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Nitrogen Fixation in Heterocyst-Forming Cyanobacteria

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Genetics and Regulation of Nitrogen Fixation in Free-Living Bacteria

Part of the book series: Nitrogen Fixation: Origins, Applications, and Research Progress ((NITR,volume 2))

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Referemces

  • Adams, D. G. (1992). The effect of Dl-7 azatryptophan on heterocyst development in the cyanobacterium Anabaena cylindrica. J. Gen. Microbiol., 138, 355–362.

    CAS  Google Scholar 

  • Adams, D. G., and Carr, N. G. (1981). Heterocyst differentiation and cell division in the cyanobacterium Anabaena cylindrica: effect of high light intensity. J. Cell Sci., 49, 341–352.

    CAS  PubMed  Google Scholar 

  • Adams, D. G., and Carr, N. G. (1989). Control of heterocyst development in the cyanobacterium Anabaena cylindrica. J. Gen. Microbiol., 135, 839–850.

    CAS  Google Scholar 

  • Adams, D. G. and Duggan, P. S. (1999). Heterocyst and akinete differentiation in cyanobacteria. New Phytol., 144, 3–33.

    Article  Google Scholar 

  • Allen, R. M., Madden, M. S., Ludden, P. W., and Shah, V. K. (1994). Biosynthesis of the iron-molybdenum cofactor of nitrogenase. Crit. Rev. Biotech., 14, 225–249.

    CAS  Google Scholar 

  • Angeloni, S., and Potts, M. (1994). Analysis of the sequences within and flanking the cyanoglobin-encoding gene, glbN, of the cyanobacterium Nostoc commune UTEX 584. Gene, 146, 133–134.

    Article  CAS  PubMed  Google Scholar 

  • Arcondeguy, T., Jack, R., and Merrick, M. (2001). PII signal transduction proteins: pivotal players in microbial nitrogen control. Microbiol. Mol. Biol. Rev., 65, 80–105.

    CAS  PubMed  Google Scholar 

  • Arnold, W., Rump, A., Klipp, W., Priefer, U. B., and Pühler, A. (1988). Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire nitrogen fixation gene cluster of Klebsiella pneumoniae. J. Mol. Biol., 203, 715–738.

    Article  CAS  PubMed  Google Scholar 

  • Axelsson, R., and Lindblad, P. (2002). Transcriptional regulation of Nostoc hydrogenases: Effects of oxygen, hydrogen, and nickel. Appl. Environ. Microbiol., 68, 444–447.

    Article  CAS  PubMed  Google Scholar 

  • Axelsson, R., Oxelfelt, F., and Lindblad, P. (1999). Transcriptional regulation of Nostoc uptake hydrogenase. FEMS Microbiol. Lett., 170, 77–81.

    CAS  PubMed  Google Scholar 

  • Baier, K., Nicklisch, S., Grundner, C., Reinecke, J., and Lockau, W. (2001). Expression of two nblA-homologous genes is required for phycobilisome degradation in nitrogen-starved Synechocystis sp. PCC6803. FEMS Microbiol. Lett., 195, 35–39.

    CAS  PubMed  Google Scholar 

  • Bauer, C. C., Ramaswamy, K. S., Endley, S., Scappino, L. A., Golden, J. W., and Haselkorn, R. (1997). Suppression of heterocyst differentiation in Anabaena PCC 7120 by a cosmid carrying wild-type genes encoding enzymes for fatty acid synthesis. FEMS Microbiol. Lett., 151, 23–30.

    CAS  PubMed  Google Scholar 

  • Bishop, P. E., and Joerger, R. D. (1990). Genetics and molecular biology of alternative nitrogen fixation systems. Ann. Rev. Plant Physiol. Plant Mol. Biol., 41, 109–125.

    CAS  Google Scholar 

  • Bishop, P. E., and Premakumar, R. (1992). Alternative nitrogen fixation systems. In G. Stacey, R. H. Burris and H. J. Evans (Eds.), Biological nitrogen fixation (pp. 736–762). New York: Chapman and Hall.

    Google Scholar 

  • Black, K., Buikema, W., and Haselkorn, R. (1995). The hglK gene is required for localization of heterocyst-specific glycolipids in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol., 177, 6440–6448.

    CAS  PubMed  Google Scholar 

  • Black, T. A., Cai, Y., and Wolk, C. P. (1993). Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol. Microbiol., 9, 77–84.

    CAS  PubMed  Google Scholar 

  • Black, T. A., and Wolk, C. P. (1994). Analysis of a Het-mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing. J. Bacteriol., 176, 2282–2292.

    CAS  PubMed  Google Scholar 

  • Boison, G., Bothe, H., and Schmitz, O. (2000). Transcriptional analysis of hydrogenase genes in the cyanobacteria Anacystis nidulans and Anabaena variabilis monitored by RT-PCR. Curr. Microbiol., 40, 315–21.

    Article  CAS  PubMed  Google Scholar 

  • Brahamsha, B., and Haselkorn, R. (1991). Isolation and characterization of the gene encoding the principal sigma factor of the vegetative cell RNA polymerase from the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol., 173, 2442–2450.

    CAS  PubMed  Google Scholar 

  • Brahamsha, B., and Haselkorn, R. (1992). Identification of multiple RNA polymerase sigma factor homologs in the cyanobacterium Anabaena sp. strain PCC 7120: cloning, expression, and inactivation of the sigB and sigC genes. J. Bacteriol., 174, 7273–7282.

    CAS  PubMed  Google Scholar 

  • Brusca, J. S., Hale, M. A., Carrasco, C. D., and Golden, J. W. (1989). Excision of an 11-kilobase-pair DNA element from within the nifD gene in Anabaena variabilis heterocysts. J. Bacteriol., 171, 4138–4145.

    CAS  PubMed  Google Scholar 

  • Bryce, T., Welti, D., Walsby, A., and Nichols, B. (1972). Monohexoside derivatives of long-chain polyhydroxy alcohols; a novel class of glycolipids specific to heterocystous algae. Phytochem., 11, 295–302.

    Article  CAS  Google Scholar 

  • Buikema, W. J., and Haselkorn, R. (1991). Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120. Genes Develop., 5, 321–330.

    CAS  PubMed  Google Scholar 

  • Buikema, W. J., and Haselkorn, R. (1993). Molecular genetics of cyanobacterial development. Ann. Rev. Plant Physiol., 44, 33–52.

    CAS  Google Scholar 

  • Buikema, W. J., and Haselkorn, R. (2001). Expression of the Anabaena hetR gene from a copperregulated promoter leads to heterocyst differentiation under repressing conditions. Proc. Natl. Acad. Sci. U.S.A., 98, 2729–2734.

    Article  CAS  PubMed  Google Scholar 

  • Cai, Y., and Wolk, C. P. (1997). Nitrogen deprivation of Anabaena sp. strain PCC 7120 elicits rapid activation of a gene cluster that is essential for uptake and utilization of nitrate. J. Bacteriol., 179, 258–266.

