong J, Armstrong W & Beckett PM (1992) Phragmites australis: Venturi-induced and humidity-induced pressure flows enhance rhizome aeration and rhizosphere oxidation. The New Phytologist: 120: 197–207
Armstrong W, Beckett PM, Justin SHFW & Lythe S (1991) Modelling, and other aspects of root aeration by diffusion. In: Jackson MB, Davies DD & Lambers H (eds) Plant Life under Oxygen Deprivation: Ecology, Physiology and Biochemistry (pp. 267–282). SPB Academic, The Hague
Armstrong J, Lemos EEP, Zobayed SMA, Justin SHFW & Armstrong W (1997) A humidity-induced convective throughflow and ventilation system benefits Annona sqamosa L. explants and coconut calloid. Annals of Botany 79: 31–40
Armstrong W, Cousins D, Armstrong J, Turner DW & Beckett PM (2000) Oxygen distribution in wetland plant roots and permeability barriers to gas exchange with the rhizosphere: a microelectrode and modelling study with Phragmites australis. Annals of Botany 86: 687–703
Asplund PT & Curtis WR (2001) Intrinsic oxygen use kinetics of transformed plant root culture. Biotechnology Progress 17: 481–489
Barry-Etienne D, Bertrand B, Vasquez N & Etienne H (2002) Comparison of somatic embryogenesis-derived coffee (Coffea arabica L.) plantlets regenerated in vitro and ex vitro: morphological, mineral and water characteristics. Annals of Botany 90: 77–85
Blackwell PS & Wells EA (1983) Limiting oxygen flux densities for oat root extension. Plant Soil 73: 129–139
Barrett-Lennard EG & Dracup MA (1988) A porous agar medium for improving the growth of plants under sterile conditions. Plant and Soil 108: 294–298
Firn RD, Sharma N & Digby J (1994) Physiology, growth and development of plants and cells in culture — the way ahead. In: Lumsden PJ, Nicholas JR & Davies WJ (eds) Physiology, Growth and Developments of Plants in Culture (pp. 409–421). Kluwer Academic Publishers, Dordrecht
Haupt-Herting S & Fock HP (2002) Oxygen exchange in relation to carbon assimilation in water-stressed leaves during photosynthesis. Annals of Botany 89: 851–859
Jackson MB (1980) Aeration in the nutrient film technique of glasshouse crop production and the importance of oxygen, ethylene and carbon dioxide. Acta Hortic. 98: 61–78
Jackson MB (1990) Hormones and developmental change in plants subjected to submergence or soil waterlogging. Aquatic Botany 39: 49–72
Jackson MB, Blackwell PS, Chrimes JR & Sims TV (1984) Poor aeration in NFT and a means for its improvement. J. Hort. Sci. 59: 439–448
Jackson MB, Abbott AJ, Belcher AR & Hall KC (1987) Gas exchange in plant tissue cultures. In: Jackson MB, Mantell SH & Blake J (eds) Advances in the Chemical Manipulation of Plant Tissue Cultures Monograph No. 16 (pp. 57–72). British Plant Growth Regulator Group, Bristol
Jackson MB, Abbott A, Belcher AR, Hall KC, Butler R & Cameron J (1991) Ventilation in plant tissue cultures and effects of poor aeration on ethylene and carbon dioxide accumulation, oxygen depletion and explant development. Annals of Botany 67: 229–237
Jackson MB, Belcher AR & Brain P (1994) Measuring shortcomings in tissue culture aeration and their consequences for explant development. In: Lumsden PJ, Nicholas JR & Davies WJ (eds) Physiology, Growth and Developments of Plants in Culture (pp. 191–203). Kluwer Academic Publishers, Dordrecht
Poorter H, Vanderwerf A, Atkin OK & Lambers H (1991) Respiratory energy-requirements of roots vary with the potential growth-rate of a plant-species. Physiologia Plantarum 83: 469–475
Righetti B, Magnanini E, Infante R & Predieri S (1990) Ethylene, ethanol, acetaldehyde and carbon dioxide released by Prunus avium shoot cultures. Physiologia Plantarum 78: 507–510
Sakihama Y, Nakamura S & Yamasaki H (2002) Nitric oxide production mediated by nitrate reductase in the green alga Chlamydomonas reinhardtii: an alternative NO pathway in photosynthetic organisms. Plant and Cell Physiology 43: 290–297
Sisler EC, Serek M & Dupille E (1996) Comparison of cyclopropene, 1-methylcyclopropene and 3.3-dimethylcyclopropene as ethylene antagonists in plants. Plant Growth Regulation 18: 169–174
Thorpe TA (2000) History of plant cell culture In: Smith RH (ed) Plant Cell Tiss. Cult. Techniques and Experiments (pp. 1–32). Academic Press, London
Vartapetian BB & Jackson MB (1997) Plant adaptation to anaerobic stress. Annals of Botany 79(Supplement A): 3–20
Verslues PE, Ober ES & Sharp RE (1998) Root growth and oxygen relations at low water potentials. Impact of oxygen availability in polyethylene glycol solutions. Plant Physiology 116: 403–1412
Voesenek LACJ & Blom CWPM (1999) Stimulated shoot elongation: a mechanism of semiaquatic plants to avoid submergence stress. In: Lerner HR (ed) Plant Responses to Environmental Stresses: from Phytohormones to Genome Reorganization (pp. 431–448). Marcel Dekker, New York
Zobayed SMA, Armstrong J & Armstrong W (2001) Micropropagation of potato: evaluation of closed, diffusive and forced ventilation on growth and tuberization. Annals of Botany 87: 53–59
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Jackson, M.B. (2005). Aeration stress in plant tissue cultures. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_35
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DOI: https://doi.org/10.1007/1-4020-3200-5_35
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3199-1
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