    CAS  PubMed  Google Scholar 

  • Callahan, S. M., and Buikema, W. J. (2001). The role of HetN in maintenance of the heterocyst pattern in Anabaena sp. PCC 7120. Mol. Microbiol., 40, 941–950.

    Article  CAS  PubMed  Google Scholar 

  • Campbell, E. L., Cohen, M. F., and Meeks, J. C. (1997). A polyketide-synthase-like gene is involved in the synthesis of heterocyst glycolipids in Nostoc punctiforme strain ATCC 29133. Arch. Microbiol., 167, 251–258.

    Article  CAS  PubMed  Google Scholar 

  • Campbell, E. L., Hagen, K. D., Cohen, M. F., Summers, M. L., and Meeks, J. C. (1996). The devR gene product is characteristic of receivers of two-component regulatory systems and is essential for heterocyst development in the filamentous cyanobacterium Nostoc sp. strain ATCC 29133. J. Bacteriol., 178, 2037–2043.

    CAS  PubMed  Google Scholar 

  • Cardemil, L., and Wolk, C. P. (1976). The polysaccharides from heterocyst and spore envelopes of a blue green alga. Methylation analysis and structure of the backbones. J. Biol. Chem., 251, 2967–2975.

    CAS  PubMed  Google Scholar 

  • Cardemil, L., and Wolk, C. P. (1979). The polysaccharides from heterocyst and spore envelopes of a blue-green alga. Structure of the basic repeating unit. J. Biol. Chem., 254, 736–741.

    CAS  PubMed  Google Scholar 

  • Cardemil, L., and Wolk, C. P. (1981a). Isolated heterocysts of Anabaena variabilis synthesize envelope polysaccharide. Biochim. Biophys. Acta, 674, 265–276.

    CAS  PubMed  Google Scholar 

  • Cardemil, L., and Wolk, C. P. (1981b). Polysaccharides from the envelopes of heterocysts and spores of the blue-green algae Anabaena variabilis and Cylindrospermum licheniforme. J. Phycol., 17, 234–240.

    Article  CAS  Google Scholar 

  • Carrasco, C. D., Buettner, J. A., and Golden, J. W. (1995). Programmed DNA rearrangement of a cyano-bacterial hupL gene in heterocysts. Proc. Natl. Acad. Sci. U.S.A., 92, 791–795.

    CAS  PubMed  Google Scholar 

  • Chapman, J. S., and Meeks, J. C. (1983). Glutamine and glutamate transport by Anabaena variabilis. J. Bacteriol., 156, 122–129.

    CAS  PubMed  Google Scholar 

  • Collier, J. L., and Grossman, A. R. (1994). A small polypeptide triggers complete degradation of light-harvesting phycobiliproteins in nutrient-deprived cyanobacteria. EMBO J., 13, 1039–1047.

    CAS  PubMed  Google Scholar 

  • Cossar, J. D., Rowell, P., and Stewart, W. D. P. (1984). Thioredoxin as a modulator of glucose-6-phosphate dehydrogenase in a N2-fixing cyanobacterium. J. Gen. Microbiol., 130, 991–998.

    CAS  Google Scholar 

  • Cumino, A., Curatti, L., Giarrocco, L., and Salerno, G. L. (2002). Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution. FEBS Lett., 517, 19–23.

    Article  CAS  PubMed  Google Scholar 

  • Cumino, A., Ekeroth, C., and Salerno, G. L. (2001). Sucrose-phosphate phosphatase from Anabaena sp. strain PCC 7120: isolation of the protein and gene revealed significant structural differences from the higher-plant enzyme. Planta (Berlin), 214, 250–256.

    CAS  Google Scholar 

  • Curatti, L., Flores, E., and Salerno, G. (2002). Sucrose is involved in the diazotrophic metabolism of the heterocysts-forming cyanobacterium Anabaena sp. FEBS Lett., 513, 175–178.

    Article  CAS  PubMed  Google Scholar 

  • Curatti, L., Folco, E., Desplats, P., Abratti, G., Limones, V., Herrera-Estrella, L., et al. (1998). Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli. J. Bacteriol., 180, 6776–6779.

    CAS  PubMed  Google Scholar 

  • Curatti, L., Porchia, A. C., Herrera-Estrella, L., and Salerno, G. L. (2000). A prokaryotic sucrose synthase gene (susA) isolated from a filamentous nitrogen-fixing cyanobacterium encodes a protein similar to those of plants. Planta (Berlin), 211, 729–735.

    CAS  Google Scholar 

  • Dean, D. R., and Jacobson, M. R. (1992). Biochemical genetics of nitrogenase. In G. Stacey, R. H. Burris and H. J. Evans (Eds.), Biological nitrogen fixation (pp. 763–834). New York: Chapman and Hall.

    Google Scholar 

  • Deom, C. M., Schubert, K. R., Wolf, S., Holt, C. A., Lucas, W. J., and Beachy, R. N. (1990). Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Proc. Natl. Acad. Sci. U.S.A., 87, 3284–3288.

    CAS  PubMed  Google Scholar 

  • Dong, Y., Huang, X., Wu, X.-Y., and Zhao, J. (2000). Identification of the active site of HetR protease and its requirement for heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol., 182, 1575–1579.

    Article  CAS  PubMed  Google Scholar 

  • Drews, G., and Weckesser, J. (1982). Function, structure and composition of cell walls and external layers. In N. G. Carr and B. A. Whitton (Eds.), The biology of cyanobacteria (pp. 333–357). Blackwell Scientific Publications, Ltd., Oxford, United Kingdom.

    Google Scholar 

  • Elhai, J., and Wolk, C. P. (1990). Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabaena. EMBO J., 9, 3379–3388.

    CAS  PubMed  Google Scholar 

  • Ernst, A., Black, T. A., Cai, Y. P., Panoff, J. M., Tiwari, D. N., and Wolk, C. P. (1992). Synthesis of nitrogenase in mutants of the cyanobacterium Anabaena sp. strain PCC 7120 affected in heterocyst development or metabolism. J. Bacteriol., 174, 6025–6032.

    CAS  PubMed  Google Scholar 

  • Fadi Aldehni, M., Sauer, J., Spielhaupter, C., Schmid, R., and Forchhammer, K. (2003). Signal transduction protein P(II) is required for NtcA-regulated gene expression during nitrogen deprivation in the cyanobacterium Synechococcus elongatus strain PCC 7942. J. Bacteriol., 185, 2582–2591.

    Article  CAS  PubMed  Google Scholar 

  • Fay, P. (1992). Oxygen relations of nitrogen fixation in cyanobacteria. Microbiol. Rev., 56, 340–373.

    CAS  PubMed  Google Scholar 

  • Fay, P., and Walsby, A. E. (1966). Metabolic activities of isolated heterocysts of the blue-green alga Anabaena cylindrica. Nature, 209, 94–95.

    CAS  PubMed  Google Scholar 

  • Fernández-Piňas, F., Leganés, F., and Wolk, C. P. (1994). A third genetic locus required for the formation of heterocysts in Anabaena sp. strain PCC 7120. J. Bacteriol., 176, 5277–5283.

    PubMed  Google Scholar 

  • Fiedler, G., Arnold, M., Hannus, S., and Maldener, I. (1998a). The DevBCA exporter is essential for envelope formation in heterocysts of the cyanobacterium Anabaena sp. strain PCC 7120. Mol. Microbiol., 27, 1193–1202.

    Article  CAS  PubMed  Google Scholar 

  • Fiedler, G., Arnold, M., and Maldener, I. (1998b). Sequence and mutational analysis of the devBCA gene cluster encoding a putative ABC transporter in the cyanobacterium Anabaena variabilis ATCC 29413. Biochim. Biophys. Acta, 1375, 140–143.

    CAS  PubMed  Google Scholar 

  • Fiedler, G., Muro-Pastor, A. M., Flores, E., and Maldener, I. (2001). NtcA-dependent expression of the devBCA operon, encoding a heterocyst-specific ATP-binding cassette transporter in Anabaena spp. J. Bacteriol., 183, 3795–3799.

    Article  CAS  PubMed  Google Scholar 

  • Flores, E., and Herrero, A. (1994). Assimilatory nitrogen metabolism and its regulation. In D. Bryant (Ed.), The molecular biology of cyanobacteria (pp. 487–517). Dordrecht, Netherlands: Kluwer Academic Publishers.

    Google Scholar 

  • Forchhammer, K., and Hedler, A. (1997). Phosphoprotein PII from cyanobacteria: analysis of functional conservation with the PII signal-transduction protein from Escherichia coli. Eur. J. Biochem., 244, 869–875.

    Article  CAS  PubMed  Google Scholar 

  • Forchhammer, K., and Tandeau de Marsac, N. (1995a). Functional analysis of the phosphoprotein PII (glnB gene product) in the cyanobacterium Synechococcus sp. strain PCC 7942. J. Bacteriol., 177, 2033–2040.

    CAS  PubMed  Google Scholar 

  • Forchhammer, K., and Tandeau de Marsac, N. (1995b). Phosphorylation of the PII protein (glnB gene product) in the cyanobacterium Synechococcus sp. strain PCC 7942: analysis of in vitro kinase activity. J. Bacteriol., 177, 5812–5817.

    CAS  PubMed  Google Scholar 

  • Foulds, I. J., and Carr, N. G. (1977). A proteolytic enzyme degrading phycocyanin in the cyanobacterium Anabaena cylindrica. FEMS Microbiol. Lett., 2, 117–119.

    Article  CAS  Google Scholar 

  • Frýas, J. E., Flores, E., and Herrero, A. (1994). Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. PCC 7120. Mol. Microbiol., 14, 823–832.

    Google Scholar 

  • Frýas, J. E., Flores, E., and Herrero, A. (1997). Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol., 179, 477–486.

    Google Scholar 

  • Frýas, J. E., Flores, E., and Herrero, A. (2000). Activation of the Anabaena nir operon promoter requires both NtcA (CAP family) and NtcB (LysR family) transcription factors. Mol. Microbiol., 38, 613–625.

    Google Scholar 

  • Frýas, J. E., Merida, A., Herrero, A., Martin-Nieto, J., and Flores, E. (1993). General distribution of the nitrogen control gene ntcA in cyanobacteria. J. Bacteriol., 175, 5710–5713.

    Google Scholar 

  • Gambacorta, A., Soriente, A., Trincone, A., and Sodano, G. (1995). Biosynthesis of the heterocyst glycolipids in the cyanobacterium Anabaena cylindrica. Phytochem., 39, 771–774.

    Article  CAS  Google Scholar 

  • Giddings, T. H., Jr., and Staehelin, L. A. (1978). Plasma membrane architecture of Anabaena cylindrica: occurrence of microplasmodesmata and changes associated with heterocyst development and the cell cycle. Cytobiol., 16, 235–249.

    Google Scholar 

  • Golden, J. W. (2002). PatS peptide inhibits heterocyst development and influences pattern formation in Anabaena. Biol. Environ.-Proc. Royal Irish Acad., 102B, 57–59.

    Google Scholar 

  • Golden, J. W., Carrasco, C. D., Mulligan, M. E., Schneider, G. J., and Haselkorn, R. (1988). Deletion of a 55-kilobase-pair DNA element from the chromosome during heterocyst differentiation of Anabaena sp. strain PCC 7120. J. Bacteriol., 170, 5034–5041.

    CAS  PubMed  Google Scholar 

  • Golden, J. W., Robinson, S. J., and Haselkorn, R. (1985). Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium Anabaena. Nature, 314, 419–423.

    Article  CAS  PubMed  Google Scholar 

  • Golden, J. W., and Wiest, D. R. (1988). Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene. Science, 242, 1421–1423.

    CAS  PubMed  Google Scholar 

  • Golden, J. W., and Yoon, H.-S. (1998). Heterocyst formation in Anabaena. Curr. Opin. Microbiol., 1, 623–629.

    CAS  PubMed  Google Scholar 

  • Hagen, K. D., and Meeks, J. C. (1999). Biochemical and genetic evidence for participation of DevR in a phosphorelay signal transduction pathway essential for heterocyst maturation in Nostoc punctiforme ATCC 29133. J. Bacteriol., 181, 4430–4434.

    CAS  PubMed  Google Scholar 

  • Hanson, T. E., Forchhammer, K., Tandeau de Marsac, N., and Meeks, J. C. (1998). Characterization of the glnB gene product of Nostoc punctiforme strain ATCC 29133: glnB or the PII protein may be essential. Microbiol., 144, 1537–1547.

    CAS  Google Scholar 

  • Happe, T., Schütz, K., and Böhme, H. (2000). Transcriptional and mutational analysis of the uptake hydrogenase of the filamentous cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol., 182, 1624–1631.

    Article  CAS  PubMed  Google Scholar 

  • Haselkorn, R. (1998). How cyanobacteria count to 10. Science, 283, 891–892.

    Google Scholar 

  • Hebbar, P. B., and Curtis, S. E. (2000). Characterization of devH, a gene encoding a putative DNA binding protein required for heterocyst function in Anabaena sp. strain PCC 7120. J. Bacteriol., 182, 3572–3581.

    Article  CAS  PubMed  Google Scholar 

  • Heinlein, M., Wood, M. R., Thiel, T., and Beachy, R. N. (1998). Targeting and modification of prokaryotic cell-cell junctions by tobacco mosaic virus cell-to-cell movement protein. Plant J., 14, 345–351.

    Article  CAS  PubMed  Google Scholar 

  • Herrero, A., Muro-Pastor, A. M., and Flores, E. (2001). Nitrogen control in cyanobacteria. J. Bacteriol., 183, 411–425.

    Article  CAS  PubMed  Google Scholar 

  • Hill, D. R., Belbin, T. J., Thorsteinsson, M. V., Bassam, D., Brass, S., Ernst, A., et al. (1996). GlbN (cyanoglobin) is a peripheral membrane protein that is restricted to certain Nostoc spp. J. Bacteriol., 178, 6587–6598.

    CAS  PubMed  Google Scholar 

  • Hisbergues, M., Jeanjean, R., Joset, F., Tandeau de Marsac, N., and Bedu, S. (1999). Protein PII regulates both inorganic carbon and nitrate uptake and is modified by a redox signal in Synechocystis PCC 6803. FEBS Lett., 463, 216–220.

    Article  CAS  PubMed  Google Scholar 

  • Holland, D., and Wolk, C. P. (1990). Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocysts. J. Bacteriol., 172, 3131–3137.

    CAS  PubMed  Google Scholar 

  • Huang, T.-C., Lin, R.-F., Chu, M.-K., and Chen, H.-M. (1999). Organization and expression of nitrogen-fixation genes in the aerobic nitrogen-fixing unicellular cyanobacterium Synechococcus sp. strain RF-1. Microbiol., 145, 743–753.

    CAS  Google Scholar 

  • Irmler, A., and Forchhammer, K. (2001). A PP2C-type phosphatase dephosphorylates the PII signaling protein in the cyanobacterium Synechocystis PCC 6803. Proc. Natl. Acad. Sci. U.S.A., 98, 12978–12983.

    Article  CAS  PubMed  Google Scholar 

  • Irmler, A., Sanner, S., Dierks, H., and Forchhammer, K. (1997). Dephosphorylation of the phospho-protein PII in Synechococcus PCC 7942: identification of an ATP and 2-oxoglutarate-regulated phosphatase activity. Mol. Microbiol., 26, 81–90.

    Article  CAS  PubMed  Google Scholar 

  • Jiang, F., Mannervik, B., and Bergman, B. (1997). Evidence for redox regulation of the transcription factor NtcA, acting both as an activator and a repressor, in the cyanobacterium Anabaena PCC 7120. Biochem. J., 327, 513–517.

    CAS  PubMed  Google Scholar 

  • Jiang, F., Wisen, S., Widersten, M., Bergman, B., and Mannervik, B. (2000). Examination of the transcription factor NtcA-binding motif by in vitro selection of DNA sequences from a random library. J. Mol. Biol., 301, 783–793.

    Article  CAS  PubMed  Google Scholar 

  • Johnson, T. R., Haynes, J. I., Wealand, J. L., Yarbrough, L. R., and Hirschberg, R. (1988). Structure and regulation of genes encoding phycocyanin and allophycocyanin from Anabaena variabilis ATCC 29413. J. Bacteriol., 170, 1858–1865.

    CAS  PubMed  Google Scholar 

  • Jones, K. M., and Haselkorn, R. (2002). Newly identified cytochrome c oxidase operon in the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 specifically induced in heterocysts. J. Bacteriol., 184, 2491–2499.

    CAS  PubMed  Google Scholar 

  • Kaneko, T., Nakamura, Y., Wolk, C. P., Kuritz, T., Sasamoto, S., Watanabe, A., et al. (2001). Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res., 8, 205–213; 227–253.

    CAS  PubMed  Google Scholar 

  • Khudyakov, I. Y., and Golden, J. W. (2001). Identification and inactivation of three group 2 sigma factor genes in Anabaena sp. strain PCC 7120. J. Bacteriol., 183, 6667–6675.

    Article  CAS  PubMed  Google Scholar 

  • Khudyakov, I. Y., and Wolk, C. P. (1996). Evidence that the hanA gene coding for HU protein is essential for heterocyst differentiation in, and cyanophage A-4(L) sensitivity of, Anabaena sp. strain PCC 7120. J. Bacteriol., 178, 3572–3577.

    CAS  PubMed  Google Scholar 

  • Khudyakov, I. Y., and Wolk, C. P. (1997). hetC, a gene coding for a protein similar to bacterial ABC protein exporters, is involved in early regulation of heterocyst differentiation in Anabaena sp. strain PCC 7120. J. Bacteriol., 179, 6971–6978.

    CAS  PubMed  Google Scholar 

  • Kikuchi, H., Aichi, M., Suzuki, I., and Omata, T. (1996). Positive regulation by nitrite of the nitrate assimilation operon in the cyanobacteria Synechococcus sp. strain PCC 7942 and Plectonema boryanum. J. Bacteriol., 178, 5822–5825.

    CAS  PubMed  Google Scholar 

  • Koksharova, O. A., and Wolk, C. P. (2002). Novel DNA-binding proteins in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol., 184, 3931–3940.

    CAS  PubMed  Google Scholar 

  • Lambein, F., and Wolk, C. P. (1973). Structural studies on the glycolipids from the envelope of the heterocyst of Anabaena cylindrica. Biochemistry, 12, 791–798.

    Article  CAS  PubMed  Google Scholar 

  • Lázaro, S., Fernández-Piáas, F., Fernández-Valiente, E., Blanco-Rivero, A., and Leganés, F. (2001). pbpB, a gene coding for a putative penicillin-binding protein, is required for aerobic nitrogen fixation in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol., 183, 628–636.

    PubMed  Google Scholar 

  • Lee, H. M., Flores, E., Forchhammer, K., Herrero, A., and Tandeau de Marsac, N. (2000). Phosphorylation of the signal transducer PII protein and an additional effector are required for the PII-mediated regulation of nitrate and nitrite uptake in the cyanobacterium Synechococcus sp. PCC 7942. Eur. J. Biochem., 267, 591–600.

    CAS  PubMed  Google Scholar 

  • Lee, H. M., Flores, E., Herrero, A., Houmard, J., and Tandeau de Marsac, N. (1998). A role for the signal transduction protein PII in the control of nitrate/nitrite uptake in a cyanobacterium. FEBS Lett., 427, 291–295.

    Article  CAS  PubMed  Google Scholar 

  • Lee, H. M., Vázquez-Bermúdez, M. F., and Tandeau de Marsac, N. (1999). The global nitrogen regulator NtcA regulates transcription of the signal transducer PII (GlnB) and influences its phosphorylation level in response to nitrogen and carbon supplies in the cyanobacterium Synechococcus sp. strain PCC 7942. J. Bacteriol. 181, 2697–2702.

    CAS  PubMed  Google Scholar 

  • Leganés, F. (1994). Genetic evidence that hepA gene is involved in the normal deposition of the envelope of both heterocysts and akinetes in Anabaena variabilis ATCC 29413. FEMS Microbiol. Lett., 123, 63–67.

    PubMed  Google Scholar 

  • Li, B., Huang, X., and Zhao, J. (2002). Expression of hetN during heterocyst differentiation and its inhibition of hetR up-regulation in the cyanobacterium Anabaena sp. PCC 7120. FEBS Lett., 517, 87–91.

    Article  CAS  PubMed  Google Scholar 

  • Liang, J. H., Scappino, L., and Haselkorn, R. (1992). The patA gene product, which contains a region similar to cheY of Escherichia coli, controls heterocyst pattern formation in the cyanobacterium Anabaena 7120. Proc. Natl. Acad. Sci. U.S.A., 89, 5655–5659.

    CAS  PubMed  Google Scholar 

  • Liu, D., and Golden, J. W. (2002). hetL overexpression stimulates heterocyst formation in Anabaena sp. strain PCC 7120. J. Bacteriol., 184, 6873–6881.

    CAS  PubMed  Google Scholar 

  • Lockau, W., Massalsky, B., and Dirmeier, A. (1988). Purification and partial characterization of a calcium-stimulated protease from the cyanobacterium, Anabaena variabilis. Eur J. Biochem., 172, 433–438.

    Article  CAS  PubMed  Google Scholar 

  • Lyons, E. M. and Thiel, T. (1995). Characterization of nifB, nifS, and nifU genes in the cyanobacterium Anabaena variabilis: NifB is required for the vanadium-dependent nitrogenase. J. Bacteriol., 177, 1570–1575.

    CAS  PubMed  Google Scholar 

  • Maldener, I., Fiedler, G., Ernst, A., Fernandez-Pinas, F., and Wolk, C. P. (1994). Characterization of devA, a gene required for the maturation of proheterocysts in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol., 176, 7543–7549.

    CAS  PubMed  Google Scholar 

  • Maldener, I., Lockau, W., Cai, Y. P., and Wolk, C. P. (1991). Calcium-dependent protease of the cyanobacterium Anabaena: molecular cloning and expression of the gene in Escherichia coli, sequencing and site-directed mutagenesis. Mol. Gen. Genet., 225, 113–120.

    Article  CAS  PubMed  Google Scholar 

  • Masepohl, B., Schölisch, K., Görlitz, K., Kutzki, C., and Böhme, H. (1997). The heterocyst-specific fdxH gene product of the cyanobacterium Anabaena sp. PCC 7120 is important but not essential for nitrogen fixation. Mol. Gen. Genet., 253, 770–776.

    CAS  PubMed  Google Scholar 

  • Masukawa, H., Mochimaru, M., and Sakurai, H. (2002). Disruption of the uptake hydrogenase gene, but not of the bidirectional hydrogenase gene, leads to enhanced photobiological hydrogen production by the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120. Appl. Microbiol. Biotech., 58, 618–624.

    CAS  Google Scholar 

  • Meeks, J. C, and Elhai, J. (2002). Regulation of cellular differentiation in filamentous cyanobacteria in free-living and plant-associated symbiotic growth states. Microbiol. Mol. Biol. Rev., 66, 94–121.

    CAS  PubMed  Google Scholar 

  • Meeks, J. C., Elhai, J., Thiel, T., Potts, M., Larimer, F., Lamerdin, J., et al. (2001). An overview of the genome of Nostoc punctiforme, a multicellular, symbiotic cyanobacterium. Photosyn. Res., 70, 85–106.

    CAS  PubMed  Google Scholar 

  • Meeks, J. C., Joseph, C. M., and Haselkorn, R. (1988). Organization of the nif genes in cyanobacteria in symbiotic association with Azolla and Anthoceros. Arch. Microbiol., 150, 61–71.

    Article  CAS  PubMed  Google Scholar 

  • Meeks, J. C., Wolk, C. P., Lockau, W., Schilling, N., Shaffer, P. W., and Chien, W.-S. (1978). Pathways of assimilation of 13NH4+ by cyanobacteria with and without heterocysts. J. Bacteriol., 134, 125–130.

    CAS  PubMed  Google Scholar 

  • Meeks, J. C., Wolk, C. P., Thomas, J., Lockau, W., Shaffer, P. W., Austin, S. M., et al. (1977). The pathways of assimilation of 13NH4+ by the cyanobacterium, Anabaena cylindrica. J. Biol. Chem., 252, 7894–7900.

    CAS  PubMed  Google Scholar 

  • Meeks, J. C., Wycoff, K. L., Chapman, J. S., and Enderlin, C. S. (1983). Regulation of expression of nitrate and dinitrogen assimilation by Anabaena species. Appl. Environ. Microbiol., 45, 1351–1359.

    CAS  PubMed  Google Scholar 

  • Montesinos, M. L., Muro-Pastor, A. M., Herrero, A. and Flores, E. (1998). Ammonium/methyl-ammonium permeases of a cyanobacterium. Identification and analysis of three nitrogen-regulated amt genes in Synechocystis sp. PCC 6803. J. Biol. Chem., 273, 31463–31470.

    Article  CAS  PubMed  Google Scholar 

  • Muro-Pastor, A. M., and Florencio, F. J. (1994). NADP+-isocitrate dehydrogenase from the cyanobacterium Anabaena sp. strain PCC 7120: purification and characterization of the enzyme and cloning, sequencing, and disruption of the icd gene. J. Bacteriol., 176, 2718–2726.

    CAS  PubMed  Google Scholar 

  • Muro-Pastor, A. M., Reyes, J. C., and Florencio, F. J. (1996). The NADP+-isocitrate dehydrogenase gene (icd) is nitrogen regulated in cyanobacteria. J. Bacteriol., 178, 4070–4076.

    CAS  PubMed  Google Scholar 

  • Muro-Pastor, A. M., Reyes, J. C., and Florencio, F. J. (2001). Cyanobacteria perceive nitrogen status by sensing intracellular 2-oxoglutarate levels. J. Biol. Chem., 276, 38320–38328.

    CAS  PubMed  Google Scholar 

  • Muro-Pastor, A. M., Valladares, A., Flores, E., and Herrero, A. (1999). The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development. J. Bacteriol., 181, 6664–6669.

    CAS  PubMed  Google Scholar 

  • Muro-Pastor, A. M., Valladares, A., Flores, E., and Herrero, A. (2002). Mutual dependence of the expression of the cell differentiation regulatory protein HetR and the global nitrogen regulator NtcA during heterocyst development. Mol. Microbiol., 44, 1377–1385.

    Article  CAS  PubMed  Google Scholar 

  • Murry, M. A., Olafsen, A. G., and Benemann, J. R. (1981). Oxidation of diaminobenzidine in the heterocysts of Anabaena cylindrica. Curr Microbiol., 6, 201–206.

    Article  CAS  Google Scholar 

  • Murry, M. A., and Wolk, C. P. (1989). Evidence that the barrier to the penetration of oxygen into heterocysts depends upon two layers of the cell envelope. Arch. Microbiol., 151, 469–474.

    Article  CAS  Google Scholar 

  • Oxelfelt, F., Tamagnini, P., and Lindblad, P. (1998). Hydrogen uptake in Nostoc sp. strain PCC 73102. Cloning and characterization of hupSL homologue. Arch. Microbiol., 169, 267–274.

    Article  CAS  PubMed  Google Scholar 

  • Potts, M., Angeloni, S. V., Ebel, R. E., and Bassam, D. (1992). Myoglobin in a cyanobacterium. Science, 256, 1690–1692.

    CAS  PubMed  Google Scholar 

  • Ramasubramanian, T. S., Wei, T.-F., and Golden, J. W. (1994). Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell-and heterocyst-specific genes. J. Bacteriol., 176, 1214–1223.

    CAS  PubMed  Google Scholar 

  • Ramasubramanian, T. S., Wei, T.-F., Oldham, A. K., and Golden, J. W. (1996). Transcription of the Anabaena sp. strain PCC 7120 ntcA gene: multiple transcripts and NtcA binding. J. Bacteriol., 178, 922–926.

    CAS  PubMed  Google Scholar 

  • Ramaswamy, K. S., Carrasco, C. D., Fatma, T., and Golden, J. W. (1997). Cell-type specificity of the Anabaena fdxN-element rearrangement requires xisH and xisI. Mol. Microbiol., 23, 1241–9.

    Article  CAS  PubMed  Google Scholar 

  • Reyes, J. C., and Florencio, F. J. (1994). A new type of glutamine synthetase in cyanobacteria — the protein encoded by the glnN gene supports nitrogen assimilation in Synechocystis sp. strain PCC 6803. J. Bacteriol., 176, 1260–1267.

    CAS  PubMed  Google Scholar 

  • Richaud, C., Zabulon, G., Joder, A., and Thomas, J.-C. (2001). Nitrogen or sulfur starvation differentially affects phycobilisome degradation and expression of the nblA gene in Synechocystis strain PCC 6803. J. Bacteriol., 183, 2989–2994.

    Article  CAS  PubMed  Google Scholar 

  • Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M., and Stanier, R. Y. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol., 111, 1–61.

    Google Scholar 

  • Rippka, R., and Stanier, R. Y. (1978). The effects of anaerobiosis on nitrogenase synthesis and heterocyst development by Nostocacean cyanobacteria. J. Gen. Microbiol., 105, 83–94.

    CAS  Google Scholar 

  • Ruppert, U., Irmler, A., Kloft, N., and Forchhammer, K. (2002). The novel protein phosphatase PphA from Synechocystis PCC 6803 controls dephosphorylation of the signalling protein PII. Mol. Microbiol., 44, 855–864.

    Article  CAS  PubMed  Google Scholar 

  • Sakamoto T., Inoue-Sakamoto, K., and Bryant D. A. (1999). A novel nitrate/nitrite permease in the marine cyanobacterium Synechococcus sp. strain PCC 7002. J. Bacteriol., 181, 7363–7372.

    CAS  PubMed  Google Scholar 

  • Sato, N. (1994). A cold-regulated cyanobacterial gene cluster encodes RNA-binding protein and ribosomal protein S21. Plant Mol. Biol., 24, 819–823.

    Article  CAS  PubMed  Google Scholar 

  • Schmetterer, G. (1994). Cyanobacterial respiration. In D. Bryant (Ed.), The molecular biology of cyanobacteria (pp. 409–435). Dordrecht, Netherlands: Kluwer Academic Publishers.

    Google Scholar 

  • Schmetterer, G., Valladares, A., Pils, D., Steinbach, S., Pacher, M., Muro-Pastor, A. M., et al. (2001). The coxBAC operon encodes a cytochrome c oxidase required for heterotrophic growth in the cyanobacterium Anabaena variabilis strain ATCC 29413. J. Bacteriol., 183, 6429–6434.

    Article  CAS  PubMed  Google Scholar 

  • Schmitz, O., Boison, G., Salzmann, H., Bothe, H., Schuetz, K., Wang, S.-H., et al. (2002). HoxE: a subunit specific for the pentameric bidirectional hydrogenase complex (HoxEFUYH) of cyanobacteria. Biochim. Biophys. Acta, 1554, 66–74.

    CAS  PubMed  Google Scholar 

  • Schmitz, O., and Bothe, H. (1996). The diaphorase subunit HoxU of the bidirectional hydrogenase as electron transferring protein in cyanobacterial respiration? Naturwiss., 83, 525–527.

    CAS  PubMed  Google Scholar 

  • Sherman, D. M., Tucker, D., and Sherman, L. A. (2000). Heterocyst development and localization of cyanophycin in N2-fixing cultures of Anabaena sp. PCC 7120 (cyanobacteria). J. Phycol., 36, 932–941.

    Article  CAS  Google Scholar 

  • Simon, R. (1971). Cyanophycin granules from the blue-green alga Anabaena cylindrica: a reserve material consisting of copolymers of aspartic acid and arginine. Proc. Natl. Acad. Sci. U.S.A., 68, 265–267.

    CAS  Google Scholar 

  • Soriente, A., Bisogno, T., Gambacorta, A., Romano, I., Sili, C., Trincone, A., et al. (1995). Reinvestigation of heterocyst glycolipids from the cyanobacterium, Anabaena cylindrica. Phytochem., 38, 641–645.

    Article  CAS  Google Scholar 

  • Soriente, A., Gambacorta, A., Trincone, A., Sili, C., Vincenzini, M., and Sodano, G. (1993). Heterocyst glycolipids of the cyanobacterium Cyanospira rippkae. Phytochem., 393–396.

    Google Scholar 

  • Spiller, H., Latorre, C., Hassan, M. E., and Shanmugam, K. T. (1986). Isolation and characterization of nitrogenase-derepressed mutant strains of cyanobacterium Anabaena variabilis. J. Bacteriol., 165, 412–419.

    CAS  PubMed  Google Scholar 

  • Stricker, O, Masepohl, B., Klipp, W., and Böhme, H. (1997). Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol., 179, 2930–2937.

    CAS  PubMed  Google Scholar 

  • Summers, M. L., Wallis, J. G., Campbell, E. L., and Meeks, J. C. (1995). Genetic evidence of a major role for glucose-6-phosphate dehydrogenase in nitrogen fixation and dark growth of the cyanobacterium Nostoc sp. strain ATCC 29133. J. Bacteriol., 177, 6184–6194.

    CAS  PubMed  Google Scholar 

  • Tamagnini, P., Costa, J.-L., Almeida, L., Oliveira, M.-J., Salema, R., and Lindblad, P. (2000). Diversity of cyanobacterial hydrogenases, a molecular approach. Curr Microbiol., 40, 356–361.

    Article  CAS  PubMed  Google Scholar 

  • Tandeau de Marsac, N., and Houmard, J. (1993). Adaptation of cyanobacteria to environmental stimuli-new steps towards molecular mechanisms. FEMS Microbiol. Rev., 104, 119–189.

    CAS  Google Scholar 

  • Tandeau de Marsac, N., Lee, H. M., Hisbergues, M., Castets, A. M., and Bedu, S. (2001). Control of nitrogen and carbon metabolism in cyanobacteria. J. Appl. Phycol., 13, 287–292.

    Article  CAS  Google Scholar 

  • Tanigawa, R., Shirokane, M., Maeda, S.-I., Omata, T., Tanaka, K., and Takahashi, H. (2002). Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro. Proc. Natl. Acad. Sci. U.S.A., 99, 4251–4255.

    Article  CAS  PubMed  Google Scholar 

  • Thiel, T. (1990). Protein turnover and heterocyst differentiation in the cyanobacterium Anabaena variabilis. J. Phycol., 26, 50–54.

    Article  CAS  Google Scholar 

  • Thiel, T. (1993). Characterization of genes for an alternative nitrogenase in the cyanobacterium Anabaena variabilis. J. Bacteriol., 175, 6276–6286.

    CAS  PubMed  Google Scholar 

  • Thiel, T. (1996). Isolation and characterization of the vnfEN genes of the cyanobacterium Anabaena variabilis. J. Bacteriol., 178, 4493–4499.

    CAS  PubMed  Google Scholar 

  • Thiel, T., and Leone, M. (1986) Effect of glutamine on growth and heterocyst differentiation in the cyanobacterium Anabaena variabilis. J. Bacteriol., 168, 769–774.

    CAS  PubMed  Google Scholar 

  • Thiel, T., Lyons, E. M., and Erker, J. E. (1997). Characterization of genes for a second Mo-dependent nitrogenase in the cyanobacterium Anabaena variabilis. J. Bacteriol., 179, 5222–5225.

    CAS  PubMed  Google Scholar 

  • Thiel, T., Lyons, E. M., Erker, J. E., and Ernst, A. (1995). A second nitrogenase in vegetative cells of a heterocyst-forming cyanobacterium. Proc. Natl. Acad. Sci. U.S.A., 92, 9358–9362.

    CAS  PubMed  Google Scholar 

  • Thiel, T., Lyons, E. M., and Thielemier, J. (1998). Organization and regulation of two clusters of nif genes in the cyanobacterium Anabaena variabilis. In L. W. Peschek, G. Schmetterer (Ed.), Phototrophic prokaryotes (pp. 517–521). New York: Plenum Press.

    Google Scholar 

  • Thiel, T., and Pratte, B. (2001). Effect on heterocyst differentiation of nitrogen fixation in vegetative cells of the cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol., 183, 280–286.

    Article  CAS  PubMed  Google Scholar 

  • Thiel, T., Pratte, B., and Zahalak, M. (2002). Transport of molybdate in the cyanobacterium Anabaena variabilis ATCC 29413. Arch. Microbiol., 179, 50–56.

    Article  CAS  PubMed  Google Scholar 

  • Thomas, J., Meeks, J. C., Wolk, C. P., Shaffer, P. W., and Austin, S. M. (1977). Formation of glutamine from [13N]ammonia, [13N]dinitrogen, and [14C]glutamate by heterocysts isolated from Anabaena cylindrica. J. Bacteriol., 129, 1545–1555.

    CAS  PubMed  Google Scholar 

  • Tumer, N. E., Robinson, S. J., and Haselkorn, R. (1983). Different promoters for the Anabaena glutamine synthetase gene during growth using molecular or fixed nitrogen. Nature, 306, 337–342.

    Article  CAS  Google Scholar 

  • Udvardy, J., Borbëly, G., Juhäsz, A., and Farkas, G. L. (1984). Thioredoxins and the redox modulation of glucose-6-phosphate dehydrogenase in Anabaena sp. strain PCC 7120 vegetative cells and heterocysts. J. Bacteriol., 157, 681–683.

    CAS  PubMed  Google Scholar 

  • Valladares, A., Montesinos, M. L., Herrero, A., and Flores, E. (2002). An ABC-type, high-affinity urea permease identified in cyanobacteria. Mol. Microbiol., 43, 703–715.

    Article  CAS  PubMed  Google Scholar 

  • Valladares, A., Muro-Pastor, A., Fillat, M., Herrero, A., and Flores, E. (1999). Constitutive and nitrogen-regulated promoters of the petH gene encoding ferredoxin:NADP+ reductase in the heterocyst-forming cyanobacterium Anabaena sp. FEBS Lett., 449, 159–164.

    Article  CAS  PubMed  Google Scholar 

  • Vazquez-Bermudez, M. F., Herrero, A., and Flores, E. (2002a). 2-Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter. FEBS Lett., 512, 71–74.

    CAS  PubMed  Google Scholar 

  • Vazquez-Bermudez, M. F., Paz-Yepes, J., Herrero, A., and Flores, E. (2002b). The NtcA-activated amt1 gene encodes a permease required for uptake of low concentrations of ammonium in the cyanobacterium Synechococcus sp. PCC 7942. Microbiol., 148, 861–869.

    CAS  Google Scholar 

  • Vega-Palas, M. A., Flores, E., and Herrero, A. (1992). NtcA, a global nitrogen regulator from the cyanobacterium Synechococcus that belongs to the Crp family of bacterial regulators. Mol. Microbiol., 6, 1853–1859.

    CAS  PubMed  Google Scholar 

  • Walsby, A. (1985). The permeability of heterocysts to the gases nitrogen and oxygen. Proc. Royal Soc. (London) B, 226, 345–366.

    CAS  Google Scholar 

  • Wang, Q., Li, H., and Post, A. F. (2000). Nitrate assimilation genes of the marine diazotrophic, filamentous cyanobacterium Trichodesmium sp. strain WH9601. J. Bacteriol., 182, 1764–1767.

    CAS  PubMed  Google Scholar 

  • Wealand, J. L., Myers, J. A., and Hirschberg, R. (1989). Changes in gene expression during nitrogen starvation in Anabaena variabilis ATCC 29413. J. Bacteriol., 171, 1309–1313.

    CAS  PubMed  Google Scholar 

  • Weckesser, J., and Jürgens U. J. (1988). Cell walls and external layers. Meth. Enzymol., 167, 173–188.

    CAS  Google Scholar 

  • Wei, T.-F., Ramasubramanian, T. S., and Golden, J. W. (1994). Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development. J. Bacteriol., 176, 4473–4482.

    CAS  PubMed  Google Scholar 

  • Wei, T.-F., Ramasubramanian, T. S., Pu, F., and Golden, J. W. (1993). Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection. J. Bacteriol., 175, 4025–4035.

    CAS  PubMed  Google Scholar 

  • Wilcox, M., Mitchison, G. J., and Smith, R. J. (1973a). Pattern formation in the blue-green alga, Anabaena: I. Basic mechanism. J. Cell Sci., 12, 707–725.

    CAS  PubMed  Google Scholar 

  • Wilcox, M., Mitchison, G. J., and Smith, R. J. (1973b). Pattern formation in the blue-green alga, Anabaena: II. Controlled proheterocyst regression. J. Cell Sci., 13, 637–649.

    CAS  PubMed  Google Scholar 

  • Winkenbach, F., Wolk, C. P., and Jost, M. (1972). Lipids of membranes and of the cell envelope in heterocysts of a blue-green alga. Planta, 107, 69–80.

    Article  CAS  Google Scholar 

  • Wolfinger, E. D., and Bishop, P. E. (1991). Nucleotide sequence and mutational analysis of the vnfENX region of Azotobacter vinelandii. J. Bacteriol., 173, 7565–7572.

    CAS  PubMed  Google Scholar 

  • Wolk, C. P. (1973). Physiology and cytological chemistry blue-green algae. Bacteriol. Rev., 37, 32–101.

    CAS  PubMed  Google Scholar 

  • Wolk, C. P. (1982). Heterocysts. In N. G. Carr and B. A. Whitton (Eds.), The biology of cyanobacteria (pp. 359–386). London: Blackwell Scientific Publishers.

    Google Scholar 

  • Wolk, C. P. (2000). Heterocyst formation in Anabaena, In Y. Brun, and L. Shimkets (Eds.), Prokaryotic development (pp. 83–104). Washington, DC: American Society for Microbiology.

    Google Scholar 

  • Wolk, C. P., Ernst, A., and Elhai, J. (1994). Heterocyst metabolism and development. In D. Bryant (Ed.), The molecular biology of cyanobacteria (pp. 769–823). Dordrecht: Kluwer Academic Publishers.

    Google Scholar 

  • Wolk, C. P., and Quine, M. P. (1975). Formation of one-dimensional patterns by stochastic processes and by filamentous blue-green algae. Dev. Biol., 46, 370–382.

    Article  CAS  PubMed  Google Scholar 

  • Wolk, C. P., Thomas, J., Shaffer, P. W., Austin, S. M., and Galonsky, A. (1976). Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica. J. Biol. Chem., 251, 5027–5034.

    CAS  PubMed  Google Scholar 

  • Wolk, C. P., Zhu, J., and Kong, R. (1999). Genetic analysis of heterocyst formation. In G. A. Peschek, W. Löffelhardt, and G. Schmetterer (Eds.), The phototrophic prokaryotes. (pp. 509–515). Kluwer Academic Publishers, New York, N.Y.

    Google Scholar 

  • Wong, F. C. Y., and Meeks, J. C. (2001). The hetF gene product is essential to heterocyst differentiation and affects HetR function in the cyanobacterium Nostoc punctiforme. J. Bacteriol., 183, 2654–2661.

    CAS  PubMed  Google Scholar 

  • Wood, N. B., and Haselkorn, R. (1978). Proteinase activity during heterocyst differentiation in nitrogen-fixing cyanobacteria. In H. Holzer and G. Cohen (Eds.), Limited proteolysis in microorganisms: Biological function, use in protein structural and functional studies. (pp. 159–166). Bethesda, MD: Fogarty Intl. Center Publ.

    Google Scholar 

  • Wood, N. B., and Haselkorn, R. (1980). Control of phycobiliprotein proteolysis and heterocyst differentiation in Anabaena. J. Bacteriol., 141, 1375–1385.

    CAS  PubMed  Google Scholar 

  • Xu, X., and Wolk, C. P. (2001). Role for hetC in the transition to a nondividing state during heterocyst differentiation in Anabaena sp. J. Bacteriol., 183, 393–396.

    CAS  PubMed  Google Scholar 

  • Xu, X., Khudyakov, I., and Wolk, C. P. (1997). Lipopolysaccharide dependence of cyanophage sensitivity and aerobic nitrogen fixation in Anabaena sp. strain PCC 7120. J. Bacteriol., 179, 2884–2891.

    CAS  PubMed  Google Scholar 

  • Yoon, H.-S., and Golden, J. W. (1998). Heterocyst pattern formation controlled by a diffusible peptide. Science, 283, 935–938.

    Google Scholar 

  • Yoon, H.-S., and Golden, J. W. (2001). PatS and products of nitrogen fixation control heterocyst pattern. J. Bacteriol., 183, 2605–2613.

    Article  CAS  PubMed  Google Scholar 

  • Zahalak, M., Beachy, R. N., and Thiel, T. (1995). Expression of the movement protein of tobacco mosaic virus in the cyanobacterium Anabaena sp strain PCC 7120. Mol. Plant Microbe Interact., 8, 192–199.

    CAS  Google Scholar 

  • Zhou, R., Cao, Z., and Zhao, J. (1998a). Characterization of HetR protein turnover in Anabaena sp. PCC 7120. Arch. Microbiol., 169, 417–423.

    Article  CAS  PubMed  Google Scholar 

  • Zhou, R., Wei, X., Jiang, N., Li, H., Dong, Y., Hsi, K., et al. (1998b). Evidence that HetR protein is an unusual serine-type protease. Proc. Natl. Acad. Sci. U.S.A., 95, 4959–4963.

    CAS  PubMed  Google Scholar 

  • Zhou, R., and Wolk, C. P. (2003). A two-component system mediates developmental regulation of biosynthesis of a heterocyst polysaccharide. J. Biol. Chem., 278, 19939–19946.

    CAS  PubMed  Google Scholar 

  • Zhu, J., Jäger, K., Black, T., Zarka, K., Koksharova, O., and Wolk, C. P. (2001). HcwA, an autolysin, is required for heterocyst maturation in Anabaena sp. strain PCC 7120. J. Bacteriol., 183, 6841–6851.

    Article  CAS  PubMed  Google Scholar 

  • Zhu, J., Kong, R., and Wolk, C. P. (1998). Regulation of hepA of Anabaena sp. strain PCC 7120 by elements 5’ from the gene and by hepK. J. Bacteriol., 180, 4233–4242.

    CAS  PubMed  Google Scholar 

  • Ziegler, K., Stephan, D. P., Pistorius, E. K., Ruppel, H. G., and Lockau, W. (2001). A mutant of the cyanobacterium Anabaena variabilis ATCC 29413 lacking cyanophycin synthetase: growth properties and ultrastructural aspects. FEMS Microbiol. Lett., 196, 13–18.

    CAS  PubMed  Google Scholar 

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  1. Department of Biology, University of Missouri-St. Louis, 8801 Natural Bridge Rd., St. Louis, MO, 63121, USA

    T. Thiel

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  1. Lehrstuhl für Biologie der Mikroorganismen Fakultät für Biologie, Ruhr-Universität Bochum, Germany

    Werner Klipp  & Bernd Masepohl  & 

  2. Biochemistry Research Group, School of Biological Sciences, University of Wales, Swansea, UK

    John R. Gallon

  3. Department of Biochemistry, Virginia Polytechnic Institute & State University, Blacksburg, USA

    William E. Newton

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Thiel, T. (2004). Nitrogen Fixation in Heterocyst-Forming Cyanobacteria. In: Klipp, W., Masepohl, B., Gallon, J.R., Newton, W.E. (eds) Genetics and Regulation of Nitrogen Fixation in Free-Living Bacteria. Nitrogen Fixation: Origins, Applications, and Research Progress, vol 2. Springer, Dordrecht. https://doi.org/10.1007/1-4020-2179-8_5

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